比色法

An electrophotometric method has been worked out for the determination of magnesium in cast iron using titan yellow as indicator.

本文报告将地丹黄比色法应用於测定铸铁中之镁的试验结果。

The content of NaCl in soda ash is determined by colourimetry of mercury thiocyanate.

作者采用硫氰酸汞比色法测定纯碱中氯化钠的含量。

In order to study the effects of pre-heating on the sensitivity of cells to tumor necrosis factor-α, the K562 cells were pre-heated at 40℃ for different times to induce heat shock protein 70(HSP70). To observe the effects of TNF-α concentration on the sensitivity of pre-heated cells, cells were pre-heated for 60 min and treated with TNF-α(50,100,200 and 400U/ml) for 16 hours, and the cells vitalities were detected by MTT.

为探讨预热对肿瘤细胞肿瘤坏死因子α敏感性的影响及其机理，将白血病患者胸腔渗出细胞系(K562细胞)在40℃预热不同时间(0、30、60、90、120、150和180min)，使细胞热应激蛋白70(HSP70)高表达；然后将预热60min细胞和未预热细胞分别暴露于50、100、200和400U/ml的TNF-α16小时，用MTT比色法检测细胞活力。

Results:Total glucosides of paeony in concentration of 0.8～25.6 mg/L demonstrated marked dose-dependent inhibitory effect on fibroblast- like synoviocytes proliferation.

利用MTT比色法测定不同浓度的甲氨蝶呤和白芍总苷单独或联合作用于滑膜细胞，对滑膜细胞增殖的影响。

Introduces the method to extract total saponin in sisal hemp with supercritical CO2,and determ ination of total saponin by vanillin colorimetry.

采用超临界CO2萃取法萃取剑麻中的总皂苷，用香草醛比色法测定萃取产物中总皂苷。

CoCl2 (a chemical hypoxia-mimetic agent) was used to establish the chemical hypoxia-induced PC12 cell injuries model. Sodium hydrosulfide was used as a H2S donor. The viability of PC12 cells was measured by CCK-8 assay. The percentage of apoptotic cells was assessed by propidium iodide stain flow cytometry. The morphological change of apoptotic cells was tested by using the chromatin dye Hoechst 33258. The mitochondrial membrane potential was analyzed by rhodamine 123 staining and photofluorography. The level of reactive oxygen species in PC12 cells was measured by DCFH-DA staining and photofluorography.

应用化学性缺氧模拟剂CoCl2在PC12细胞建立化学性缺氧损伤模型；以硫氢化钠作为H2S的供体；应用CCK-8比色法检测细胞存活率；碘化丙啶染色流式细胞技术检测细胞凋亡率；Hoechst33258染色检测细胞凋亡的形态学变化；罗丹明123(Rh123)染色荧光显微镜照相检测细胞线粒体膜电位；2'，7'-二氯荧光黄双乙酸盐染色荧光显微镜照相检测细胞内的活性氧水平。

Methods Male SD rats were given drinking water containing manganese chloride (MnCl24H2O 0.5g/L and 5.0g/L respectively) for 60 days. At the end of experiment, all the rats of the manganese-exposed groups and control group were decapitated after anaesthesia with 2% sodium phenobarbitone intraperitoneally, the brains were removed and activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphate, cholinestarase, monoamine oxidase, superoxide dismutase, nitric oxide synthase, and levels of glutathione, maleic dialdehyde, and proteins were biochemically analyzed.

大鼠经饮水连续染锰（MnCl24H2O 0.5g/L和5.0g/L）60d后，应用荧光光度比色法测定新鲜脑组织匀浆的谷丙转氨酶、谷草转氨酶、碱性磷酸酶、胆碱酯酶、单胺氧化酶、氧化亚氮合酶、超氧化物歧化酶活性和谷胱甘肽、丙二醛、蛋白质含量，并设生理盐水对照组。

The content of hydroxyproline can be determined by colorimetry ,and the content of hydrolyzed animal collagen proteins in adulterated milk was calculated by Hyp content.

采用比色法快速定量检测乳中掺加的动物胶原水解蛋白。

Methods TIL in the patients with PHC were separated with combinative enzymatic digestion of type Ⅰ,Ⅳ collagenases,type Ⅴ hyaluronidase and type Ⅰ deoxyribonuclease followed by discontinuous density gradients.Cytotoxicity of TIL to liver cancer cells was detected with MTT method.

采用Ⅰ型和Ⅳ型胶原酶、Ⅴ型透明质酸酶和Ⅰ型DNA酶四联酶消化，应用不连续密度梯度离心分离TIL，采用MTT比色法检测TIL对自体癌细胞及肝癌细胞株的细胞毒。

Chromatometry was respectively used to assay activity of ChAT and AChE in cerebral cortex、hippocampus and corpus striatum.

用比色法测定大鼠皮层、海马和纹状体区ChAT与AChE的活性。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。