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比色法

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MethodsThe content was determined by solvent extraction and acid dye colorimetiy, with Acanthoine Ruscope-ine (C16H22N4O2) as a control criterion and bromocresol green as the acid dye, and the wavelength was at 418 nm.

方法采用溶剂萃取酸性染料比色法测定,以去氢飞廉碱(C16H22N4O2)为对照品,溴甲酚绿为酸性染料,用氯仿萃取,检测波长418 nm。

METHODS Acidic dye colorimetry with bromothymol blue as ion pair reagent was employed to determine the alkaloids in samples.

用溴麝香草酚蓝同川芎生物碱形成稳定的有色离子对酸性染料比色法测定总生物碱的含量。

Based on the absorbance change of indicators with the concentration of hydrogen ion released from the enzyme-catalyzed reaction, a convenient colorimetry method is established for the assay of acidic phospholipase 〓 and glycogen phosphorylase b Brilliant yellow and bromothymol blue are chosen as indicators for assays of acidic phospholipase 〓 and glycogen phosphorylase b by following the absorbance changes at 495 nm and 615nm respectively The method is simple, sample-saving, sensitive and valid for a wide range of enzyme concentrations.

为了研究糖原磷酸化酶的激活动力学和酸性磷脂酶〓的复性过程,我们根据酶催化反应中释放氢离子浓度的变化引起相应的指示剂的光吸收发生变化的原理,建立了一种简捷的比色法,用于测定酸性磷脂酶〓和糖原磷酸化酶b的活性。选用亮黄和溴麝香草酚兰分别作为酸性磷脂酶〓和糖原磷酸化酶b测活的指示剂,在495 nm和615 nm处检测二者的光吸收值的变化,可以测定酶活。本方法的优点是,可在比较宽的酶浓度范围内进行活力测定,而且操作简单、节省样品、灵敏度高。

Method8: The paPef, fOr the first time, studied the apoptosis or necrosis of K562cclls induced by bungarus multicinclus crude venom and several components frOmcrude venom by cation exchange chromatography\' through the quatitative analysis offlow cytometry as well as the qualitative analysis, such as inversed opticalmicroscopy, MTT method and fluorescent microscopy, and so on.

本卖验采用羧甲基纤维素C-52阳离子交换层析将银环蛇粗毒进行了若干毒素组分的分离纯化,并以流式细胞术定量分析为主,以倒置显微镜、MTT比色法、荧光显微镜等多种定性分析为辅,首次对银环蛇粗毒及其若干组分蛋白对人慢性粒细胞白血病K562肿瘤细胞的细胞杀伤作用和凋亡诱导情况进行了研究。

The influence on physiques of Camponotus japonicus which were kept indoors in winter was studied. Their survival rate was counted. The activity of SOD and POD was tested by spectrophoto-meter.

研究室内过冬对日本弓背蚁生活力的影响,统计法测定了其存活力的变化,分光光度计比色法测定了野生蚁及低温处理蚁超氧化物歧化酶、过氧化物酶等指标。

A quantitative determination method of polyphenol from Canarium album Rauesch.

采用Folin Ciocalteu比色法研究了橄榄多酚的定量测定方法。

We have measured the serum levels of total cholic acid in 103 samples of obstructive jaundiced patients and 83 samples of gallbladder stone patients without jaundice by enzymeconjugated colorimetric analysis method.

采用酶偶联比色法测定47例(103例次)阻塞性黄疸和53例(83例次)无黄疸单纯胆囊结石患者血清总胆酸的水平,并探讨其与黄疸程度及肝细胞损害的关系。

A fast colorimetric method of determining fatty alcohol polyoxyethylene ether in compact laundry powder with cobaltic thiocyanate was discussed.

介绍了用硫氰酸钴盐比色法对浓缩粉中脂肪醇聚氧乙烯醚(AEO9)含量进行快速测定的方法,该法准确、方便,相对标准偏差小于 1%,浓缩粉中其他组分对测定结果无干

Development of any new colorimetric method should involve such a test procedure.

任何新的比色法的改进应该包括这样一种试验程序。

The colorimetric method is one of the important experiment technology in biochemistry, it is used widely in the determination of many substrates.

比色法是生物化学实验的重要技术之一,广泛应用于多种物质的定量测定。

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