比色法

Methods Rad ioimmunoassay and colorimeter were applied to detect the levels of serum and tum or tissue ET-1 and NO in 40 elderly cases of lung cancer and serum samples from 30 normal individuals and 20 patients with benign lung diseases were taken as c ontrols.

方法应用比色法和放免法测定 40例老年肺癌患者血清和组织中的内皮素-1和一氧化氮的含量，并设 3 0例正常人和 2 0例肺良性病变患者为对照组。

Objective: To explore the best suitable experiment condition used to compare the MTS colorimetry and the 3H-TdR incorporation.

目的：确定MTS比色法与3H-TdR掺入法在进行实验比较时的最佳实验条件。

Results Administration of L-NAME could attenuate the activity of NOS and inhibit spermatogenic cells apoptosis in rats experimental cryptorchidism, and it could significantly inhibit weight loss of the cryptorchid.

手术建立大鼠单侧隐睾模型，实验分为隐睾组、隐睾+L-NAME组［术后腹腔注射溶于生理盐水的L-NAME 50 mg/(kg\5d)］、隐睾+生理盐水组、假手术组，7 d后采用化学比色法检测睾丸组织NOS活性，流式细胞术和原位缺口末端标记法检测生精细胞凋亡，RT-PCR和免疫组织化学法检测Bax基因表达变化。

Methods Eukaryotic expression plasmid pCMVCD was constructed , and identified by re2 striction endoenzyme digestion. CD gene was transfected into NSCs from new2born Wistar rats using Lipofec2tamine2000. Positive clones (named NSCs/ CD cells) were screened by G418 presence. 52Fluorocytosine (52FC) ad2ministration of different concentrations were incubated with NSCs/ CD cells. NSCs/ CD cells viability ratios were mea2 sured by MTT assay.

通过构建真核表达质粒pCMVCD ，限制性内切酶消化鉴定后，采用Lipofectamine 2000 脂质体介导法转染新生大鼠室管膜下区神经干细胞(Neural stemcells ， NSCs)，G418 筛选阳性克隆，加入不同浓度的52氟胞嘧啶(52Flourocytosine ， 52FC)，MTT比色法测定NSCs的生存率。

After 4 passages in culture, the MSCs were implanted into 96 foveola with cell density of 0.08,0.1,0.2,0.4,0.6,0.8,1,2,4,6,8,10(x104/ml) respectively. Meanwhile, the MSCs were cultured in Dulbecco's modified Eagle's medium culture medium with different concentration of fetal bovine serum, the 3-4,5dimethylthiazol-2-yl-2,5-diphenyl tetrazelium bromide assay were used to determine the proliferation index of MSCs 72 hours later, the cell cycle were determine by cytometer also.

贴壁筛选法分离、纯化MSCs，取4代以后细胞，以不同密度接种于96孔培养板内，分别以不同浓度血清进行培养，72h后，采用四甲基偶氮唑盐比色法测定MSCs增殖能力并用流式细胞仪测定细胞周期。

Eight kinds of flowers were selected including Lily, Honeysuckle, Globe amaranth, Tribute Chrysanthemum, tea plant flower, Rose, Hibiscus sabdariffa and Saffron.

选用八种常见饮用花卉，如百合花、金银花、千日红、贡菊、茶花、玫瑰花、玫瑰茄、藏红花，分别采用电感耦合等离子体发射光谱法和三氯化铝比色法检测其微量元素和黄酮类化合物的舍量。

The effect of PP333 were explored by single factor design, colorimetry and Guaiacol process.

通过单因子实验、比色法和愈创木酚法探讨PP333对试管苗的影响。

MethodsSaccharide content was determined by anthracene ethyl ketone method;amino acid content was estimated by indene tri-ketone colorimetric method.

方法青蒿可溶性糖含量测定使用蒽酮醋酸乙酯法，青蒿游离氨基酸含量测定用茚三酮比色法。

Methods The cell growth inhibiting rate, LDLR activity in acute leukemia patients and the influence of HMG-CoA reduct ase inhibiter atorvastatin on leukemic cell proliferation were detected by MTT method. HMG-CoA reductase activities of leukemic cells were analysed by ultraviolet spectrophotometry.

用四氮甲唑蓝比色法检测AL患者的细胞生长抑制率、LDLR活性、HMG-CoA还原酶抑制剂阿托伐他汀对白血病细胞增殖作用的影响；紫外分光光度法测定白血病细胞HMG-CoA还原酶活性。

The effect of soybean hypocotyl extract on sucrase and maltase activity of rat intestinal mucosa was investigatedby colorimetric method, and glucose uptake into brush border membrane vehicles were determined by a rapid filtration method.

在体外比色法观察大豆胚轴提取物对大鼠小肠黏膜蔗糖酶和麦芽糖酶活性的影响，并用快速过滤法观察其对兔小肠刷状缘囊泡葡萄糖转运活性的影响。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。