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Urinary microalbumin was determined with immunoturbidimetry, urinary NacetylD-glucosaminidase,βDgalactosidase(GAL and glycyl proline dipeptidyl aminopeptidase were determined with rate, urinary creatinine was determined with Jaffe rate.

对127例糖尿病患者,采用免疫透射比浊法检测尿mA,速率法检测N乙酰βD氨基葡萄糖苷酶、βD半乳糖苷酶、甘氨酰脯氨酸二肽氨基肽酶,Jaffe速率法测尿肌酐。

They were separated by using DEAE-cellulose column chromatography. Their structures were determined by IR and GC-MS. The contents of the total polysaccharide, 3,6-anhydrogalactose and sulfate were analyzed by the methods of anthranone-sulfuric acid, resorcin and BaCl2 turbidimetry.

分别用蒽酮—硫酸法、间苯二酚法、BaCl_2比浊法测定多糖样品的总糖含量、3,6-内醚半乳糖含量和总硫酸基含量;用红外光谱法确定多糖样品的特征基团;用气质联用法初步测定麒麟菜硫酸多糖的化学组成。

It was studied that Apolipoproteins AⅠand B were determined by scattering immunoturbidimetric method using fluorescence spectrophotometer in stead of laser turbidimeter,and meanwhile the reference values of apolipoproteins AⅠand B of health persons were investigated.

研究了荧光分光光度计的免疫散射比浊法代替激光散射浊度仪来测定载脂蛋白AⅠ和载脂蛋白B,同时测定正常人的ApoAⅠ和ApoB的参考值。

Methods:Radioimmunoassay method was used to detect the levels of IL-6 in the cerebral spinal fluid.nephelometry method was used to detect the levels of albumin in the CSF and bromocresol green method was detect the levels of albumin in the serum.

用放射免疫法检测IL-6、溴甲酚绿法检测血清白蛋白、免疫比浊法检测脑脊液白蛋白。

The method uses first phase law to determine 63 DVT patient contrasts normally with 30 the F Ⅻ C of the group, use law of thing of the heart that deliver quality to detect at the same time zymogen of serous fine dissolve is active , law of enzymatic couplet immunity measures sex of antigen of content of activation of zymogen of methodize fine dissolve (depressor of content of activation of zymogen of dissolve of T-PAAg), fine - 1 antigen sex (PAI-1Ag) and immunity compare chaotic law to determine serous D-2 gets together body content.

方法采用一期法测定63例DVT患者和30例正常对照组的FⅫC,同时采用发色底物法检测血浆纤溶酶原活性,酶联免疫法测定组织型纤溶酶原激活物抗原性、纤溶酶原激活物抑制剂-1抗原性(PAI-1Ag)及免疫比浊法测定血浆D-二聚体含量。

After 24 hours, all rats were anesthetized again and their left common carotid arteries were cannulated to facilitate blood withdrawal for serum sample. The contents of tumor necrosis factor-a and interleukin-6 (IL-6) in serum were determined before and after experiment by ELISA; the activity of superoxide dismutase and content of malondialdehyde were determined by xanthinoxidanse method and modified TBA microdetermination. The content of NO2(superscript -)/NO3(superscript -), and activity of nitric oxide synthase and inducible NOS were determined by nitrate reductase method, and chemical chromogenic reaction, respectively; the immunoglobulin G, IgA, IgM and complement 3 (C3) and C4 were determined by immunoprecipitation method before and after experiment.

实验评估:手术创伤后24h进行采用酶联免疫吸附法测定实验前后血清肿瘤坏死因子α、白细胞介素6含量;应用黄嘌呤氧化酶法测定超氧化物歧化酶活性、改良TBA微量法测定丙二醛水平;以硝酸还原酶法、化学显色法分别检测NO2/NO3浓度及一氧化氮合酶、诱导型一氧化氮合酶活胜;以免疫比浊法经全自动生化分析仪检测血清免疫球蛋白IgG、IgM、IgA及补体C3、C4含量。

Nephelometric method is considered to be the best method for clinical determination of RF,which is shown to be high specificity and better reproducibility.

比浊法明显优于乳胶法,速率散射比浊法敏感性高,重复性好,是临床检测RF的优选方法。

The diagnostic sensitivity and specificity of turbidimetric assay were 88.60% and 95% respectively.

透射比浊法和散射比浊法检测RF的结果有显著的相关性,具有相似的临床诊断价值。

Methods Transmission turbidity and turbidimetric assay were used respectively to detect serum rheumatoid factors from 35 RA cases and 60 healthy controls and the results were analyzed by statistics.

用透射比浊法和散射比浊法检测35例RA患者和60例健康体检者血清RF值,经统计学处理进行分析比较。

Methods RF was measured by the rate scatting immunity method.

方法对 RA患者 12 7例、非 RA其他风湿病患者 10 2例及正常对照组 4 3例,采用速率散射免疫比浊法检测 RF;酶联免疫吸附法定量检测抗- CCP抗体;免疫荧光法检测 AKA、APF,并采用四格表法计算敏感度及特异性。

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