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比色法

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objectiveto analyze the total content of flavonoids in nigella glandulifera preyn et sint by colorimetric method.methodsrutin was used as reference substance and the detection wavelength was 500 nm.

目的采用比色法测定维吾尔药黑种草子中总黄酮的含量。方法以芦丁作对照品,以亚硝酸钠-硝酸铝-氢氧化钠体系为显色剂,检测波长为500 nm。

objectiveto predict the promoting effect of chinese medicine on proliferation of rat bone marrow mesenchymal stem cells by bioactivity fingerprint in vitro.methodsthe ointment of plastrum testudinis was extracted by petroleum ether,ether and dichloromethane in burn to gain the extracts.the effects on the proliferation of bmmscs were examined by mtt assay and flow cytometry analysis, and each sample was studied by gc-ms.

目的运用生物活性指纹图谱预测其它中药能否促进骨髓间充质干细胞(bone marrow mesenchymal stem cells,bmmscs)。方法龟版浸膏依次用石油醚、乙醚、二氯甲烷提取,得到溶剂提取物,用mtt 比色法及流式细胞仪器研究了溶剂提取物干细胞活性,采用gc-ms 技术研究每一个提取物,根据总离子流色谱图的相同保留时间和相应峰面积,获得龟版浸膏 gc-ms 生物活性指纹图谱。

METHODS Acetylacetone, 4-Dimethylaminobenzaldehyd and alcohol without aldehyde were used as color reagents. The determination method was colorimetry.

以乙酰丙酮、无醛乙醇和对二甲氨基苯甲醛溶液为显色剂,用比色法测定。

Methods Acid dye colorimetry was used in the determination with bromocresol green buffer solution as the color developing agent with absorbance was determined at the detection wavelength of 417 nm.

采用酸性染料比色法,以溴甲酚绿缓冲液作为生物碱的显色剂,测定样品溶液在417 nm波长处的吸收度。

Methods Acid dye colorimetry was used in the determination with bromocresol green buffer solution as the color developing agent with absorbance was determined at the detection wavelength of 417 nm.

目的 建立脑脉通有效部位中总生物碱的含量测定方法。方法采用酸性染料比色法,以溴甲酚绿缓冲液作为生物碱的显色剂,测定样品溶液在417 nm波长处的吸收度。

Guangdong college of pharmacy,guangzhou,guangdong 510006,china;henan university of traditional chinese medicine,zhengzhou,henan 450008,chinaabstract:objective to establish a method for the determination of the content of total alkaloid in nao mai tong recipe.methods acid dye colorimetry was used in the determination with bromocresol green buffer solution as the color developing agent with absorbance was determined at the detection wavelength of 417 nm.results the calibration curve of the absorbance was linear with the concentration of ligustrazine in the range of 0.031~0.153 mg/ml(r=0.9998).the average recovery of ligustrazine was 100.95%,rsd=1.75%.

作者单位:广东药学院中药化学与分析教研室,广东广州 510006;河南中医学院,河南郑州 450008 目的建立脑脉通有效部位中总生物碱的含量测定方法。方法采用酸性染料比色法,以溴甲酚绿缓冲液作为生物碱的显色剂,测定样品溶液在417 nm波长处的吸收度。结果对照品川芎嗪质量浓度在0.031~0.153 mg/ml的范围内与吸光度呈良好的线性关系,r=0.9998。平均回收率为100.95%,rsd为1.75%。精密度和稳定性良好。

MethodsIn the solvent of 1 mol/L NaOH,using the reaction of the total components of water-soluble phenolic acidity in Danshen and the NaNO2-Al(NO3)3,the total components of water-soluble phenolic acidity in compound Danshen preparation(calculated with protocatechualdehyde )were determined by colorimetric spectrophotometry.

方法利用丹参中水溶性酚酸类成分与NaNO2-Al(NO3)3在碱性条件下的显色反应,比色法测定复方丹参制剂中水溶性酚酸的总量。

MethodsIn the solvent of 1 mol/L NaOH, using the reaction of the water-soluble phenolic acidity in Radix Salviae Miltiorrhizae and the NaNO_2-Al(NO_3)_3, the total components of water-soluble phenolic acidity in Radix Salviae Miltiorrhizae injections and drip solution(calculated with protocatechualdehyde) were determined by colorimetric s...

方法利用丹参中水溶性酚酸类成分与亚硝酸钠和硝酸铝溶液在碱性条件下的显色反应,比色法测定该两种制剂中水溶性酚酸的总量。结果原儿茶醛在3.336~14.46mg/L范围内呈良好的线性关系,回归方程为:A=0.02777C+0.01279(r=0.9996),加样平均回收率为100.8%,RSD为0.96%。结论该法简便快速,结果准确,重现性好,可用于该制剂的质量控制。

Note that the overall reaction for this fermentation cannot involve the consumption of nicotin -amide coenzymes or citric acid cycle intermediates.

标明这个发酵的所有反应不包括尼克酰胺辅酶或者三羧酸循环中间产物的消耗。比色法涉及氟离子对预先形成的颜色的消色作用。

The feasibility of determining the pentosan in pre-extraction liquor from eucalyptus chips by using Douglas colorimetric method was discussed in this paper. The visible spectrums of xylose, glucose, mannose, galactose and kraft lignin were compared under the same color reaction conditions. The results showed that the disturbance from hexoses and lignin can be eliminated when the pentosan is identified and determined by the maximal absorption at 553nm.

摘 要:探讨了 Douglas 比色法用于测定桉木半纤维素预提取液中戊聚糖的可行性,通过对木糖、葡萄糖、甘露糖、半乳糖和木素在相同条件下显色反应后的谱图进行比较,证明利用553 nm 处的最大吸收进行测定可以排除己糖和木素的干扰。

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