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Therapontigonus Miles could walk arm in arm with Vadeboncoeur the grenadier, Damasippus the second-hand dealer would be happy among bric-a-brac merchants, Vincennes could grasp Socrates in its fist as just as Agora could imprison Diderot, Grimod de la Reyniere discovered larded roast beef, as Curtillus invented roast hedgehog, we see the trapeze which figures in Plautus reappear under the vault of the Arc of l'Etoile, the sword-eater of Poecilus encountered by Apuleius is a sword-swallower on the PontNeuf, the nephew of Rameau and Curculio the parasite make a pair, Ergasilus could get himself presented to Cambaceres by d'Aigrefeuille; the four dandies of Rome: Alcesimarchus, Phoedromus, Diabolus, and Argyrippus, descend from Courtille in Labatut's posting-chaise; Aulus Gellius would halt no longer in front of Congrio than would Charles Nodier in front of Punchinello; Marto is not a tigress, but Pardalisca was not a dragon; Pantolabus the wag jeers in the Cafe Anglais at Nomentanus the fast liver, Hermogenus is a tenor in the Champs-Elysees, and round him, Thracius the beggar, clad like Bobeche, takes up a collection; the bore who stops you by the button of your coat in the Tuileries makes you repeat after a lapse of two thousand years Thesprion's apostrophe: Quis properantem me prehendit pallio?

米勒会挽着侍卫华德朋克尔的胳膊,达马西普会在旧货店里流连忘返,万森刺杀苏格拉底正如阿戈拉囚禁狄德罗,格利木·德·拉雷尼埃尔会做油脂牛排正如古尔第吕斯发明烤刺猬。我们见到普劳图斯著作中的高架秋千重现在明星门的气球下面,阿普列乌斯在普西勒遇见的吞剑人便是新桥上的吞刀人,拉穆的侄儿和寄生虫古尔古里翁是一对,埃尔加齐尔请爱格尔弗依把他介绍给康巴色勒斯,罗马的四个纨袴子弟阿尔色西马尔古斯、费德洛木斯、狄阿波吕斯和阿尔吉里帕乘着拉巴突的邮车从拉古尔第①出发,奥吕·热尔在孔格利奥面前没有比查理·诺缔埃在波里希内儿面前待得更长久,马尔东不是母老虎,但是巴尔达里斯卡也绝不是一条龙,滑稽人潘多拉布斯在英格兰咖啡馆里嘲弄享乐人诺曼达纽斯,埃尔摩仁是爱丽舍广场的男高音,并且在他周围有无赖特拉西乌斯扮成波白什②向人募捐,在杜伊勒里广场上掐住你的衣扣、不让你走的那个讨厌人让你在两千年以后还重复着忒斯卜利翁的那句话:"在我有急事时谁突然抓住了我的衣襟?"

Antigene peptide nucleic acid specifically inhibits MYCN expression in human neuroblastoma cells leading to cell growth inhibition and apoptosis Roberto Tonelli,Stefania Purgato,Consuelo Camerin,Raffaele Fronza,Fabrizio Bologna,Simone Alboresi,Monica Franzoni,Roberto Corradini,Stefano Sforza,Andrea Faccini,Jason M. Shohet,Rosangela Marchelli,and Andrea Pession Department of Pediatrics, University of Bologna,Sant'Orsola-Malpighi Hospital, Bologna, Italy; Department of Organic and Industrial Chemistry, University of Parma, Parma,Italy; and 3Center for Cell and Gene Therapy, Texas Children's Cancer Center, Baylor College of Medicine, Houston, Texas Abstract We developed an antigene peptide nucleic acid for selective inhibition of MYCN transcription in neuroblastoma cells, targeted against a unique sequence in the antisense DNA strand of exon 2 of MYCN and linked at its NH2 terminus to a nuclear localization signal peptide.

抗原肽核酸抑制MYCN具体表达的神经母细胞瘤细胞,导致细胞生长的抑制和凋亡罗伯托利,斯特凡Purgato ,苏埃洛卡默琳,拉菲尔Fronza ,梅奥尼博洛尼亚,西蒙娜Alboresi ,莫妮卡弗兰索尼,罗伯托Corradini ,斯特凡诺斯福尔扎,安德烈Faccini ,杰森先生Shohet , Rosangela Marchelli ,和安德烈Pession儿科,博洛尼亚大学,圣' Orsola -马尔皮基医院,博洛尼亚,意大利;有机部和工业大学化学帕尔马,帕尔马,意大利和3Center的细胞和基因治疗,德州儿童癌症中心, Baylor医学院,得克萨斯州休斯敦摘要我们开发了一种抗原肽核酸的选择性抑制神经母细胞瘤MYCN转录细胞,针对一个独特的序列反义DNA链的第2外显子,并与在MYCN 其氨基端为核定位信号肽。

The formation of martensite is accomplished by the immigration of well-defined glissile interface (121)fcc type and its misfit dislocations can produce the lattice invariant deformation on the basis of phenomenal theory of martensitic crystallography, however, LID is retarded slightly after the migration of interphase (121)fcc, i.e.a thin plate-like zone exists without LID in martensite near the well-defined interface. When the temperature reduces to the Ms point, the lattice parameter of austenite matrix is √3/2 times that of the martensite without LID. This critical condition for spontaneous transformation agrees with that the stack fault energy in matrix is less than zero according to Olson and Cohen's nucleation model.

通过模型分析表明:fcc/bcc马氏体形核与长大过程是通过(121)fcc型择优界面推移进行的,界面上的错配位错可以完成马氏体晶体学唯象理论要求的点阵不变变形,但LID要稍滞后于界面迁移,即在马氏体形核与长大过程中推移界面新相一侧存在一未发生LID的新相薄区;当相变温度达到马氏体相变点Ms时,母相奥氏体与这一薄区的晶格常数比为√3/2,这一几何条件和Olson-Cohen形核模型中要求扩展位错层错区界面能γ≤0是等价的。

In Chapter 2,I consider a queuing system in which there are two exponential servers, each having his own queue, and arriving customers will join the two queues separately, and analyze the two-dimensional Markov process representing the numbers of jobs in the two stations. A functional equation for the generating function of the stationary distribution of this two-dimensional process is derived and solved through the theory of Riemann-Hilbert boundary value problems. In Chapter 3, I consider the system with two parallel queues, in which arrivals join the shorter queue.I describe the problem of coupling and show that the generating function Fcan be continued as a meromorphic function to the whole complex plane.

全文包括三大部分:第一章介绍了基本的背景、研究进展和文章主要采用的方法;第二章研究的是有两个服务员共享的简单的排队方式的模型,即每个服务员有各自的队列,两队队长互不相关,分析了代表两个队长的二维马尔柯夫过程,推导出了二维过程的平稳分布的母函数的方程,并运用Riemann—Hilbert边值问题理论解出了母函数的表达式;第三章研究的是JSQ-PS模型(the joining the shortest queue model with processor sharing),模型是组合了加入最短队模型和处理器共享模型而成的有用排队模型。

Pearl oyster, Pinctada martensii Dunker was one of the most important cultured species to produce sea pearls in China.

马氏珠母贝又称合浦珠母贝,是世界上海水珍珠培育的主要种类,在我国南方海区广泛分布。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达,且在未成熟卵母细胞中,MⅠ期的表达明显强于GV期的表达;②RT-PCR产物克隆测序结果显示:昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp~1153bp)]完全一致;Blastn比对结果发现:在不同物种中差异较小,选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析,提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%,100%,100%,36%,100%,100%,67%,100%,100%,92%,99%,99%,99%;Clustal 1.81和Treeview构建出的分子进化树表明:在上述14种生物中昆明小鼠与中国仓鼠进化关系最近,与兔子、猪聚成一簇,与食蟹猴进化关系最远;③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段,重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

Therapontigonus Miles could walk arm in arm with Vadeboncoeur the grenadier, Damasippus the second-hand dealer would be happy among bric-a-brac merchants, Vincennes could grasp Socrates in its fist as just as Agora could imprison Diderot, Grimod de la Reyniere discovered larded roast beef, as Curtillus invented roast hedgehog, we see the trapeze which figures in Plautus reappear under the vault of the Arc of l'Etoile, the sword-eater of Poecilus encountered by Apuleius is a sword-swallower on the PontNeuf, the nephew of Rameau and Curculio the parasite make a pair, Ergasilus could get himself presented to Cambaceres by d'Aigrefeuille; the four dandies of Rome: Alcesimarchus, Phoedromus, Diabolus, and Argyrippus, descend from Courtille in Labatut's posting-chaise; Aulus Gellius would halt no longer in front of Congrio than would Charles Nodier in front of Punchinello; Marto is not a tigress, but Pardalisca was not a dragon; Pantolabus the wag jeers in the Cafe Anglais at Nomentanus the fast liver, Hermogenus is a tenor in the Champs-Elysees, and round him, Thracius the beggar, clad like Bobeche, takes up a collection; the bore who stops you by the button of your coat in the Tuileries makes you repeat after a lapse of two thousand years Thesprion's apostrophe: Quis properantem me prehendit pallio?

米勒会挽着侍卫华德朋克尔的胳膊,达马西普会在旧货店里流连忘返,万森刺杀苏格拉底正如阿戈拉囚禁狄德罗,格利木·德·拉雷尼埃尔会做油脂牛排正如古尔第吕斯发明烤刺猬。我们见到普劳图斯著作中的高架秋千重现在明星门的气球下面,阿普列乌斯在普西勒遇见的吞剑人便是新桥上的吞刀人,拉穆的侄儿和寄生虫古尔古里翁是一对,埃尔加齐尔请爱格尔弗依把他介绍给康巴色勒斯,罗马的四个纨袴子弟阿尔色西马尔古斯、费德洛木斯、狄阿波吕斯和阿尔吉里帕乘着拉巴突的邮车从拉古尔第①出发,奥吕·热尔在孔格利奥面前没有比查理·诺缔埃在波里希内儿面前待得更长久,马尔东不是母老虎,但是巴尔达里斯卡也绝不是一条龙,滑稽人潘多拉布斯在英格兰咖啡馆里嘲弄享乐人诺曼达纽斯,埃尔摩仁是爱丽舍广场的男高音,并且在他周围有无赖特拉西乌斯扮成波白什②向人募捐,在杜伊勒里广场上掐住你的衣扣、不让你走的那个讨厌人让你在两千年以后还重复着忒斯卜利翁的那句话:&在我有急事时谁突然抓住了我的衣襟?&

The results show:there are a lot of dislocation martensites,a little of twincrystal martensites,remnant austenites and Cr 23 C 6 compounds.The unsufficient diffusion is the reason of the chemical composition distribution of the fusion zone.The amount of martensites and the formation of microstructure are mainly decided by the chemical composition of the base me...

结果表明,马氏体带含大量位错形马氏体、少量的孪晶形马氏体、残余奥氏体以及Cr23C6 化合物;熔合区化学成分分布特征缘于不充分的扩散传质;马氏体的数量、亚结构特征以及Cr23 C6 化合物的形成取决于母材和填充金属的化学成分。

It is considered that when temperature begins to fall down under Ms, martensite and the untransformed mother-phase are in a kind of thermobalance. We try to use the equations in marten- site transform to get the mathematical relationship between the dynamics of the microclamp and the temperature, also including some useful results for design and fabrication of the microclamp. We hope these results will form the work principle base for SMA microclamp.

认为在Ms点以下,马氏体与未转变的母相奥氏体处於某种平衡状态,利用变温马氏体相变公式,处理SMA微夹钳的热力学和动力学之间的定量或定性的数学关系,得出一些对於设计和制造有用的结论,从而为深入研究SMA微夹钳的热弹性马氏体相变的工作原理打下理论基础。

Histologically, the seminiferous tubules contained numerous Sertoli cells and more Sertoli-spermatozoa complexes, accompanied by the depletion of Leydig cells with deeply stained nuclei. Mature spermatozoa were stored up in the epididymis, but only a few in the efferent ducts. In the second place was testicular atrophy(32/120; 26.7%). The seminiferous tubules showed moderate to severe inactivity of spermiogenesis with evidence of only spermatogonia, spermatocytes and Sertoli cells. The Leydig cells were obviously decreased in numbers associated with decrease of lipid droplets in their cytoplasms. Testicular hypoplasia was the third disorders(22/120; 18.3%). Only a few spermatogonia and Sertoli cells appeared without any spermiogenesis. The associated changes was decreased in Leydig cells and fibrous hyperplasia in the interstitium.Epididymal stones were sometimes found(12/120; 10%). Grossly, yellowish-white nodules with various sizes and firm in consistency were observed in the epididymis and the front efferent ducts. Microscopically, the epididymal ducts were dilated with voluminous spermatozoa storage, even showed calcification in severe cases. The deposited calcium salts were stained positively by Von Kossa and Alizarin red methods.Amyloidosis was also detected in 10 roosters(8.3%). Eosinophilic, homogeneous, amyloid-like substances were deposited mainly in the testicular interstitium and the periphery of blood vessels. These substances showed positive reaction by Congo red staining. Five roosters(4.2%)had Marek's lesions in the testis, epididymis and peripheral nerves with infiltration of pleomorphic lymphocytes. Only one case showed epithelial necrosis of seminiferous tubules accompanied by fibrous proliferation in the interstitium.

结果发现,在总共搜集的120个病例中,其中因年老所导致的产精力不佳为最多,占38例(31.7%),於镜下可见大量精虫黏附於Sertoli cell的表面,并可见Sertoli cell数量明显增多而Leydig cell明显减少,且其细胞核呈现浓染的现象,而在其副睪中仍可见到成熟精虫蓄留於管腔中,但在其输精管内却只有少量精虫存在;其次为睪丸萎缩,占32例(26.7%),镜下可见中度至重度无造精作用,其生精小管中只见到精母细胞、精原细胞及Sertoli cell存在,但Leydig cell数量明显减少且其细胞质内的脂质也明显减少;睪丸发育不全,占22例(18.3%),於生精小管内只见到精母细胞及少量Sertoli cell存在,不见造精细胞分化,於生精小管间质可见Leydig cell减少并伴随结缔组织增生;副睪结石,占12例(10%),肉眼下可在副睪及输精管前段见到黄白色大小不一的结节,触感坚硬,於镜下可见副睪管扩张并有大量成熟精虫蓄积,严重时可见钙化现象,以Von Kossa及茜素红染色均呈阳性反应;类淀粉沉著症,占10例(8.3%),镜下在睪丸间质及血管周围可见粉红均质样的物质沉积,以刚果红染色成阳性反应;马立克病,占5例(4.2%),镜下可在睪丸、副睪实质及周边神经内均可见到嗜碱性大小不一的淋巴样细胞浸润;睪丸坏死,占1例(0.8%),镜下可见生精小管上皮细胞坏死脱落及间质结缔组织增生。

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