毒蛾

The integrative control of tea tussock moths that considerd the interaction of tea leaves-pest-na...

茸毒蛾的综合防治，充分利用茶叶———害虫———天敌相互作用的生态调控作用，发展生物制剂、农业防治、生态防治等结合的综合治理方法，尽力减少化学农药使用量或者不用化学农药，建立可持继发展的害虫综合防治体系。

Tea tussock moths ; Biology ; Integrative control

茶叶茸毒蛾；生物学特性；综合防治

Type genus of the Lymantriidae; a pest (Lymantria means 'destroyer').

毒蛾科的模式属；一种害虫（lymantria 的意思是&破坏者&）。

Insect feeding test showed that after 30 days feeding Lymantria dispar larva with potted plant tender leaves, the mortality rate of Lymantria dispar larva fed with every transgenic line was over 90%, even up to 98.9%; but the mortality rate of larva fed with non-transgenic plants was only 10%.

7饲虫实验结果表明，盆栽苗幼嫩叶片饲喂舞毒蛾幼虫30d后，各个转基因株系幼虫死亡率均在90%以上，最高达到98.9%；而非转基因植株幼虫死亡率仅为10%。

These results demonstrated that BmCPV,DsCPV and LdCPV were homologous as classfied in the same electropheres type I,and that this RT\|PCR assay could be used for early,rapid,sensitive and specific detection of DsPCV infection in the natural population of the pine moth,due to the apparent different feeding habits of Bombyx mori,Dendrolimus ssp.and Lymantria dispar larva each other and the no infection of BmCPV and LdCPV to Dendrolimus ssp.

由于松毛虫与家蚕、舞毒蛾相互之间食性不同，而且BmCPV和LdCPV对松毛虫无感染性，即在松毛虫体内不会有BmCPV病毒和LdCPV病毒的感染，因此该结果一方面从分子水平上证实了DsCPV与BmCPV、LdCPV存在基因同源性，同时也表明了依据BmCPV的基因序列设计引物对DsCPV基因组核酸建立的RT-PCR扩增体系，可以作为松毛虫种群中DsCPV的一种敏感、特异、早期、快速的检测手段。

When compared with S7 and its putative protein of Lymantria dispar cypovirus 1 and Bombyx mori cypovirus 1, 97.2% and 87.0% identities in the nucleotide level, and 98.7% and 92.8% identities in the amino acid level were found, repectively. BLAST search program revealed some similarity between DpCPV-HN P50 and DnaK-like protein of Mycoplasma hominis.

该基因组与舞毒蛾质多角体病毒1型和家蚕质多角体病毒1型S7节段有很高的同源性，核苷酸序列同源性分别为97.2%和87.0%，氨基酸序列同源性分别为98.7%和92.8%。P50多肽与人型支原体的DnaK样蛋白在C-末端有相似性。

Subclones of the hybrid 741 took on a high insect-resistant identity to the larvae of leaf pests such as Clostera anachoreta Fabricius, Lymantria dispar Linnacus, Hyphantria cunea Drury ect., and the mean death rate of the grubs were all above the 85%, furthermore, the grubs' growth which survived could be restrained and the growth rate was reduced so that they couldn't tie cocoons as the usual.

项目简介：转双抗虫基因741杨是将部分改造BtCryIAc 基因与慈菇蛋白酶抑制剂基因，通过农杆菌介导法转化了优良白杨杂种741杨，并获得了一批具有高抗虫性无性系，对舞毒蛾和杨扇舟蛾，美国白蛾等鳞翅目幼虫死亡率达85%以上，并能抑制存活幼虫的发育，使其不能正常化蛹。

A new picorna-like virus was isolated from dead Evtropis oblique larvae deceased from NPV infection. Electron microscopic observations of purified virions were non-enveloped isometric particles with diameter of 26nm. The virions contain two capsid proteins: 31.5kDa and 28.8kDa, the amount of later is 2.5 times more than that of the former. Analysis of 3′terminal sequence of EoPV clone identified that it can encode RdRp and has eight conserved motifs. Homology analysis shows that it is closely related to Perina nuda picorna-like virus.

从核型多角体病毒感染致死的茶尺蠖幼虫尸体中分离到一株微小RNA病毒透射电镜观察纯化的病毒粒子为无囊膜、无表面特征、直径约26nm的球状颗粒。16%的SDS-PAGE显示它有两个分子量为31.5 kDa和28.8 kDa的衣壳蛋白，后者的含量是前者的2.5倍。3′端克隆序列分析表明EoPV基因组3′有poly尾，编码RNA聚合酶，含有微小RNA病毒RNA聚合酶的八个保守基序，同源性分析表明它与榕透翅毒蛾微小RNA病毒亲缘关系最近。

Foreign gene could destroy larvas mid-intestine, and reduce its appetite. Observation by microscope showed that the gaps between mid-intestine cells was extended in treated larvae, and the nucleus were destroyed, the gap between peritrophic membrane and mid-intestine cells was appeared, parts of peritrophic membrane was broken off from mid-intestine cells.

外源基因对舞毒蛾幼虫的杀虫机理在于破坏幼虫的中肠组织，影响幼虫的取食，使幼虫取食量减少，甚至终止取食；中肠细胞间隙增大，细胞核遭受破坏；围食膜与中肠细胞之间出现空隙，甚至脱落，围食膜不再完整；中肠组织脱落，成为一个严重变形的残架。

The highest mortality occurred at the boring stage of young instars larvae, especially in early stage of 1st and 2nd instar larva.

经存活曲线分析得知舞毒蛾种群的个体死亡主要发生在幼龄幼虫期，特别是对1，2龄幼虫的致死能力更为明显。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。