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Results At different time and concentration,genistein had different effects on osteoblastic cell proliferation.

用四甲基偶氮噻唑蓝比色法检测细胞增殖,碱性磷酸酶活性测定检测细胞的分化。

Fungal elicitors were extracted from Colletorichum lagnarinm, Phoma filtrate, Fusarium oxysponum, Asperillus niger and culture with HRPG. The total ginseng sponin and four kinds of monomeric sponins were analysed by UV spectrophotometry and RP HPLC.

提取黄瓜炭疽病菌 Colletorichumlagnarinm 、青菜炭疽病菌 Phomafiltrate 、棉花枯萎病菌 Fusariumoxysponum 、黑曲霉 Asperillusniger 中的真菌诱导子与人参毛状根共培养,应用HPLC及比色法对培养物中总苷及几种单体皂苷进行分析测定。

The species distribution and transformation characteristics of polysilicic acid in the presence of acetic acid were investigated by chemical Si-Mo assay analysis.

针对聚硅酸金属盐絮凝剂因硅酸聚合胶凝而失活的问题,依照Si-Mo比色法的原理,对乙酸存在时的聚硅酸形态及其转化规律进行研究。

In addition, output of urine calcium and sodium in DM rats is decreased,and reabsorption function of renal tubule is increased.

采用微量溶菌酶比色法测定反映肾小管的重吸收功能的溶菌酶含量。

Methods Compounds were isolated and purified by various column chromatographic techniques and their structures were elucidated on the basis of spectral analysis (IR, ESIMS, 1HNMR, 13CNMR). The inhibitory activity against the proliferation of HL60, REH and Raji cell lines was determined by using MTT assay.

用色谱方法分离栀子中化学成分,用波谱技术(IR,ESIMS,1HNMR,13CNMR)进行结构鉴定,用四唑盐比色法测定化合物对白血病细胞(HL60人早幼粒细胞白血病、REH人急性淋巴细胞白血病、 Raji淋巴瘤)增殖的影响。

Methods Forty-eight healthy black and blue rabbits were chosen and divided into 6 groups at randomly. Experimental groups were performed surgery with CO2 laser sclerectomy in both eyes. Contents of nitric oxide and malonyldialohyed and the activity of Na(superscript +)-K(superscript +)-ATPase were tested with biochemical assays.

健康青紫兰兔48只,随机分为6组,实验组动物双眼均接受CO2激光外路巩膜切除术,化学比色法测定视网膜匀浆中一氧化氮、丙二醛的含量和Na-K-ATP酶的活性,进行统计学分析。

Methods : MTr assay was used to examine the effect of Sorbaria sorbifolia on the proliferation of HepG-2 cell and flow cytometry was used for the cell cycle distribution.

采用MTT比色法观察珍珠梅提取物抑制肝癌HepG-2细胞增殖;采用流式细胞仪检测其对HepG-2细胞周期分布的影响,同时结合倒置显微镜、HE染色、透射电镜及免疫细胞化学方法检测凋亡相关基因bcl-2、p53的蛋白表达,观察其诱导肝癌HepG-2细胞凋亡的作用。

The changes in the weight, exponential, structure of the tissue, cell apoptosis in testis and epididymis and the changes in the sperm tectology in epididymis were measured on the day 8, 13, 21 and 35 of the treatment,respectively,by the method of morphological examination.

分别在热应激持续到8d、13d、21d和35d时,应用形态学观察法,研究受试鼠睾丸和附睾重量及指数、组织结构、细胞凋亡和附睾精子形态学的变化;应用免疫荧光细胞化学和蛋白质印迹分析的方法,研究睾丸和附睾组织中热休克蛋白70(HSP70)的表达;应用比色法检测睾丸和附睾组织内活性氧和丙二醛、抗氧化物酶(SOD、GSH-Px、CAT)的活力或浓度的变化;应用交配试验,检测雄鼠受精能力及其致孕雌鼠的产仔数的变化,用以揭示热应激对雄鼠生殖机能的影响。

MethodsThe alkaloid was extracted by chlorolform from Pinellia ternate and tested at 417nm with the spectroscopy by acid dye.

方法采用氯仿提取,依据酸性染料比色法的原理,在417 nm波长下测定,并作方差分析。

In the determination of oil colour,F Lovbind tintometer is greatly influenced by operator's subjectivity and environment.

在油脂色泽测定中,罗维朋目视比色法受操作者的主观性和周围环境影响较大,需要一种客观测定的仪器来代替,以消除由于主观性而产生的误差。

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