毒基

One hand mechanical obstruct led to the increase of veinous resistance and the obstacle of microcirculation, the other hand the adhesive PMN was activated in excess, the white blood cells released a lot of enzymes, in which PMN-elastase can decompose the components of cell and many albumens, inclusive of immunoglobulin、alexin and fibrication. These components induced the injury of the pancreatic capillary vessels and cell and lysosome enzy made the tissue protein hydrolyze and produced unsaturated fatty acids, which destroyed the structure and function of cellar membrane. The inflammatory cellar factors activate other immunocytes to produce the injury and necrosis of tissue, which aggravated the pathological injury and led to shock、pyaemia and MODS. So ICAM-1 and LFA-1 played an important role in SAP. Frossard found that the expression of ICAM-1 in the rat model, especially in serum、pancreas and lung. All these showed ICAM-1 is an important factor in AP and concomitant lung injury.

胰腺小叶组织局部血管EC首先被激活，ICAM-1表达升高，与被激活的PMN表面表达的LFA-1相结合，"PMN-EC"相互作用加剧，一方面机械性阻塞毛细血管导致静脉阻力增加、微循环障碍；另一方面粘附的PMN过度吞噬或激活，当白细胞吞噬的颗粒不能被封闭隔离，连同细胞内的酶被释放出来，其中的PMN-elastase能够降解细胞基质中各种成分，水解多种蛋白，加重胰腺的毛细血管内皮细胞和腺泡的损伤；释放的溶酶体酶使组织蛋白水解，产生的不饱和脂肪酸引发脂质过氧化方应，破坏细胞膜的结构和功能；释放的炎性细胞因子，激发其他的免疫细胞的功能，导致进一步的组织损伤和坏死，加重SAP的病理损伤，最终导致休克、脓毒血症及多器官功能障碍等严重后果。

Huwentoxin-XVIIb1 (HWTX-XVIIb1) is a novel peptide neurotoxin, isolated and purified from the Chinese bird spider Selenocosmia huwena Wang. It contains 31 amino acid residues including 8 cysteines.

虎纹捕鸟蛛毒素-XVIIb1 (HWTX-XVIIb1)是从虎纹捕鸟蛛粗毒中提取、表达、分离纯化的一种新型多肽，它由31个氨基酸残基组成，含8个半胱氨酸。

Medium with higher concentration sucrose can promote bulblet enlargement but inhibit bulblet formation. The best matrix for the highest surviving rate and best growth situation was pearlite/soil (3:1). The number of bulb scales surviving and regenerating decreased significantly as the increasing of days after the treatment of high temperature 37oC. The regeneration rate can be about 40% for 20days. The effectiveness of eliminating LSV with the method got to more than 95% which was significantly higher compared with the control.

蛭石：土、珍珠岩以及珍珠岩：土作为基质时，均能达到较高的成活率和株高，但在珍珠岩：土为3：1作为基质移栽组培球，其成活率和生长状况最佳。37℃热处理随着处理天数的增加，处理后培养鳞片的成活率和子球再生率显著下降，但处理20天子球再生率只能达40%左右，但LSV检出率明显低于未处理的种球，脱毒率达95%以上。

The hepatocytes from vitamin E deficience rats were more sensitive to the toxicity of TNT, and supplement of vitamin E, treatment of desferrioxamine and dithiothreitol could prevent hepatocytes from toxicity of TNT.

明显降低TNT对肝细胞的毒性，其中尤以二硫苏糖醇的效果最为明显，这些结果从另一方面证实了，自由基反应，脂质过氧化过程和由此引起的细胞内巯基耗竭是TNT毒作用的重要环节。

In conclusion, the nitro group is the main toxic group for both nitrobenzenes and nitroanilines. Wrapping or reducing the nitro groups will decrease the toxicity of the subject chemicals.

总之， 硝基是这两类化合物致毒的主要基团，将硝基包覆或还原为氨基应为此类化合物解毒的重要途径。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

During the process of sepsis, trace dose of leptin injected peritoneally significantly improves and stabilizes the hepatic and renal functions. The mechanisms may be related with those intestinal enzymes associated with metabolism of free radicals, mercapto group and purine. Indomethacin exerts a similar role as leptin though at much higher dose.

在脓毒症过程中，微量leptin体外注射可以发挥改善和稳定肝肾功能的明显作用，其机制可能同其影响肠道多种与自由基、巯基和嘌呤代谢相关酶的功能有关，而且吲哚美辛在一定程度上有类似的效应，但所用剂量明显高于leptin水平。

Researchers from the universities of Pavia and Milan analyzed several hair samples that had been taken during different periods of Napoleon Bonaparte's life -- from when he was a boy in Corsica, during his exile on the Island of Elba, on the day of his death on the Island of Saint Helena, and on the day after his death.

多年来，学者们对于拿破仑为什么会在1821年5月5日英年早逝争论不休。他的私人医生佛朗西斯科。安东马尔基验尸后得出的结论是拿破仑死于胃癌。但安东马尔基的报告的真实性在1961年遭到质疑。最近一次在2001年在拿破仑的头发中发现了高含量的砷，各种关于投毒的阴谋论接踵而至。根据那些说法，为了防止这位前法兰西皇帝逃脱流放并重返帝位，他被毒死了。

Free radical hurts biological large molecules, lets lipid peroxid- ize and damages membrane, the weakening of antiperoxidation system in vivo (vit E, SOD, GSH-PX) will increase the harmful effect of radical on cells.

自由基损伤生物大分子，使脂类过氧化，造成膜损伤；机体抗氧化体系(维生素E，超氧化物歧化酶，谷胱甘肽过氧化物酶等)作用的削弱会加重自由基的细胞毒作用。

Porfiry Petrovich, who first appeared in Feodor Dostoevsky's "Crime and Punishment", and his assistant Pavel Virginsky are dispatched to find out why a doctor's wife and handicapped son are murdered by poisoned chocolates, and a womanising army officer is shot dead in his rooms while his French paramour slumbers peacefully in his bed.

卜菲力·彼得罗维奇最早出现在陀思妥耶夫斯基的《罪与罚》中，他和助手佩福尔。维京斯基受命调查一宗谋杀案。在这个案件中，有人在巧克力里下毒，毒死了一名医生的妻子和他身患残疾的儿子；还有一个军官贪恋美色，在自己的房间里遭枪杀，而与他同床的法国小情人却安然无恙，且毫无察觉。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。