比色法

Andrographis paniculata;Total localities;colorimetry;Content determination

穿心莲；总内酯；比色法；含量测定

Control group was only given culture fluid.

采用MTT比色法测定细胞生长抑制率。

ConclusionIt is the first time that uniform design is used to optimize extraction conditions of polysaccharide. The extraction rate of polysaccharide is high. Phenol-vitriolic colorimetry was a stable and reproduciable method to determine content of polysaccharide in Fructus Corni.

结论首次运用均匀设计优化山茱萸多糖的提取条件，提取得率高，苯酚-硫酸比色法测定山茱萸多糖含量稳定性、回收率较好。

MethodsThe effect of curcumine on the proliferation of spleen lymphocytes through three different administration routes were determined with MTT test.

方法通过噻唑蓝比色法比较姜黄素3种给药途径对小鼠脾淋巴细胞增殖的影响。

ObjectiveTo probe into the hyperplasy effect of different curcumine administered approaches for the splenic lymphocyte of mice.

目的探讨姜黄素3种不同给药途径对小鼠脾淋巴细胞增殖的影响。方法通过噻唑蓝比色法比较姜黄素3种给药途径对小鼠脾淋巴细胞增殖的影响。

Out-of-body experience certified curcumine could suppress the hyperplasy of the mice splenic lymphocyte.

方法通过噻唑蓝比色法比较姜黄素3种给药途径对小鼠脾淋巴细胞增殖的影响。

Methods Human monocyte cell line THP-1 was cultured and induced to macrophage by PMA treatment. The effect of 9-cisRA on the differentiation induced by PMA was studied. Cell morphology was observed by phase contrast microscope and the adhesion rate of THP-1 was detected by methyl thiazolyltetrazolium assay. The cell surface markers involved in differentiation of mono cyte/macmphage (CD11b, CD36) were analyzed by flow cytometry.

体外培养人单核细胞系THP-1，经佛波酯诱导分化为巨噬细胞，同时给予9-顺式维甲酸对佛波酯诱导分化进行干预，通过相差显微镜观察细胞形态，MTT比色法检测细胞贴壁率，流式细胞仪检测与单核／巨噬细胞分化有关的细胞表面标志物CD11b和CD36的表达。

Synoviocytes were incubated with different concentration of methotrexate and total glucosides of paeony respectively or combined.

利用mtt比色法测定不同浓度的甲氨蝶呤和白芍总苷单独或联合作用于滑膜细胞，对滑膜细胞增殖的影响。

So it is concluded that the BP had immunotoxicity effects on haemocytes and the neutral red assay could be used as a sensitive index to evaluate the effect of B P on the haemocyte viability of scallop C. farreri.

由此可见，BP对栉孔扇贝血细胞活性具有免疫毒性作用，中性红比色法可以作为评价BP对栉孔扇贝血细胞免疫活性的敏感指标。

In BP exposure experiment, except that the neutral red assay showed that BP significantly inhibited the haemocyte viability, the other methods did not detect the inhibition effect, besides, the results of the neutral red assay showed that the inhibition degree had a positive correlation with the concentration and exposure time of BP.

采用中性红比色法检测BP对栉孔扇贝血细胞活性具有显著抑制作用，且抑制程度与BP作用浓度、时间呈正相关，其余方法均未检测出BP对血细胞活性的影响。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。