比及

The efficiency of selection was monitored by comparing the number of phage recovered from the acid elution and cell lysate in each round,and by testing EGFR binding specificity of polyclonal phagescFv on CHOEGFRGFP1 and CHOK1 cell with cell ELISA. Bacterial PCR was used to select clones containing a 1 kb insert. Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

Firstly, for the oxidation reactions of 1,2,4-trimethylbenzene to trimellitic acid, a simplified first-order series-parallel model was proposed, and kinetic equations in constant catalyst concentration and fixed solvent ratio were set up according to the above model.

首先，提出了偏三甲苯液相空气氧化为串、并联一级反应的简化模型。在固定催化剂浓度及溶剂比条件下，建立了偏三甲苯氧化及生成偏苯三酸的动力学方程。

Beyonce knowles唱的啊 You need to stop playing round with all them clowns and the wangstas Good girls gotta get down with the gangstas Go head girl put some back and some neck up on it While I stand up in the background and check up on it Ooh Boy you looking like you what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight If you got it, flaunt it, boy I know you want it While I turn around you watch me check up on it Oohhh you watchin me shake it, I see it in ya face 'n' Ya can't take it, it's blazin, you watch me in amazement You can look at it, as long as you don't grab it If you don't go braggin, I'm a let you have it You think that I am teasin, but I ain't got no reason I'm sure that I can please ya, but first I gotta read ya Ooh Boy you looking like you like what you see Won't you come over check up on it (I'm gonna let you work up on it) Ladies let em check up on it (watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight (x2) I can tell you wanna taste it, but I'm gone make you chase it Got to be patient, I like my men patient More patience, you take might get you in more places You can't be abrasive, has to know to pace it If I let you get up on it, you gotta make a promise That you gon put it on me, like no ones put it on me Don't bore me, just show me, all men talk but don't please I can be a tease, but I really wanna please you Ooh Boy you lookin like you like what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight (x2) I'm checking on you boo, do what chu do And while dance I'ma glance at this beautiful view I'm keep my hands in my pants, I need to glue em w/ glue I'm in a trance all eyes on you and your crew Me and my mans don't dance, but to feel ya'll bump and grind If won't hurt if you gone try one time They all hot, but let me see this ones mine Its slim thug and DC outta H town Ohh Boy you looking like you like what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight x4

slimthug，你必须停止玩回合与所有这些小丑与wangstas好女孩gottagetdown 随着gangstas走头女孩把一些背部和颈部一些比它在我站起背景及检查它oohboy你看你喜欢什么，你会看到你不来对清理出来，(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonme今晚如果你有它，炫耀它，男孩我知道你要它当我转身，你看我检查它oohhh你watchin 我摇，我看它在亚面子'n'ya不能把它，它的blazin，你看我一无所有你可以看看，只要你不抓这个问题，如果你不去braggin，我是一个让你如果你觉得我teasin，但我是不会有任何理由，我相信我能请娅但是，我首先gotta阅读娅ooh男孩找你喜欢你喜欢你见到不会你来超过清理它(我gonna让你工作起来就)让各位em检查它沾，流行资讯，twirk它，阻止它， checkonmetonight(x2)，我可以告诉你想品尝，但我已离开人世，使你追逐它必须有耐心，我喜欢我的男病人更耐心，你也许可以得到你的地方越来越多，你不能磨料已经知道它的速度，如果我让你起床，那 yougotta做出承诺，你gonputitonme，不一样的说得对我并不负有我，只告诉我，所有男人说话，但不讨人喜欢，我可以戏耍，但是我真的想请你oohboylookin你喜欢你喜欢你见到不会你来对清理出来，(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonmetonight(x2)我查你boo，做什么不楚而舞i'ma一眼这个美丽的看法，我把我的手在我的裤子，我需要胶水emw/胶水我在发呆都看你和你的船员和我我mans不跳舞，但感觉ya'll碰及研磨如果不会伤害如果你去尝试一次他们都热，但让我看到这样的矿井其slimthug直流outtah镇ohhboy你看喜欢你喜欢你见到不会你来清理，那(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonme今晚x4

Slim Thug You need to stop playing round with all them clowns and the wangstas Good girls gotta get down with the gangstas Go head girl put some back and some neck up on it While I stand up in the background and check up on it Ooh Boy you looking like you what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight If you got it, flaunt it, boy I know you want it While I turn around you watch me check up on it Oohhh you watchin me shake it, I see it in ya face 'n' Ya can't take it, it's blazin, you watch me in amazement You can look at it, as long as you don't grab it If you don't go braggin, I'm a let you have it You think that I am teasin, but I ain't got no reason I'm sure that I can please ya, but first I gotta read ya Ooh Boy you looking like you like what you see Won't you come over check up on it (I'm gonna let you work up on it) Ladies let em check up on it (watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight (x2) I can tell you wanna taste it, but I'm gone make you chase it Got to be patient, I like my men patient More patience, you take might get you in more places You can't be abrasive, has to know to pace it If I let you get up on it, you gotta make a promise That you gon put it on me, like no ones put it on me Don't bore me, just show me, all men talk but don't please I can be a tease, but I really wanna please you Ooh Boy you lookin like you like what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight (x2) I'm checking on you boo, do what chu do And while dance I'ma glance at this beautiful view I'm keep my hands in my pants, I need to glue em w/ glue I'm in a trance all eyes on you and your crew Me and my mans don't dance, but to feel ya'll bump and grind If won't hurt if you gone try one time They all hot, but let me see this ones mine Its slim thug and DC outta H town Ohh Boy you looking like you like what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight x4

slimthug，你必须停止玩回合与所有这些小丑与wangstas好女孩gottagetdown 随着gangstas走头女孩把一些背部和颈部一些比它在我站起背景及检查它oohboy你看你喜欢什么，你会看到你不来对清理出来，(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonme今晚如果你有它，炫耀它，男孩我知道你要它当我转身，你看我检查它oohhh你watchin 我摇，我看它在亚面子'n'ya不能把它，它的blazin，你看我一无所有你可以看看，只要你不抓这个问题，如果你不去braggin，我是一个让你如果你觉得我teasin，但我是不会有任何理由，我相信我能请娅但是，我首先gotta阅读娅ooh男孩找你喜欢你喜欢你见到不会你来超过清理它(我gonna让你工作起来就)让各位em检查它沾，流行资讯，twirk它，阻止它， checkonmetonight(x2)，我可以告诉你想品尝，但我已离开人世，使你追逐它必须有耐心，我喜欢我的男病人更耐心，你也许可以得到你的地方越来越多，你不能磨料已经知道它的速度，如果我让你起床，那 yougotta做出承诺，你gonputitonme，不一样的说得对我并不负有我，只告诉我，所有男人说话，但不讨人喜欢，我可以戏耍，但是我真的想请你oohboylookin你喜欢你喜欢你见到不会你来对清理出来，(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonmetonight(x2)我查你boo，做什么不楚而舞i'ma一眼这个美丽的看法，我把我的手在我的裤子，我需要胶水emw/胶水我在发呆都看你和你的船员和我我mans不跳舞，但感觉ya'll碰及研磨如果不会伤害如果你去尝试一次他们都热，但让我看到这样的矿井其slimthug直流outtah镇ohhboy你看喜欢你喜欢你见到不会你来清理，那(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonme今晚x4

Crude death rate is 201.55 per 100 thousand, and standardization is 184.04 per 100 thousand, which is 43.93% higher than the average level of the whole country. The standardization are as follows: hepatoma (53.57 per 100 thousand), lung cancer (47.76 per 100 thousand), gastric cancer (31.17 per 100 thousand), breast carcinoma (5.18 per 100 thousand), colorectal cancer (8.6 per 100 thousand). Monitoring indicates that the aggravation of water, atmosphere, small marine products, and the unhealth life habits are the main facts. Earlier screening profits diagnosis and prevention beforehand.

结果 慈溪市近十年恶性肿瘤死亡率逐年上升，位全死因的第一位，粗死亡率为201.55/10万，标化死亡率184.04/10万，比全国平均高43.93%，恶性肿瘤顺位及标化死亡率以肝癌(53.57/10万)、肺癌(47.76/10万)、胃癌(31.17/10万)、乳腺癌(5.18/10万)、结直肠癌(8.6/10万)，大气、水、土壤、蔬菜、小海产品监测表明：水质、环境的恶化及本市区域的大气、小海产品局部污染和不良卫生习惯是恶性肿瘤发生的主因，早期筛查利于早发现、早预防。

Through measuring the gel time and the unconfined compression stre- ngth of cement-waterglass-clay specimen, this paper, using orthogonal method, analyzes the effect of different water-cement ratio, different admixing quantities of waterglass and different clay content on specimen strength and presents the optimal dispensation formula for two-fluid process of cement-waterglass.

本文通过水泥—水玻璃—粘土试件凝胶时间的测定及无侧限抗压强度测定，用正交方法分析了不同水灰比、不同水玻璃掺量及不同粘土含量对试件强度的影响，提出了水泥—水玻璃双液浆的最优配方，基本解决了旋喷施工时造成的基础瞬时沉降问题，为旅喷注浆的设计与施工提供了依据。

Based on the requisition of wireless system debugging, this paper develops a novel radio-frequency beaconing. Design method and system constitution of a novel beaconing based on PLL IC PE3336 are given, and the accomplish method of the system's hardware and software arc expounded. The test results show that the beaconing adopted in this method has a reliable and stable frequency conversion features. The more applications in the wireless system find that the users can set necessary frequency conveniently. Especial1, the friendly man-machine interface makes the operation is more flexible. It is as excellent performance-price-ratio beaconing, and as a signal source, it can be used in the research and the debug procession of variable radio-frequency components.

根据无线电数据链系统及部件的调试需要，研制开发了一种新型的以锁相环为核心、由单片机控制的射频信标机；文中给出了基于锁相环芯片PE3336的新型信标机的设计方法与系统组成，阐述了系统硬件及软件的具体实现为法；测试结果表明，利用该方法设计的信标机具有稳定、可靠的变频特性，且各项指标均达到了要求；进一步在某无线电系统中的应用发现，用户可方便地设置所需频点；特别是友好的人机界面，使用户操作更为灵活方便，是一种性能价格比优良的信标机；并可作为信源用于不同的射频部件研制与调试过程中。

Experiments on synthetic images with low signal-to-noise ratio images and nature images show good performmance and robust of the approach, especially it's capable to capture the discontinuous boundary and move snakes into boundary concavities.

对人工合成的低信噪比图像及自然图像的实验表明，本文方法性能良好，工作稳定，具有较强的处理边缘不连续及进入凹形边缘能力。

MgO-C brick has a good erosion resistance but Al〓O〓-C brick is better. Corrosion mechanism for refractory without carbon is that FeO and oxides of molten slag take place chemical reaction with constituents of refractory and forming low fusing point compounds to be dissolved in molten slag. For those refractory containing graphite carbon is to be decarburized in advance and to form decarburizing layer and metamorphosis layer. The subsequent corrosion is decarburizing and taking chemical reaction to form low fusing point compounds and dissolved in molten slag at the same time.

铁浴中硅和钛的扩散是影响铁浴及熔渣与耐火材料作用的重要因素，耐火材料中添加的TiO〓和SiC受熔渣氧化和侵蚀，将被部分还原形成Si，Ti向铁浴中扩散，Si、Ti在铁浴中的扩散研究结果是： D〓=1.84×10〓exp（－5988.7/T） cm〓/S E〓=49766 J/mol D〓=7.76×10〓exp（－53225.5/T） cm〓/S E〓=442304 J/mol 铁浴式熔融还原熔体对粘土砖，高铝砖，镁砖的侵蚀速度很高，镁碳砖抗侵蚀能力比前几种耐火材料强，但不如铝碳砖；对不含碳耐火材料在熔体中的侵蚀是熔渣中FeO及其它氧化物与耐火材料组分的化学反应形成低熔点物熔蚀进入渣相，产生损毁。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。