比色法

MTT assay was used to assess the effects of deguelin on cell proliferation. FCM and RTPCR were used to analyze the changes of nup98 mRNA and protein in K562 cells after treating with deguelin.

用MTT比色法检测细胞增殖活性；流式细胞术及RTPCR法分析鱼藤素作用K562细胞后nup98蛋白及mRNA含量的变化。

Methods:Thirtytwo rats were randomly divided into four groups(normal control group,celiac seawaterimmersing trauma stress 1,2,3h groups).The amount of hexosamine and phospholipids in gastric mucosa of rats were detected by both chemochromometric and modified chemical methods.

32只SD大鼠随机分成正常对照组和腹腔浸泡伤1h、2h、3h 4组，分别取相应大鼠的胃黏膜，采用化学比色法和改良抗坏血酸法测定胃黏膜中氨基己糖和磷脂含量。

Urinary NAG activities were measured by nitrophenol colorimetric analysis technique.

用生化法测定血肌酐，用硝基苯酚比色法测定尿NAG。

objective to investigate the inhibition mechanism of nanoapatite on the human hepatocelluar carcinoma.methods stable and single-dispersed nanoapatite were synthesized with sol-gel method.nanoapatite were surveyed by zataplus and tem.their effect on the bel-7402human hepatoma cell lines was investigated by the mtt methods,and the mechanism was studied from changes in ultrastructure.results the result showed that inhibition of nanoapatite on the bel-7402human hepatocellular carcinoma cell lines was presended obviously in vitro.conclusion nanoapatite entered into cancer cytoplasm,and cancer cells result in oncosis.

目的 探讨纳米磷灰石的抑癌机制。方法利用sol-gel法制备出稳定单分散的纳米磷灰石体系，应用粒径电位仪和透射电镜对纳米磷灰石进行表征；采用mtt比色法研究纳米磷灰石对细胞系bel-7402人肝癌细胞的作用；从超微结构的改变研究其作用机制。结果实验结果表明，纳米磷灰石在体外对bel-7402人肝癌细胞具有明显的抑制作用。结论通过进入到癌细胞内，导致癌细胞胀亡。

Methods:Culture of aorta SMC in vitro,abdominal injection medication and drawing blood through ophthalmectomy,cytotoxic crystal violet staining for measurement of TNF, and dependent-cells MTT colorimetry for measurement of IL-6 were all performed.

体外主动脉SMC培养，腹腔注射给药及眼球摘除取血，细胞毒结晶紫染色法测定TNF，依赖细胞株MTT比色法测定IL-6。

Methods K-ras gene point mutation and its style at codon 12 of Patu 8988 cell were detected by using PCR-SSP and sequence analysis.

方法顺序特异引物聚合酶链反应法和基因测序检测Patu8988细胞K-ras点突变形式，根据点突变形式设计并合成硫代反义寡核苷酸（K-ras mutation ASODN）作用Patu 8988，通过噻唑蓝比色法检测细胞生长情况；流式细胞术检测K-ras蛋白表达和细胞凋亡；逆转录-聚合酶链反应检测K-ras mRNA表达水平；以Patu8988细胞建立裸鼠胰腺癌模型观察K-ras mutation ASODN在体内的抗肿瘤效果。

The hydroxyl free radical scavenging rate was determined by phenanthroline spectrophotometry, and the antilipoperoxidant was determined by colorimetry.

采用邻二氮菲分光光度法测定经自由基的清除率，比色法测定其对脂质过氧化的抑制作用。

In the rapid ampoule determination of low concentration iron in water phenanthroline is used as developing agent.

测定条件；油田水分析；中低浓度；吸光度；准确度；铁含量；快速测定法；铁水样；目视比色法；灵敏度

Methods:Soluble saccharides and rough polysaccharide were extracted by supersonic wave with 80% alcohol and 2% hydrochloric acid respectively and the outcome extracted with water and precipitated with alcohol was polyose extract.

分别用 80 %乙醇及 2 %盐酸超声波提取白首乌中的可溶性糖及粗多糖，采用蒽酮比色法分别测其可溶性糖及粗多糖的含量，并对白首乌水提醇沉法获得的多糖提取物进行含量测定。

Methods The two different extracting methods: Ultrasonic extraction and Soxhlet's extracter were used.

分别采用超声提取法和索氏提取法，用比色法比较维药芹菜籽中总黄酮的含量。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。