比色法

It presents the mechanism to Abstract total cyanide by using phosphoric acid-EDTA and the conditions to measure cyanide by using isonicotinic acid-pyrazolone colorimetry.

阐述了用磷酸———EDTA提取总氰化物的机理以及用异烟酸———呲唑啉酮比色法测定氰化物的条件。

Methods The cytotoxic effects of the four kind extracts of Livistona chinesis on two tumor cell lines cultured in vitro were tested by MTT assay.

采用四甲基偶氮唑盐比色法，观察蒲葵子提取物的各萃取部位对肝癌细胞HepG2和白血病细胞HL60增殖的抑制作用。

To explore the clinical significance of combined detection of serum CYFRA21-1,CA153 and TSGF levels for diagnosis of mastocarcinoma.

目的探讨血清CYFRA21-1、CA153、TSGF联合检测对乳腺癌诊断的意义。方法采用电化学发光检测CYFRA21-1、CA153，化学比色法测定TSGF。

MTS/PMS microcolorimetry was applied to assess A490 absorption factor of HASMCs cell. In all groups, cell cycle distribution was detected by flowcytometry.

MTS/PMS微量比色法检测各组HASMCs细胞A490吸光度，流式细胞仪检测细胞周期分布。

MethodsAfter the conversion coefficient of Moringa oleifera polysaccharides to glucose was obtained, contents were determined by anthrone-H2SO4 colorimetry.

采用精制辣木叶多糖测得辣木叶多糖对葡萄糖的换算因子后，采用蒽酮-硫酸比色法测定，测定波长为620 nm。

Methods: The inhibitation of HL-60 cells by Rapamycin was measured by MTT assay. Morphic change of the apoptosis of HL-60 cells was observed by inverted microscope and light microscope, trapezoid straps by DNA agarose gel electrophoresis, and apoptosis rate by flow cytometry. Western-blot analysis was performed to detect the expression of Bax and Bcl-2 proteins after rapamycin treatment.

用MTT比色法测定雷帕霉素对HL-60细胞增殖的影响；用倒置显微镜和荧光显微镜观测细胞凋亡的形态学改变；DNA琼脂糖凝胶电泳观测凋亡的梯形条带；流式细胞仪检测细胞凋亡率；Western-blot检测HL-60细胞经雷帕霉素处理后，Bax、Bcl-2蛋白的表达情况。

The contents of polysaccharide in leaves of Morus alba L.

用蒽酮-硫酸比色法测定了桑叶中多糖的含量。

Methods According to Chinese standard analysis method of foods (GB/T 5009133-2003), 108 samples of cured meat products were determined nitrite content by naphthyl ethylenediamine hydrochloride colorimetry. The results were analyzed.

采用国家食品卫生检验标准方法(GB/T 5009133-2003)即食品中亚硝酸盐与硝酸盐的盐酸蔡乙二胺比色法对市场上抽检108份腌腊制品中亚硝酸盐含量进行测定，对测定结果进行分析。

Concentrations of water soluble phenolic substances in the leaves of male and female trees of Acer negundo L.were successively determined within an entire growing season by method of iron tartrate colorimetric analysis.

采用酒石酸铁比色法对复叶槭雌、雄株叶片中水溶性酚类物质的含量进行了一个生长周期的跟踪测定，对不同性别、不同年龄、不同光照条件下的复叶槭植株叶片中水溶性酚类物质的含量做了比较分析，同时研究了叶片中叶绿素的含量与水溶性酚类物质含量之间的关系。

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。