比色法

A method for the simultaneously quantitative determination of contents of aminopyrine and antipyrine by high-performance liquid chromatography was established.

建立高效液相色谱法测定安痛定注射液中氨基比林和安替比林的含量。

Josephus speaks of the Essenes as a sect which had existed in the time of the Maccabees, contemporaneously with the Pharisees and Sadducees, and which teaches that all things are determined by destiny, and that nothing befalls men which has not been foreordained; whereas the Pharisees make allowance for free will, and the Sadducees deny destiny altogether.

，约瑟夫谈到爱色尼节作为一个已存在的时间的马加比， contemporaneously与法利赛和撒都该人，并教导我们，一切事物都是确定的命运，并没有什麼befalls男子尚未注定；而法利使津贴的自由意志，并撒都该人完全否定的命运。

Are not Abana and Pharpar, the rivers of Damascus, better than any of the waters of Israel?

大马色的河亚罢拿和法珥法岂不比以色列的一切水更好吗？

Methods CNP absorbing MMC experiment was designed by geometric proportion method, while CNP-MMC desorbing MMC experiment was by diluting on 2 times. By the way of determining the free MMC concentration of the separating medium by pressurized liquor chromatography, we can get absorbing and desorbing rate.

采用等比法设计CNP吸附MMC实验，以2方式稀释CNP-MMC溶液设计CNP解吸附MMC实验，应用高压液相色谱法测定分离液中游离的MMC浓度，计算吸附率和解吸附率。

A method for the determination of residual pentane content in de-oiled asphalt of Liaohe heavy crude vacuum residuum was studied by using static headspace gas chromatography. The effects of gas-liquid phase ratio, equilibrium time and equilibrium temperature on headspace analysis were investigated.

采用静态顶空气相色谱法测定辽河稠油减压渣油高软化点脱油沥青中戊烷的含量，确定了色谱的测定条件，研究了气液相比、平衡时间、平衡温度等因素对定量分析的影响。

An efficient method for analyzing Arbutin and Hydroquinone in cosmetics by reversed-phase high performance liquid chromatography is developed.

探讨了高效液相色谱法同时测定美白化妆品中熊果苷和氢醌含量的方法，先对样品进行预处理，处理液为氯仿-饱和氯化钠溶液(体积比2∶1)，再选用Apollo C18柱，以甲醇-磷酸缓冲液-冰醋酸(体积比25∶475∶1)混合液为流动相，紫外检测，波长为254nm。

The UV and IR spectroscopic studies of the monomer films showed that the photoreaction ratio of monomer (about 50%) was higher than the corresponding photosensitive polymer (about 10%, PVCi). The polarized IR spectra proved that obvious anisotropic absorption was displayed in the irradiated photosensitive film. With polarizing microscopy method, we found that these kinds of photoalignment films exhibited good alignment ability, and the stability of the photoalignment film could reach about 100℃. 2 A photosensitive self-assembled monolayer was fabricated that contained photo-cycloadditable groups, such as, coumarin and cinnamate. The reaction process were detected by contact angle method because the IR absorption of monolayer was very weak. The photosensitive self-assembled monolayer promote uniform alignment of nematic liquid crystals by LPUV, and it thermal stability could reach about 130℃. 3 A cationic bolaform amphiphiles was synthesized that had two photosensitive double bonds, and can be polymerized by ultraviolet. A layer-by-layer self-assembled deposition film was prepared in aqueous solution by using this material and poly (sodium 4-styrenesulfonate). The UV-Vis. spectra proved that the multilayers film was uniform and layer-by-layer. When LPUV irradiated the film, the photopolymerizable double bond underwent [2+2] cycloaddition, and the production of the reaction distributed along the polarized direction of incident LPUV. Polarized UV-Vis. spectra indicated that the irradiated self-assembled multiplayer film was anisotropic, and the dichromic ration is about 0.09, it is higher than the spin-coating method of photosensitive polymer or monomer. We used this anisotropic film as alignment layer of nematic liquid crystals, and obtained a uniform and stability alignment result, the static contrast ratio is about 100, which is very close to application of liquid crystal device. This thesis is an instance that the photochemical material was used in the physics region of liquid crystals, the route that was from photosensitive monomer, self-assembled monolayer film to self-assembled multiplayer film was undergone, by this logical research method photoailgnment of liquid crystal was extended and consummated.

围绕上述思路开展了如下工作：1合成了三种可进行光二聚反应的光敏小分子单体，LPUV 辐照制作了光控取向膜；用紫外-可见光谱法和红外光谱法分析了薄膜中的光化学反应，发现小分子的光反应程度可达到50%以上，至少比光敏高分子高出5倍；薄膜的偏振红外光谱证实，经过LPUV 辐照后薄膜出现了明显的各向异性；将小分子光控取向膜制成液晶器件，在偏光显微镜下观察看到均匀一致的液晶取向；并且热稳定性达到100℃左右，接近实用化要求。2制备了含肉桂酸、香豆素基团的自组装单层膜；采用接触角的方法监测自组装反应的动力学过程；紫外-可见光谱证实了自组装单层膜在石英基板表面形成；自组装单层膜经LPUV 辐照后能诱导液晶均匀取向，而且热稳定性可达130℃，热稳定性进一步提高。3合成了含有光敏双键的双季铵盐，在水溶液状态下将它和聚乙烯基苯磺酸钠沉积生成layer-by-layer 自组装多层膜，紫外-可见光谱证实这是一个逐层均匀的沉积；在LPUV 照射下，自组装膜中的光敏双键按照偏振方向发生[2+2]环加成反应，形成取向膜，用二向色性值评价的分子取向度高于高分子及小分子光控取向膜的水平；实验证明用自组装多层膜制成的液晶器件亮暗态均匀，静态对比度约为100，达到实用化器件的水平。

BQJ-34 was cultured on the biotin fermentation media for 48 hour ,a 10ml fluid medium was boiled for an hour and was centrifuged (10,000r/min , 30 min), the supernatant is filleted with the 0.22μm filters and the biotin content of filter liquid is detected by RP-HPLC based on the biological samples regression analysis, recovery, accuracy testing.

利用生物素培养基对菌株BQJ-34进行振荡培养，取48h的细菌培养液10ml置100℃条件下水浴1 h，随后在超速离心机上以10 000 r/min，30 min离心，取上清液用0.22μm滤膜过滤，用高效液相检测滤液中的生物素含量，并通过对生物素样品的回归分析和回收率、精密度试验，最终确定利用反相高效液相色谱法可用于培养液中生物素测定，其最佳检测条件是：用安捷伦Zorbax SB-C18(4.6~※250cm，5μm)色谱柱，以K_2HPO_4(0.04mol，pH2.5)和CH_3CN(二者体积比为91.5：8.5)为流动相，紫外检测波长为215nm。

By this method, the productivity of 3. 18g/l extracellular polysaccharides was obtained. The optimum extraction method was obtained by RSA. It was determined as follows: 91. 4 ℃, 2. 9h, the weight ratio between mycelia and water was 1 to 3. The productivity was 12. 38% of dry mycelia. Set the purifying methods of Grifola frondosa: Precipitated polysaccharides part by 60% ethanol→removed protein by Sevag method→removed coloring matter by H〓O〓→removed salts by dialyse→DEAE-cellulose column chromatogram→ purified polysaccharides groups.

对于胞内多糖的提取，采用湿菌体经捣碎再高压破壁的方式破碎菌体细胞，采用响应面分析法得出优化后的提取条件为：提取温度91.4℃，提取时间2.9h，料水比1：3，此提取条件下胞内粗多糖的率为干菌体重的12.38％建立了灰树花多糖分离纯化的技术路线 60％乙醇沉淀的粗多糖→Sevag法脱蛋白→H〓O〓脱色→透析脱盐→DEAE-纤维素层析分离→多糖组分。

A simple,rapid,sensitive and accurate RP HPLC for simultaneous determination of ketamine, phenobarbitone and atropine in plasma was established.

本文采用ZorbaxC8色谱柱，甲醇－磷酸盐缓冲液（69∶31）为流动相，220nm为检测波长，非那西丁为内标，建立了同时测定人血浆中氯胺酮、苯巴比妥和阿托品浓度的反相高效液相色谱法。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。