比色的

Methods: The primary PAEC had been cultured and passage with porcine autoserum. PAECs were plated in 96-well microculture plates, and incubated with 5%AB normal human serum, 5% heat-inactivated FCS and 5% no heat-inactivated FCS for 6 days respectively, then the survival rates were detected by MTT chromatic test and expression of heme oxygenase 1 (HO-1) was observed by immunohistochemical method after these PAECs had been incubated with 25% NHS for 2 hours.

以猪自体血清原代培养PAEC并传代，接种于96孔板后分别以5%正常人血清、胎牛血清和补体灭活的胎牛血清诱导6d，加入25%正常人血清，以MTT比色法检测细胞的存活率，并以免疫组化的方法观察各组HO-1的表达情况。

RESULTS: Treated for a same period （3 d）, the inhibitory effect of MG132 on PC12 ceil proliferation enhanced with the increment of the concentration of MG132（0, 1, 2.5, 5, 10, 20 μmot/L）.

用不同浓度的MG132处理PC12细胞3d，通过3-（4，5-二甲基噻唑-2）-2，5-二苯基四氮唑溴盐微量比色法检测MG132对PC12细胞增殖的影响，通过姬姆萨染色法和流式细胞术检测MG132对PC12细胞凋亡的影响。

MethodsThe cells of human gastric cancer cell line SGC7901 was treated respectively with Chrysin at different concentration 10、20、40、80μM for 48h. The proliferation inhibitory rate was measured by MTT assay. Acridine orangestaining and flow cytometry were used to detect apoptosis. Western blot assay was used to detect the expression of apoptosis related genes NF拨B,Bcl2,Bax.

方法分别用10、20、40、80μM的ChR处理人胃癌细胞株SGC7901 48h，采用MTT比色法检测ChR对SGC7901细胞的增殖抑制效应；采用丫啶橙染色、流式细胞术检测ChR诱导SGC7901细胞凋亡的发生；应用Westernblot法检测凋亡相关基因NF拨B、Bcl2、Bax的蛋白表达，并用计算机图像分析软件进行半定量分析。

Control of the cell in the colorimetric device can improve the accuracy, sensitivity and the limit of detection .

在仪器比色池中采用恒温的方式不仅可以提高分析方法的灵敏度和准确度，还可以提高仪器的最低检出限。

The cardiomyocyte viability were detected by MTT assay for calculating the survival rate and the inhibitory rate of the virus. Myocardial F-actin and VEGF were analyzed by immunofluorescent staining with confocal microscopy. Mean fluorescent intensity of F-actin and VEGF were determined by flow cytometry.Results There is no toxicity on cardiomyocytes when PD is at concentration of 0.02 mmol/L and 0.2 mmol/L while there is toxicity at concentration of 2 mmol/L. CVB3 could reduce the viability of cardiomyocytes, depolymerize F-actin cytoskeleton and reorganize VEGF protein.

体外培养心肌细胞，用100 TCID50柯萨奇B3病毒（CVB3）感染心肌细胞，建立病毒性心肌炎实验模型，然后分别用0.02mmol/L、0.2mmol/L、2mmol/L三种不同浓度的PD处理感染CVB3病毒的心肌细胞，观察心肌细胞的形态和搏动，用细胞免疫荧光技术标记纤维状肌动蛋白和VEGF蛋白，四唑蓝比色法测定心肌细胞活性和病毒抑制率，流式细胞仪检测各组心肌细胞VEGF蛋白和F-actin平均荧光强度。

Objective To study the clinical curative effect of superior central incisor diastema restored with Esthet-X.Methods Restored the teeth by color compare and tooth preparation.Evaluated the degree of patients'satisfaction,teeth shapes and color generally after two years.

目的 研究Esthet-X复合树脂修复上颌中切牙牙间隙的临床效果方法通过比色、牙体预备进行牙体修复，2年后综合评价患牙充填后的形态、色泽及患者的满意度。

It presents the mechanism to Abstract total cyanide by using phosphoric acid-EDTA and the conditions to measure cyanide by using isonicotinic acid-pyrazolone colorimetry.

文章对17种含氰废水处理方法的主要原理和优缺点作了对比说明，阐述了用磷酸———EDTA提取总氰化物的机理以及用异烟酸———呲唑啉酮比色法测定氰化物的条件

Methods: The inhibitation of HL-60 cells by Rapamycin was measured by MTT assay. Morphic change of the apoptosis of HL-60 cells was observed by inverted microscope and light microscope, trapezoid straps by DNA agarose gel electrophoresis, and apoptosis rate by flow cytometry. Western-blot analysis was performed to detect the expression of Bax and Bcl-2 proteins after rapamycin treatment.

用MTT比色法测定雷帕霉素对HL-60细胞增殖的影响；用倒置显微镜和荧光显微镜观测细胞凋亡的形态学改变；DNA琼脂糖凝胶电泳观测凋亡的梯形条带；流式细胞仪检测细胞凋亡率；Western-blot检测HL-60细胞经雷帕霉素处理后，Bax、Bcl-2蛋白的表达情况。

The method uses EGCG of different pH indicator to process HT-29 cell, colorimetric of 4 Zun salt experiments (the growth of the cell after MTT) law observes processing circumstance; sheds type cell art detects cellular wither dies clean of circumstance;Western Blot detects skin grows factor accepts put oneself in another's position , cell adjusts kinase phosphation level.

方法采用不同浓度的EGCG处理HT-29细胞，四唑盐比色试验法观察处理后细胞的生长情况；流式细胞术检测细胞凋亡情况；Western Blot洁检测表皮生长因子受体、细胞外信号调节激酶磷酸化水平的表达。

Cultured for 48 hours, the floating cells were removed and the adherent cells were continued to culture with cytokines including IL-4 and GM-CSF. After 14 days culture, the induced DCs were observed by photics microscope and electron microscope, also co-cultured with native T cells derived from spleen, its stimulating function was detected by MTT assay.

经过光镜，透射电镜观察培养DCs的形态学特征；通过同种T细胞混合培养，采用MTT比色分析法测定不同浓度的DCs发同种T细胞增殖的能力。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。