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比色法

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It's required for VK_1 in the present compendial monographs that the content of menadione should be strict controlled and the other impurities are controlled as related substances using colorimetry or TLC method.

目前各国药典维生素K_1的质量标准中,都是采用比色法或TLC法检查甲萘醌,而其他的杂质则作为"有关物质"同时进行检查。

Methods The cells were divided into 5 treatment groups(10,25,50,75 and 100 μmol·L-1 QUE), blank control and menstruum control group. The rat C6 cells were cultivated to 1×106·mL-1 in the RPMI 1640 medium, then added into 96 holes board with various doses of QUE by 3 holes per group,and MTT assay was used to observe the proliferation of the cells treated for 24,48 and 72 h. The change of cell cycle was also observed by flow cytometry after the cells were treated with 50 and 100 μmol·L-1 QUE for 48 h. The changes of the protein P53 and Bcl-2 of C6 cells treated with 50 μmol·L-1 QUE for 48 h were detected by immunocytochemical methods.

按QUE浓度分成10、25、50、75及100 μmol·L-15个处理组和空白对照组及溶剂对照组,大鼠脑胶质瘤C6细胞在RPMI 1640培养基中生长达1×106·mL-1后,在96孔板中分别加入上述浓度的QUE继续培养,每组设3复孔,作用24、48及72 h,采用MTT比色法检测QUE对大鼠脑胶质瘤C6细胞的增殖抑制情况,流式细胞术对50及100 μmol·L-1的QUE作用48 h的大鼠脑胶质瘤C6细胞进行周期分析,免疫组化法检测50 μmol·L-1的QUE作用48 h 的p53和bcl-2基因产物。

DFerric Perchloric acid method was used to determine the content of ester-series alkaloid, and acid dye colorimetry was used to determine the content of total alkaloid in Complex Prescription Monkshood plaster.

方法分别采用改良异羟肟酸铁-高氯酸铁法及酸性染料比色法测定制剂中的酯型生物碱及乌头类总生物碱的含量。

MethodsFerric Hydroxamic acid―Ferric Perchloric acid method was used to determine the content of ester-series alkaloid, and acid dye colorimetry was used to determine the content of total alkaloid in Complex Prescription Monkshood plaster.

方法分别采用改良异羟肟酸铁-高氯酸铁法及酸性染料比色法测定制剂中的酯型生物碱及乌头类总生物碱的含量。

Methods: The effects of Opuntia on proliferation of fibroblast were measured with MTT colorimetric assay, and PCNA productions were examined with immunocytechemistry.

以小鼠成纤维细胞株(NIH3T3)为研究对象,采用四氮唑盐比色法测定仙人掌对成纤维细胞增生的影响,采用免疫细胞化学染色法检测成纤维细胞的增殖细胞核抗原的表达。

Fig . 3 Flow chart of pentosan analysis – aniline acetate colorimetric method.

圖3聚戊醣分析法流程圖—醋酸苯胺比色法

The humanization and specificity of chimeric antibody was identified by ELISA and Western bloting, and the tumoricidal activity of the expressed chimeric antibody was detected by tetrazolium salt phenazine methosulfate assay.

采用ELISA和Western bloting法鉴定抗体的人源性和抗体-抗原结合活性,四唑盐/吩嗪硫酸甲酯比色法检测抗体的生物学活性。

Objective: To compare the reliability of phenol red colormetric, electrodes and enzymatic methods in detecting serum bicarbonate.

目的 比较酚红比色法、离子选择电极法、酶法三种全自动测定血清碳酸氢根方法的可靠性及临床应用,为临床方法的选择和实验结果的评价提供理论依据。

Results: In the comparison between phenol red colormetric method and the other methods, the corrlative coefficency was 0.9822 and 0.9813, and the bias was 1.64% and 1.59% at 15~45 mmol/L.

结果 酚红比色法与离子选择性电极法、酶法比较,相关系数分别为0.9822和0.9813,碳酸氢根测定浓度在15~45 mmol/L时的预期相对偏差均值分别为1.64%和1.59%。

The antitumor effects of sesquiterpene lactones scabertopin and isodexyelephantopin from the whole plant of Elephantopus scaber were studied in vitro on SMMC-7721,Hela and Caco-2 cancer cell lines.

采用四甲基偶氮唑盐比色法和琼脂糖凝胶电泳法等研究地胆草倍半萜内酯化合物scabertopin(1)和isodeoxyelephantopin(2)在体外对SMMC-7721、Hela和Caco-2三种肿瘤细胞增殖的影响。

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