比色的

The cells were shammed or irradiated with different dosages of UVB radiation and treated with the above TCM monomers. The damage of fibroblasts was observed with inverted phase contrast microscope.Cellular proliferation activity was detected by MTT method.The secretory volume of IL-6 and TNF-α was measured by ELISE. Results The damage degree of the cells irradiated was dependent with the irradiated doses and the proliferation activity reduced by 28%-44%.

采用30mJ/cm^2、60mJ/cm^2、90mJ/cm^2剂量UVB照射体外培养的原代人皮肤成纤维细胞，以中药单体EGCG和黄芩苷进行干预处理，倒置相差显微镜观察细胞受损程度，以甲唑盐比色试验检测细胞增殖活性，酶联免疫吸附试验检测上清液TNF-α、IL-6分泌量。

Methods The two different extracting methods: Ultrasonic extraction and Soxhlet's extracter were used.

分别采用超声提取法和索氏提取法，用比色法比较维药芹菜籽中总黄酮的含量。

MethodsThe alkaloid was extracted by chlorolform from Pinellia ternate and tested at 417nm with the spectroscopy by acid dye.

方法采用氯仿提取，依据酸性染料比色法的原理，在417 nm波长下测定，并作方差分析。

Extract and quantitative analysis of biological active ingredients total triterpene s in Suosuo grape.

采用正交实验设计，利用超声法从琐琐葡萄中提取生物活性成分总三萜，比色法测定总三萜的含量。

The results demonstrated that supernatant IL-6(181±22U/ml) may be found after cultivation of unstimulated HUVEC for 24 hours.

HUVEC原代培养，HUVEC释放IL-6、IL-8观察，MTT比色法作IL-6活性测定，双抗夹心ELISA法测IL-8，t检验进行统计学处理。结果未刺激的HUVEC培养24小时上清IL-6活性为 181±22U/ml。

The third passage of both HCF and HPF were measured by 3-(4,5-dimethyl-2thiazol)-2,5-diphenyl-2H-tetrazolium bromid at various days of culture.

对传第3代的两种成纤维细胞，分别于接种后2、5、8d行噻唑蓝[3-(4，5-dimethyl-2thiazol)-2，5-diphenyl-2H-tetrazolium bromid， MTT]比色试验，检测细胞增殖活力。

Methods The ARPE-19 cells were transfected with Mir-155,and then put into hypoxic chamber for 0hr、8hr、24hr、48hr,the proliferation of ARPE-19 cells was evaluated by[3-(4,5-dimethylthiazole-2yl)-2,5-diphenyl tetrazolium bromid, MTT]-test.

Mir-155转染视网膜色素上皮细胞株ARPE-19细胞后，将其置缺氧环境0hr、8hr、24hr、48hr后，MTT比色法检测ARPE-19细胞的增殖，流式细胞仪检测细胞凋亡率。

Methods With the model of aging mice induced by D-gal, the learning memory ability of mice was measured by using Y-maze, the contents of SOD and MDA in the cerebrums were determined by xanthine oxidation or TBA colorimetry ,the variations of above indices of mice after treatment with NGD were observed.

建立 D-半乳糖致小鼠衰老模型，以 Y型电迷宫检测其学习记忆能力，分别采用黄嘌呤氧化酶法、TBA比色法测定大脑 SOD活性和 MDA含量，观察小鼠服用神经生长液后上述指标的变化。

The mouse of each group were put to death by dislocating their cervical vertebras after the behavior tests.

在低温下取其脑组织，用羟胺比色法测定脑组织中Ach含量和Ach-E的活性。

The polyaluminum flocculates with different B values were prepared by addition NaOH solution to AlCl〓 solution on using different methods. The characteristics of solution chemistry and species distribution in the polyaluminum solutions were examined completely and deeply by the potentiometry,〓Al NMR, Ferron timed comlexation spectrophotometry, Na〓SO〓 precipitation and ultrafiltration method.

应用多种化学分析技术及现代分析仪器，如电位滴定、Ferron逐时络合比色、Na〓SO〓沉淀、超滤膜过滤、X-衍射、富里叶红外光谱、扫描电镜等对铝盐的水解聚合反应过程、形态分布及其结构特征进行了全面深入地分析研究。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。