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Two techniques were compared that uses the UV absorbance at 280nm of 0.5mol/L K_2SO_4 extracts of fumigated and unfumigated soils and fumigationwaterlogged incubation to estimate the concentrations of nitrogen in the SMB.

用氯仿熏蒸-0.5mol/L的K2SO4直接浸提,280nm紫外比色法和熏蒸-淹水培养法测定了20种有机质、全氮和速效氮差异较大的土样的土壤微生物量N。

Methods MTT was used to study the proliferation of A375 human melanoma cell. Tyrosinase activity was measured by zymologic method. The melanin was detected by its absorption at 475nm.

方法四甲基偶氮唑蓝法测定验方对细胞增殖的影响,用酶学方法测定对酪氨酸酶活性的影响,475nm比色法测定验方对黑素含量的影响。

Play recommend a Wuzhizhou, looks like a trip to 100, or 60 (tickets, not counted travel allowance), which have nothing, but there are pure white sand and blue-colored water, Yalong Bay sand and it is better than doing nothing - this is the situation a few years ago, do not know how well protected.

游玩推荐一个蜈支洲岛,貌似去一趟100还是60,里面什么都没有,但是有纯白色的沙滩和湛蓝色的海水,亚龙湾的沙子和它比什么都不是——这是几年前的状况,不知道现在保护得好不好。

Methods: After LNCaP and PC3 cell lines were affected by 10, 25, 50, 75, 100 μmol/L curcumine respectively, the cell activity was assayed with MTT method at 5, 12 and 24 hours respectively; Electronic microscopy and flow cytometry were adopted to observe the morphological changes and the cell cycle of LNCaP and PC3 cell lines at 24 hours.

分别用10、25、50、75、100μmol/L浓度的姜黄素对LNCaP及PC3前列腺癌细胞株进行体外干预,5、12、24 h后四氮甲唑蓝比色法检测细胞生长活性;24 h后透射电镜观察细胞超微结构变化,流式细胞仪测定细胞凋亡及细胞周期的变化;5 h后Western印迹法检测细胞内IκBa的表达。

Perianth lemon-colored; tube usually greenish, short, 1--2.5(--3) cm; segments spreading, 4--7.5 cm, inner ones 1.5--2.3 cm wide, wider than outer ones.

花被柠檬色;带绿色的通常筒部,短,1-2.5(-3)厘米;平展的裂片,4-7.5厘米,内部的1.5-2.3厘米宽,比外部的宽。

In order to ascertain Auto-circular fermentation technology of Mulberry vinegar and find out the vinegar 's ability to eliminate DPP H ., the liquid fermentation techniques of Mulberry vinegar, produced by using Auto-circular fermentor , have been analyzed and the micro-organism on the Mulberry fruit surface has been observed with microscope, the total flavones content as well as the free radical-DPPH eliminating ability in several kinds of vinegars have been tested.

为了确定桑椹醋自回淋发酵工艺,并了解其对 DPPH 自由基的清除能力,以秦巴山区桑椹为原料,采用液态自回流发酵法生产桑椹醋,从原料菌群、酒精发酵主产物和醋酸发酵主产物的动态变化趋势等方面对其工艺进行了分析,并采用比色法对桑椹醋总黄酮含量及其 DPPH 自由基的清除率进行了测定。

The process of the growth and morphology of the neurons were observed under phase contrast microscope in vitro.

倒置相差显微镜进行神经元形态学观察,中性红比色法和MTT法测定神经细胞的存活数量和活性,TBARS法和BCA法分别测定神经细胞中丙二醛的水平和总蛋白合成的能力。

Methods: into a series of 10 ml colorimetric tube were added different aliquots of the 1,8 -dihydroxyanthraquinone standard solution,2.0 ml of naac-hac buffer solution(ph=3.60),2.0 ml of ethylacetate.

于10.0 ml比色管中加入一定量的1,8-二羟基蒽醌标准溶液,ph值为3.60的naac-hac缓冲溶液2.0 ml,有机溶剂乙腈2.0 ml,最后定容至10.0 ml;同时做空白对照,在最大激发波长为440 nm,最大发射波长为521 nm处测定一系列溶液的荧光强度。

CoCl2 (a chemical hypoxia-mimetic agent) was used to establish the chemical hypoxia-induced PC12 cell injuries model. Sodium hydrosulfide was used as a H2S donor. The viability of PC12 cells was measured by CCK-8 assay. The percentage of apoptotic cells was assessed by propidium iodide stain flow cytometry. The morphological change of apoptotic cells was tested by using the chromatin dye Hoechst 33258. The mitochondrial membrane potential was analyzed by rhodamine 123 staining and photofluorography. The level of reactive oxygen species in PC12 cells was measured by DCFH-DA staining and photofluorography.

应用化学性缺氧模拟剂CoCl2在PC12细胞建立化学性缺氧损伤模型;以硫氢化钠作为H2S的供体;应用CCK-8比色法检测细胞存活率;碘化丙啶染色流式细胞技术检测细胞凋亡率;Hoechst33258染色检测细胞凋亡的形态学变化;罗丹明123(Rh123)染色荧光显微镜照相检测细胞线粒体膜电位;2',7'-二氯荧光黄双乙酸盐染色荧光显微镜照相检测细胞内的活性氧水平。

Redheads may actually have another trait that makes them stand out -sensitivity to pain,specialists reported .

他们在一次麻醉学专家会议上说,天然红发的人需要的麻醉剂量要比其他发色的人多20%。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口,替代木托盘,免薰蒸,符合欧盟、北美各国对出口货物包装材料的法令要求;喷涂钢托盘适用于重载上货架之用,托盘表面根据需要制作成平板状、波纹状及间隔形式,满足不同的使用要求。

A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。