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In addition, the expression of VEGF and MMP-2 were analyzed by western blot.

MTT比色法检测细胞对细胞外基质的粘附能力;5。

Positive high voltage prick electrostatic field whit different intensity function to the process of budding of soybean seed,measured Germination potential and Sprout ratio respectively at the second and third day of the bourgeon,measured dry weight of sprouts after drying;and Amylase activity measured by colorimetric method.

采用不同强度的正高压芒刺静电场作用于萌发过程中的大豆种子,在种子萌发的第2天和第3天分别测量其发芽数和芽长,烘干后称量芽干重,计算了其发芽势及发芽率,并用比色法测定了淀粉酶活力。

Methods The target DNA sequence of yrdC was obtained from human spleen tissue by using RT-PCR,construct high titer recombinant adenovirus Ad-yrdC by using AdEasy adenovirus carrier system,detect yrdC protein expression by using Western blot and immunohistochemical method,set up control group and transfected Ad-Null group,and study the effects Ad-yrdC on BGC-823 cell line by drawing cell growth curve and MTT chromatometry.

方法用RT-PCR方法获取人脾脏组织yrdC基因序列,采用AdEasy腺病毒载体系统构建携带yrdC基因的高滴度重组腺病毒Ad-yrdC,将其转染胃癌BGC-823细胞,应用Western blot和免疫组织化学法检测yrdC蛋白表达,设对照组和转染Ad-Null组,并分别绘制细胞生长曲线和MTT比色法研究Ad-yrdC对BGC-823细胞的影响。

Growth of cells were observed daily by MTT assay, the status of cell proliferation were observed by Plate colony formation, and the cell cycle of the cells were observed with flow cytometry .

应用MTT比色法绘制细胞生长曲线;克隆形成实验了解细胞增殖情况;流式细胞仪观察细胞周期。

Methods Cementum chips were obtained from diseased roots exposed to periodontal pocket.

扫描电镜下观察PDLC在根面上的生长状况,用噻唑蓝比色法分析细胞在根片上的附着和增殖。

Methods: The sensibility of 13 chemotherapeutics were measured in 13 cases of carcinoma of gallbladder and 32 cases of cholangiocarcinoma by MTT assay.

采用四氮唑比色法检测13例胆囊癌和32例胆管癌对13肿化疗药物的敏感性。

Methods The activity of urine NAG isoenzyme, NAG/Cr and blood NAG isoenzyme in 47 patients with acute CVD (15 patients with brain hemorrhage and 32 patients with brain infarction) were examined by p-nitrobenzene chronometry.

采用对硝基酚比色法,分别测定了47例急性脑血管疾病患者(其中脑出血15例,脑梗死32例)的尿液NAG同工酶谱、尿NAG与尿肌酐的比值、血清NAG活力。

Objective To evaluate relationship between urine NAG isoenzyme and acute cerebrovascular disease. Methods The activity of urine NAG isoenzyme, NAG/Cr and blood NAG isoenzyme in 47 patients with acute CVD (15 patients with brain hemorrhage and 32 patients with brain infarction) were examined by p-nitrobenzene chronometry.

目的 探讨尿液N-乙酰-β-D-氨基葡萄糖苷同工酶与急性脑血管病的关系方法采用对硝基酚比色法,分别测定了47例急性脑血管疾病患者(其中脑出血15例,脑梗死32例)的尿液NAG同工酶谱、尿NAG与尿肌酐的比值、血清NAG活力。

In this article, we studied some components in giant panda seminal plasma and their relationship with semen characteristics.The protein bands were separated by SDS-PAGE, platelet-activating factor acetylhydrolase activity and glutamic-aspartic aminotransferase activity were detected by ELASE and chymic colorimetry respectively. The results are as followed:Twelve protein bands in giant panda seminal plasma were identified by SDS-PAGE, the molecular weights were 74.9、59.6、 56.2、 54.7、 47.3、 38.2、 30.8、 23.8、 18.4、 16.8、14.0 and 13.4kDa respectively, six of them (74.9, 59.6, 56.2, 47.3, 30.8 and 13.4kDa) werepresent in all samples. However, there was no relationship was found between the abnormalprotein bands appeared in several samples and semen characteristics in our experiment.

本研究采用SDS-PAGE对大熊猫精浆蛋白电泳谱带特性进行分析,使用酶联免疫方法检测精浆血小板激活因子乙酰水解酶活性、化学比色法检测精浆谷草转氨酶活性,并初步探讨了这些物质与大熊猫精液质量的关系,结果显示:(1)大熊猫精浆SDS-PAGE电泳共分离得到12条蛋白谱带,其分子量分别为74.9kDa、59.6kDa、56.2kDa、54.7kDa、47.3kDa、38.2kDa、30.8kDa、23.8kDa、18.4kDa、16.8kDa、14.0kDa和13.4kDa,其中74.9kDa、59.6kDa、56.2kDa、47.3kDa、30.8kDa、13.4kDa谱带为所有个体大熊猫精浆样品所共有。

Its antioxidant effect was tested by the colorimeter and compared with Nectar. Hydroxyl .

并采用比色法测定其对·OH和O-2·的清除能力,并用甘露醇进行比较。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

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A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。