比色法

Methods MTT colorimetric assay was used to test in vitro cytotoxicities of the chemotherapeutics against human hepatoma cell lines.

采用MTT比色分析法测定多柔比星或米托蒽醌对体外培养的4株人肝癌细胞的细胞毒作用。

Methods Serum level of AFU,AFP was measured by chemistry speed colorimetry and corpuscule enzymeimmunoassay in 88 patients with primary hepatic carcinoma,56 patients with benign disease and 68 normal subjects.

方法采用化学比色速率法和微粒子酶法，测定88例原发性肝癌患者、56例肝良性疾病患者血清AFU和AFP水平，并与68例正常对照组进行比较。

The wastewater from gum benzion production contains benzaldehyde, cyanide, gum benzoin and ethyl benzoate. When the cyanide is determined by isonicotinic acidpyrazalon method, the ethyl benzoate is distilled and goes into NaOH absorption liquid with cyanide in the process of predistillation, because its boiling point is low. Thus the absorption liquid produces a great deal of foam and feculence.

摘要］有机化工产品安息香生产废水中含有苯甲醛、氰化物、安息香和苯甲酸乙酯，在用异烟酸吡唑啉酮法对其进行氰化物测定时，由于油酯类的沸点较低，在预蒸馏过程中被蒸出和氰化物一起进入NaOH吸收液，使得吸收液产生大量泡沫和沉淀，严重干扰后续比色测定。

Fund Project: the National Natural Science and Technology Source Program, No. 2001DEA1006*Abstract: Number of neural stem cells is small. NSCs look like circle or ellipse with or without short neurite, with large nuclear-cytoplasmic ratio and deep karyotin. NSCs have no visible differences with other kinds of cells in appearance, and have no cell surface marker. Currently, there are mainly three aspects to identify NSCs: expression of specific nerve antigen, including nestin, Musashi, transcription factor, and cell adhesion molecules; self-renewal ability including single cell clone analysis, BrdU mark and S phase cell; the potential of multi-direction differentiation including immunocytochemical process and reverse transcriptase polymerase chain reaction.

由于神经干细胞的数量很少，从细胞形态来看神经干细胞为圆形或椭圆形，无或有较短的突起，核质比大，核染色较深，形态上与其它种类的细胞没有明显的差异，并且未找到一种细胞表面特异性标志物，因此目前鉴定神经干细胞主要有以下3个方面：特异性神经抗原的表达，包括巢蛋白、Musashi、转录因子及细胞黏附分子；自我更新能力，包括单细胞克隆分析、BrdU标记S期细胞；多向分化潜能，包括免疫细胞化学法、聚合酶链反应色法。

Number of neural stem cells is small. NSCs look like circle or ellipse with or without short neurite, with large nuclear-cytoplasmic ratio and deep karyotin. NSCs have no visible differences with other kinds of cells in appearance, and have cell surface marker. Currently, there are mainly three aspects to identify NSCs: expression of specific nerve antigen, including nestin, Musashi, transcription factor, and cell adhesion molecules; self-renewal ability including single cell clone analysis, BrdU mark and S phase cell; the potential of multi-direction differentiation including immunocytochemical process and reverse transcriptase polymerase chain reaction.

由于神经干细胞的数量很少，从细胞形态来看神经干细胞为圆形或椭圆形，无或有较短的突起，核质比大，核染色较深，形态上与其它种类的细胞没有明显的差异，并且未找到一种细胞表面特异性标志物，因此目前鉴定神经干细胞主要有以下3个方面：特异性神经抗原的表达，包括巢蛋白、Musashi、转录因子及细胞黏附分子；自我更新能力，包括单细胞克隆分析、BrdU标记S期细胞；多向分化潜能，包括免疫细胞化学法、聚合酶链反应色法。

Two methods, including high performance gel permeation chromatography and gelatin nephelometry,are used to investigate the stability of marine sulfated polysaccharide 916 by lysozyme and trypsin, and weight average molecular, molecular weight distribution and dissociative sulfate content of 916 are determined.

利用高效凝胶渗透色谱法和明胶比浊法研究了海洋硫酸多糖 916在溶菌酶和胰蛋白酶作用下的稳定性，分别考察了酶作用下 916的重均分子量、分子量分布系数和游离硫酸根含量的变化。

Two methods including high performance gel permeation chromatography and gelatin nephelometry were used to test the stability of 916 by lysozyme and trypsin.

在稳定性研究方面，选择生物体液中存在的两种酶---溶菌酶和胰蛋白酶作为考察条件，采用高效凝胶渗透色谱法和明胶比浊法，分别考察不同指标，从分子量及分子骨架是否变化到有效基团是否脱落以确定916的稳定性。

In order to explore relationship between pathological classifications and humoral auto immune status, the serum,cerebrospinal fluid and nucleus pulposus of 28 disc herniation patients were examined for the production of IgG and IgM by rate nephelometry and immunohistochemical protocol.

为探讨腰椎间盘突出症的不同病理分型和自身体液免疫状态之间的相互关系，采用速率散色比浊法和免疫组化法对28 例腰椎间盘突出症患者血清、脑脊液和髓核组织中IgG、IgM 进行测定。

Water, methanol and ethyl acetate extraction method were used to extract non-oil components from Myrtus communis Linn. leaves, and then radical scavenging activity and antioxidant role of these extracts were investigated using four chemiluminescence systems and two colorimetric systems.

采用水提取法、甲醇－水提取法和乙酸乙酯提取法3种方法从香桃木叶中提取非精油组分，然后用4种化学发光体系和2种比色体系检测其清除自由基和抗氧化作用。

Methods The plasma NO was measured in 40 elderly patients with CHF by colorimetric analysis and SOD activity by the autoxibation of pyrogallic acid.

方法采用比色分析法和邻苯三酚自氧化法测定了40例老年CHF患者的血浆NO浓度及SOD活性。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。