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The ratio of polygonatum sibiricum Red polysaccharide extracting is the best condition in the conditions of temperature 80℃,ratio of solid-liquid 1:15,the duration of time 4h and pH 7;Having removed protein by the method of enzyme + Sevag and having decolored by adding 15% active carbon power and boiling 15min,the purity of polygonatum sibiricum red polysaccharide will be better.

在温度为80℃、固液比为1∶15、时间为4h、pH=7.0时,黄精多糖的提取率最高;通过酶+SEVAG试剂法除蛋白质,并且在粉末状活性炭用量为15%,煮沸时间为15min时脱色后可以得到较纯的黄精多糖。

The effects of the ratio of reactants, quantity of catalyst, reaction time, reaction temperature and entraining agent of water on the product yield were investigated and the reuse of catalyst was studied. The product was confirmed by GC and IR.

探讨了酸醇物质的量比、催化剂用量、反应时间、反应温度、带水剂等对反应的影响,并对催化剂进行了重复利用研究,产品经气相色谱和红外光谱分析。

By this method, the productivity of 3. 18g/l extracellular polysaccharides was obtained. The optimum extraction method was obtained by RSA. It was determined as follows: 91. 4 ℃, 2. 9h, the weight ratio between mycelia and water was 1 to 3. The productivity was 12. 38% of dry mycelia. Set the purifying methods of Grifola frondosa: Precipitated polysaccharides part by 60% ethanol→removed protein by Sevag method→removed coloring matter by H〓O〓→removed salts by dialyse→DEAE-cellulose column chromatogram→ purified polysaccharides groups.

对于胞内多糖的提取,采用湿菌体经捣碎再高压破壁的方式破碎菌体细胞,采用响应面分析法得出优化后的提取条件为:提取温度91.4℃,提取时间2.9h,料水比1:3,此提取条件下胞内粗多糖的率为干菌体重的12.38%建立了灰树花多糖分离纯化的技术路线 60%乙醇沉淀的粗多糖→Sevag法脱蛋白→H〓O〓脱色→透析脱盐→DEAE-纤维素层析分离→多糖组分。

The technical conditions for extracting oil from Xanthoceras sorbiflia Bunge seeds by microwave were studied.

以文冠果籽为原料,研究了微波辅助提取文冠果籽油的工艺条件,通过单因素试验和正交试验,探讨了溶剂种类、微波功率、提取时间、温度、料液比对文冠果籽油提取率的影响;并采用气相色谱-质谱联用方法分析了文冠果籽油的组成。

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