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Analyzing the change of DH, PCL and active calcium, and selecting each of best method. The results:(1) Dealing with the bone under high pressure ①High content of protein in sheep bone or bone soup:0.05MPa, 2h, 1:2;②Low amino acids in bone powder:0.13MPa, 4h, 1:3; Low amino acids in bone soup: 0.10MPa, 3h, 1:4;(2) Hydrolysis the bone soup with enzymes ①High content of calcium: neutral enzyme, 1500u/L, 6h;②High DH: pancreatic enzyme, 1:100, 6h;③ Low PCL: Pancreatic enzyme, 1:125, 6h; High PCL: alkalescency enzyme,

结果表明:A、高压①骨粉及骨汤蛋白质含量最高:压力为0.05MPa,高压2.0h,骨水为1:2;②骨粉中氨态氮最低:压力0.13MPa,高压4.0h,骨水为1:3;骨汤中氨态氮最低:0.10MPa,3.0h,骨水1:4;B、酶解①游离钙含量最高:中性蛋白酶,酶浓度1500u/L,酶解6h;②水解度最高:胰蛋白酶,酶浓度1:100,酶解6h;③PCL值最低:胰蛋白酶,酶浓度1:125,酶解6h;PCL值最高:碱性蛋白酶,酶浓度1500u/L,酶解8h。

The results:(1) Dealing with the bone under high pressure ① High content of protein in sheep bone or bone soup:0.05MPa, 2h, 1:2;② Low amino acids in bone powder:0.13MPa, 4h, 1:3; Low amino acids in bone soup: 0.10MPa, 3h, 1:4;(2) Hydrolysis the bone soup with enzymes ① High content of calcium: neutral enzyme, 1500u/L, 6h;② High DH: pancreatic enzyme, 1:100, 6h;③ Low PCL: Pancreatic enzyme, 1:125, 6h; High PCL: alkalescency enzyme,

结果表明: A 、高压①骨粉及骨汤蛋白质含量最高:压力为0.05MPa ,高压2.0h ,骨水为1:2;②骨粉中氨态氮最低:压力0.13MPa ,高压4.0h ,骨水为1:3;骨汤中氨态氮最低:0.10MPa ,3.0h ,骨水1:4; B 、酶解①游离钙含量最高:中性蛋白酶,酶浓度1500u/L ,酶解6h ;②水解度最高:胰蛋白酶,酶浓度1:100,酶解6h ;③ PCL 值最低:胰蛋白酶,酶浓度1:125,酶解6h ; PCL 值最高:碱性蛋白酶,酶浓度1500u/L ,酶解8h 。

The linear ranges for aminopyrine,antipyrine and barbital were 1.67~333 mg/L,133~667 mg/L,60~300 mg/L respectively.

氨基林、安替林、巴妥线性范围分别为1.67~333、133~667、60~300 mg/L,检出限分别为1、4、12 mg/L。

Both the number of input nodes and hidden nodes was 5.Refrence solution was mixure of aminopyrine and antipyrine.

输入节点数和隐含节点数均为5,测定参液为氨基林和安替林的混合液。

A ratio spectra derivative spectrophotometry for simultaneous determination of quaternary mixture of aminopyrine, phenobarbital, caffeine and phenacetin was studied.

提出了可应用于四元混合体系同时测定的光谱-导数分光光度法,阐述了该方法的基本原理,并将其应用于氨基林、苯巴妥、咖啡因和非那西丁四元混合体系中各组分的同时测定。

Refrence solution was mixure of aminopyrine and antipyrine.

输入节点数和隐含节点数均为5,测定参液为氨基林和安替林的混合液。

METHODS:Aminopyrine and antipyrine in Antongding injection were determined by radial basis fuction neural network UV spectrophotometry,barbitial was determined with absorbance substraction technique.

采用径向基函数紫外分光光度法同时测定安痛定注射液中的氨基林和安替林,并结合吸光度减技术测定巴妥。

A method based on liquid phase microextraction with gas chromatography-flame ionization detector was established for the determination of aminopyrine,antipyrine and barbital in Urine.

建立了一种以液相微萃取(Liquid Phase Microextraction,LPME)与气相色谱-氢火焰(LPME-GC-FID)联用技术为基础的测定尿样中氨基林、安替林和巴妥的方法。

A newly developed multivariate calibration method——hybrid linear analysis was applied to determine aminopyrine, antipyrine and barbitone in antongding injection without separation.

将一种新近发展的多元校正方法———混合线性分析法,用于安痛定注射液中氨基林、安替林、巴妥含量的同时测定。

The transfer behavior of pyrazolone derivatives, such as antipyrine, aminopyrine, 4-aminoantipyrine and analgin, across the W/NB interface was investigated. The three medicines studied can form complex with a proton and the complex formed can transfer reversely across the W/NB interface and the transfer process was controlled by diffusion. The transfer of protonated analgin was not observed within potential window because of its high transfer potential.

研究了吡唑啉酮类的安替林、氨基林、4-氨基安替林和安乃近四种药物在W/NB界面的转移行为,前三种药物在一定pH值的水溶液中结合一个质子形成1∶1质子配合物,并能在W/NB界面发生可逆转移,其转移过程受扩散控制,安乃近的质子化物由于有较高的转移电位而未在电位窗内观察到其转移峰。

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