比色法

Methods The cultured 3T3 -L1 cells were pretreated with naringin, and then MTT method was used to detect the proliferation of the cells, oil red O staining method and spectrophotography were applied to analyze the degree of differentiation.

培养3T3-L1细胞，并用不同浓度的柚皮苷进行干预，以四甲基偶氮唑盐法检测细胞增殖，用油红O染色和染色比色法分析脂肪细胞的分化程度。

Extract and quantitative analysis of biological active ingredients total triterpene s in Suosuo grape.

采用正交实验设计，利用超声法从琐琐葡萄中提取生物活性成分总三萜，比色法测定总三萜的含量。

Two techniques were compared that uses the UV absorbance at 280nm of 0.5mol/L K_2SO_4 extracts of fumigated and unfumigated soils and fumigationwaterlogged incubation to estimate the concentrations of nitrogen in the SMB.

用氯仿熏蒸-0.5mol/L的K2SO4直接浸提，280nm紫外比色法和熏蒸-淹水培养法测定了20种有机质、全氮和速效氮差异较大的土样的土壤微生物量N。

The results demonstrated that supernatant IL-6(181±22U/ml) may be found after cultivation of unstimulated HUVEC for 24 hours.

HUVEC原代培养，HUVEC释放IL-6、IL-8观察，MTT比色法作IL-6活性测定，双抗夹心ELISA法测IL-8，t检验进行统计学处理。结果未刺激的HUVEC培养24小时上清IL-6活性为 181±22U/ml。

Methods MTT was used to study the proliferation of A375 human melanoma cell. Tyrosinase activity was measured by zymologic method. The melanin was detected by its absorption at 475nm.

方法四甲基偶氮唑蓝法测定验方对细胞增殖的影响，用酶学方法测定对酪氨酸酶活性的影响，475nm比色法测定验方对黑素含量的影响。

Methods With the model of aging mice induced by D-gal, the learning memory ability of mice was measured by using Y-maze, the contents of SOD and MDA in the cerebrums were determined by xanthine oxidation or TBA colorimetry ,the variations of above indices of mice after treatment with NGD were observed.

建立 D-半乳糖致小鼠衰老模型，以 Y型电迷宫检测其学习记忆能力，分别采用黄嘌呤氧化酶法、TBA比色法测定大脑 SOD活性和 MDA含量，观察小鼠服用神经生长液后上述指标的变化。

Methods: After LNCaP and PC3 cell lines were affected by 10, 25, 50, 75, 100 μmol/L curcumine respectively, the cell activity was assayed with MTT method at 5, 12 and 24 hours respectively; Electronic microscopy and flow cytometry were adopted to observe the morphological changes and the cell cycle of LNCaP and PC3 cell lines at 24 hours.

分别用10、25、50、75、100μmol／L浓度的姜黄素对LNCaP及PC3前列腺癌细胞株进行体外干预，5、12、24 h后四氮甲唑蓝比色法检测细胞生长活性；24 h后透射电镜观察细胞超微结构变化，流式细胞仪测定细胞凋亡及细胞周期的变化；5 h后Western印迹法检测细胞内IκBa的表达。

The proliferation of cells were detected by trypan blue exclusion, methyl thiazolyl tetrazolium assay, Hoechst33342 fluorescence staining and flow cytometry method.

利用台盼兰排斥试验、噻唑蓝比色法、Hoechst33342 荧光染色、流式细胞术测细胞周期法等实验技术分别检测了不同浓度AA 和DHA 对大鼠前体脂肪细胞活力、细胞周期及凋亡的影响。

At the endpoint, the thymus, spleen and uterus were weighed separately; the femoral dry weight, bone-calcium, phosphor, magnesium and hydroxyproline contents of femoral were measured; the histomorphological features of bone were observed and the biochemical indexes in serum (Ca, P, ALP, TRAP, E2, BGP) were determined after the mice were given SAR at the dose of 10, 30, 90 mgkg^(-1) respectively, and were compared with the model group, the normal control and the positive control. RESULTS: The osteoporosis of high turnover type was formed with feeding retinoic acid to experimental mice. Compared with the model group, the lessening of femoral dry weight, the short femoral transverse diameter, the decrease of bone mineral and bone-hydroxyproline of SAR groups were improved.

维A酸105mgkg^(-1致小鼠骨质疏松模型，SAR10、30、90mgkg^(-1灌胃给药，实验过程监测体重，2周后以比色法测定血清钙、磷含量和碱性磷酸酶、抗酒石酸酸性磷酸酶活性，竞争放射免疫法测定血清中骨钙素、雌二醇(s-E2)含量，处死动物后取脾、胸腺、子宫称重，取股骨进行骨组织形态学观察，测定并比较骨计量学和骨钙、骨磷、骨镁及骨弃脯氨酸含量。

In order to ascertain Auto-circular fermentation technology of Mulberry vinegar and find out the vinegar 's ability to eliminate DPP H ., the liquid fermentation techniques of Mulberry vinegar, produced by using Auto-circular fermentor , have been analyzed and the micro-organism on the Mulberry fruit surface has been observed with microscope, the total flavones content as well as the free radical-DPPH eliminating ability in several kinds of vinegars have been tested.

为了确定桑椹醋自回淋发酵工艺，并了解其对 DPPH 自由基的清除能力，以秦巴山区桑椹为原料，采用液态自回流发酵法生产桑椹醋，从原料菌群、酒精发酵主产物和醋酸发酵主产物的动态变化趋势等方面对其工艺进行了分析，并采用比色法对桑椹醋总黄酮含量及其 DPPH 自由基的清除率进行了测定。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。