比色法

Methods:Rat hepatocytes were isolatcd by a single two step perfusion method,placed in 96 hole tissue plates and incubated for 1.5 hours,then exposed to different concentrations of HGF for 48 hours and the proliferation of rat hepatocytes was determined by MTT colorimetric assay.

利用胶原酶原位二步灌注法分离大鼠肝细胞，接种并预培养 1 。5 h后，用含不同浓度 HGF的培养基继续培养至 48h，MTT比色法检测肝细胞生长因子对原代培养大鼠肝细胞增殖的影响。

Methods The means of systematic solvent extraction was used to separate the 95% ethanol extract from squilla oratoria.

方法采用系统溶剂法对口虾蛄95%乙醇提取物进行分离，运用MTT比色法对分离得到的部位进行体外抗肿瘤活性的测定。

Methods Using methyl thiazolyl tetrazolium assay and bromodeoxyuridine ELISA,to investigate the effect of drugs on proliferation of VSMC in vitro.

采用MTT比色法和Brdu—ELISA法观察药物对离体大鼠VSMC增殖的影响。

Methods The antimicrobial peptide of M. domestica pupa were produced largely by infection and injury, which were isolated and purified by trituration, centrifugalization and column chromatography of AKTA purifier. Then the antimicrobial peptides with anti-Toxoplasma activity were selected by haemacytometry and MTT colorimetric method.

通过损伤加感染的方法诱导家蝇蛹大量表达抗菌肽，然后经过研磨、离心和AKTA purifier层析等步骤，将家蝇蛹抗菌肽进行分离纯化，采用血细胞计数法和MTT比色法筛选对弓形虫速殖子有抑制作用的抗菌肽。

objective to isolate and purify the antimicrobial peptides with anti-toxoplasma gondii activity isolated from the haemlymph of musca domestica larvae.methods the antimicrobial peptides of musca domestica larvae were induced by infection and injury were isolated and purified by trituration,centrifugalization and column chromatography.then the antimicrobial peptides with anti-toxoplasma activity were sieved by mtt colorimetric method and haemacytometry.results it was found that two antimicrobial peptides had anti-toxoplasma activity through resource s cationic column chromatography and superdex g75 gel column chromatography.conclusion the two antimicrobial peptides with anti-toxoplasma activity existing in the haemlymph of musca domestica larvae had different anti-toxoplasma effect.

作者单位：山西医科大学寄生虫教研室，太原 030001；化学生物学与分子工程教育部重点实验室山西大学生物技术研究所目的从家蝇幼虫血淋巴中分离纯化具有抗弓形虫作用的抗菌肽。方法通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽，然后经过研磨、离心和层析等过程，将家蝇幼虫抗菌肽进行分离纯化，采用四甲基偶氮噻唑蓝比色法和血细胞计数法筛选对弓形虫速殖子有抑制作用的抗菌肽。结果经resource s阳离子柱和superdex g75凝胶柱层析后，筛选出2种对弓形虫速殖子有杀伤作用的抗菌肽。结论家蝇幼虫血淋巴中存在抗弓形虫作用的抗菌肽，而且不止一种，但其效果有所不同。

DQ group included 30 cases treated by routine treatment and 30 cases treated by integrated TCM and western medicine.30 cases healthy people were enrolled as normal control group. All subjects were hemospasiaed venomously under fasting condition. ELISA measured the markers including TNF-α and IL-6 before and after treatment, as well as SOD and MDA were measured by biochemistry.

空腹抽取肘静脉血，采用ELISA法检测血清TNF-α、IL-6水平，化学比色法检测血清SOD、MDA水平，研究慢性乙型肝炎治疗前后TNF-α、IL-6、SOD、MDA及证候积分变化，探讨各指标与湿热蕴脾证、脾胃气虚证的关系。

39 Qinghai Alectoris graeca were used to study isozyme pattern of serum amylase and activity with vertical polypropylene gel electrophoresis and spectrophotometry, respectively.

方法］采用聚丙烯酰胺凝胶垂直平板电泳法对39只青海石鸡血清淀粉酶同工酶酶谱进行研究，并利用比色法时其淀粉酶活性进行测定。

RESULTS: Treated for a same period （3 d）, the inhibitory effect of MG132 on PC12 ceil proliferation enhanced with the increment of the concentration of MG132（0, 1, 2.5, 5, 10, 20 μmot/L）.

用不同浓度的MG132处理PC12细胞3d，通过3-（4，5-二甲基噻唑-2）-2，5-二苯基四氮唑溴盐微量比色法检测MG132对PC12细胞增殖的影响，通过姬姆萨染色法和流式细胞术检测MG132对PC12细胞凋亡的影响。

MethodsThe cells of human gastric cancer cell line SGC7901 was treated respectively with Chrysin at different concentration 10、20、40、80μM for 48h. The proliferation inhibitory rate was measured by MTT assay. Acridine orangestaining and flow cytometry were used to detect apoptosis. Western blot assay was used to detect the expression of apoptosis related genes NF拨B,Bcl2,Bax.

方法分别用10、20、40、80μM的ChR处理人胃癌细胞株SGC7901 48h，采用MTT比色法检测ChR对SGC7901细胞的增殖抑制效应；采用丫啶橙染色、流式细胞术检测ChR诱导SGC7901细胞凋亡的发生；应用Westernblot法检测凋亡相关基因NF拨B、Bcl2、Bax的蛋白表达，并用计算机图像分析软件进行半定量分析。

The inhibitory effects of ATRA and its nickel complexes on K562 cell lines have been investigated by using MTT colorimetric assay and clonic assay.

采用MTT比色法，克隆形成法研究了该配合物及全反式维甲酸对K562细胞增殖的影响。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。