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The compounds 1～6:β-Amanitin、α-Amanitin、phallisacin、phallisin、phallacidin andphalloidin.As the content of compound7, compound 8 and compound 9 were all to little.

化合物1～6分别为：β-鹅膏毒肽、α-鹅膏毒肽、羧基三羟鬼笔毒肽、三羟鬼笔毒肽、羧基二羟鬼笔毒肽和二羟鬼笔毒肽。

In all, A.subjunquillea.S.lmai methyl alcohol crude extract were research by high pressure liquidchromatography,.separation and purification obtained to 9 kind of compounds, identified 6 kind ofcompounds. The compounds 1～6:β-Amanitin、α-Amanitin、phallisacin、phallisin、phallacidin and phalloidin.

总之，黄盖鹅膏甲醇粗提物应用高效液相分离纯化9种单体化合物，鉴定6种分别为β-鹅膏毒肽、α-鹅膏毒肽、羧基三羟鬼笔毒肽、三羟鬼笔毒肽、羧基二羟鬼笔毒肽和二羟鬼笔毒肽。

Evaluation of IEM103 in rabbit animal model.The evaluation was based on the control-challenge study (IEM101 as control). Rabbits were laparotomized, injecteded 10〓 cfu directly into proximal small bowel, and 28 days later, challenged with 0.25--2ug CT and 10〓-10〓 cfu 4 wild toxic strains with different biotypes and serotypes. Experimental group developed antitoxic and significant vibriocidal immune responses to vaccination, and could protect no less than 2ug CT and 10〓-10〓 cfu all wild strains challenge.

IEM103的免疫原性评价：经兔肠段结扎免疫〓cfu IEM103，7天后在外周血中可检测到外周淋巴细胞分泌性抗CT IgG，血清中可检测到抗CT抗体、杀弧菌抗体，P／N均≥2，且可持续至28天。28天后用0.25-2ug CT毒素，四种毒株滨-43，吴江-2，395，1119〓cfu菌量攻毒，实验组可保护至少2ug CT攻毒，而对照组可保护1ug以下CT攻毒，两组均可保护〓cfu四种毒株攻毒（以肠积液量≥1.0ml／cm为不保护判断标准）。

According to the LC-P line of each of insecticide in the mixture and the formula of co-toxicity factor, several expected mortality and the region of additive action (expected mortality±20% expected mortality) were calculated, and according to observed mortality of mixture, the 95% confidence interval was calculated, LC-P line of expected mortality with the region of additive action and LC-P line of observed mortality with the 95% confidence interval were drawn, it was found that there was overlap between the region of additive action and 95% confidence interval and two lines crossed each other when co-toxicity coefficient was more than 100 and the co-toxicity factor was less than 20, which meant there was no significant differences between expected mortality and observed mortality, and that there was on or a few overlap between the region of additive action and 95% confidence interval when co-toxicity coefficient was more than 100 and the co-toxicity factor was more than 20 or co-toxicity coefficient was less than 100 and the co-toxicity factor was less than 20, which meant there was significant differences between expected mortality and observed mortality.

根据单剂的LC-P线和共毒因子公式，求出混剂的期望死亡率和"相加作用区间"（期望死亡率±20%期望死亡率），根据混剂的实测死亡率求出95%置信区间，画出期望LC-P线及"相加作用区间"和实测LC-P线及"95%置信区间"，发现当共毒系数大于100、共毒因子小于20时，实测LC-P线和期望LC-P线彼此交缠，期望LC-P线的"相加作用区间"和实测LC-P线的"95%置信区间"能高度重叠，表明两条LC-P线之间没有毒力差异；当共毒系数大于100、共毒因子大于20，或者共毒系数小于100、共毒因子小于-20时，实测LC-P线的"95%置信区间"和期望LC-P线的"相加作用区间"只有少量重叠或完全不重叠，体现出了实测LC-P线和期望LC-P线对供试害虫的毒力差异。

The intraperitonealLD_(50) values in mice of VES and VDPG were 0.16mg/kg and 0.39mg/kg,respectively. The intraperitoneal LD_(50) value in cockroach of VDPG andVES were 2.32μg/g and 1.87μg/g, respectively. After intraperitonealinjection of the venom in mice, the main poisoning symptoms includedlethargy, ataxia, diaphoresis, convulsion, anorexia, shortness of breath,and difficulty to open eyes, etc. Whereas the mice injected withphysiological saline acted normally.

两种粗毒都对小鼠和蜚蠊有较强的毒性，其中毒囊粗毒和电刺激粗毒对小鼠的LD_(50)值分别为0.39mg／kg和0.16mg／kg；毒囊粗毒和电刺激粗毒对蜚蠊的LD_(50)值分别为2.32μg／g和1.87μg／g体重；小鼠经腹腔注射粗毒后，出现无生气、共济失调、排汗、痉挛、食欲减退、呼吸短促、睁眼困难等症状，而注射生理盐水的小鼠活动正常。

The number of chickens in each group is 16 for SPF chicken At 4 weeks of age, chickens were eye-drop immunized with the recommended dose of commercial IBV vaccine, or vaccinated intradermally via wing with 5×106 PFU rFPV-IBVS1 or 5×106 PFU S-FPV-017. Four weeks after vaccination, chickens in all groups were challenged by bronchitis injection with a lethal dose (0.2ml 8.5EID50/0.1ml) of IBV XJ3 strain After challenge, chickens immunized with rFPV-IBVS1 and commercial IBV vaccine were all protected from death; the result indicated that rFPV-IBVS1 can protect chickens from the infection of IBV.

攻毒后的结果显示所有含rFPV-IBVS1免疫组的SPF鸡均100%保护而免于死亡；IBV弱毒疫苗免疫的SPF鸡也获100%保护，免疫以后的抗体跟踪检测证明rFPV-IBVS1诱导的抗IBV抗体水平低于IBV弱毒疫苗免疫组；攻毒后进行的IBV分离和PCR检测结果表明IBV弱毒疫苗免疫的SPF鸡能够更好地阻止攻击强毒的感染。

F$ p '毒骡 drug muleMore Chinese women duped to bedrug mules--Foreign drug traffickers are duping an increasing number of Chinese women, especially young victims, into carrying drugs into China, the General Administration of Customs (GAC has said.

更多的中国妇女受骗成&毒骡&——海关总署表示，越来越多的中国妇女，特别是年轻女子，受外国毒贩的诱骗后将毒品带进中国。

To evaluate the efficacy of the recombinant fowlpox virus vaccine against the heterotype IBV, the chickens were challenged with the homotype IBV LX4 strain and the heterotype IBV LTJ95I strain after three weeks post vaccination. Antibodies against IBV were detected in vaccinated chickens one week post inoculation. The CD4+ T-lymphocytes in the peripheral blood increased rapidly in all groups challenged with IBV, except for the vaccinated group challenged by heterotype strain and the low level of CD4+ T-lymphocytes remained until end of the experiment. In all the groups, a high level of CD8+ T-lymphocytes only was observed in the vaccinated group after challenging with IBVhomotype strain. The morbidity and the mortality of this group were 21.43 % and 0 %, respectively, which showed significant difference with other groups. In addition, the lesions of chickens and virus shedding were less in the vaccinated group challenged by IBV homotype strain comparing with other groups, but there was no difference for the average body weight of chickens in all groups.

结果显示，重组疫苗接种1周后，免疫鸡产生抗IBV的抗体；而且外周血中CD4+和CD8+ T淋巴细胞的含量略高于非免疫对照组；攻毒后，异源强毒株攻毒的免疫组CD4+ T淋巴细胞呈下降趋势，并且该组低水平CD4+的状态一直持续到试验结束，而其他组CD4+ T淋巴细胞均迅速上升，峰值达到14.5 %；同源强毒株攻毒的免疫组CD8+ T淋巴细胞呈高水平的表达，而其他组攻毒后均无明显变化；保护率结果显示，同源强毒株攻毒免疫组的发病率和死亡率为21.43 %和0 %，与其他各组相比均有显著差异；另外同源强毒株攻毒的免疫组病理损伤与异源强毒株攻毒的试验组相比明显减轻，其排毒时间和排毒量也均有所减少；强毒攻毒后所有试验组体重无显著差异。

To elucidate the molecular basis of the attenuation ofDLA-EIAV in virulence thus provide theory foundation for designing HIV vaccine, thewhole genomes of DLA-EIAV and EJAV L provirus were cloned and sequenced. Aninfectious molecular clone derived from DLA-ELAV was constructed and characterized.Three DLA-EIAV/ L chimeric viruses were constructed. Promoting efficacy of the longterminal repeat of the DLA-EIAV was charactenzed.

为了揭示我国马传贫弱毒疫苗毒力致弱及免疫保护的分子机制，为人免疫缺陷病毒及其它慢病毒的免疫预防提供借鉴，我们对马传贫驴白细胞弱毒及其亲本马强毒EIAV L株前病毒进行了全基因克隆和序列测定，比较分析了二者的前病毒基因组核苷酸序列，在此基础上构建了EIAV驴白细胞弱毒株的感染性分子克隆和三株含有EIAV强/弱毒嵌合病毒基因组的重组质粒，并对EIAV弱毒株长末端重复序列的启动子活性进行了初步研究。

To elucidate the molecular basis of the attenuation ofDLA-EIAV in virulence thus provide theory foundation for designing HIV vaccine,thewhole genomes of DLA-EIAV and EIAV L provirus were cloned and sequenced.Aninfectious molecular clone derived from DLA-EIAV was constructed and characterized.Three DLA-EIAV/L chimeric viruses were constructed.Promoting efficacy of the longterminal repeatof the DLA-EIAV was characterized.

为了揭示我国马传贫弱毒疫苗毒力致弱及免疫保护的分子机制，为人免疫缺陷病毒及其它慢病毒的免疫预防提供借鉴，我们对马传贫驴白细胞弱毒及其亲本马强毒EIAV L株前病毒进行了全基因克隆和序列测定，比较分析了二者的前病毒基因组核苷酸序列，在此基础上构建了EIAV驴白细胞弱毒株的感染性分子克隆和三株含有EIAV强/弱毒嵌合病毒基因组的重组质粒，并对EIAV弱毒株长末端重复序列的启动子活性进行了初步研究。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。