母质的

The experiment results were as following:All cytoplast recipients derived from oocytes of rabbit, bovine and goat can support early development of cloned embryo, the fusion rate of reconstructed embryos of goat-bovine and goat-rabbit were 59.91% and 74.10%, their cleaved rate were 57.48% and 72.57%, blastocyst rate were 9.59% and18.06% respectively, and the fusion of reconstructed embryos of goat-rabbit and goat-goat were 60.01% and 74.10%, their cleaved rate were 56.68% and 72.57%, blastocyst rate were 11.76% and18.06% respectively.

试验获得了如下结果：1。以山羊耳成纤维细胞为供体，以牛、羊、兔卵母细胞做为胞质受体构建重构胚，并经体外培养，发现不同的胞质受体均能支持重构胚的早期发育，但受体胞质对重构卵的重构胚早期发育速度和卵裂率均有显著影响。

The primary sporogenous cells divided into the second sporogenous cells which would turn into microspore mother cells in a microsporangium. The cytokinesis in meiosis of the microspore mother cells was of modified simultaneous type, forming the decussate, isobilateral or T-shaped tetrads.

初生造孢细胞分裂形成次生造孢细胞，次生造孢细胞再转化为小孢子母细胞，小孢子母细胞减数分裂的胞质分裂为修饰性同时型，四分体排列方式为交叉型、对称型或"T"型，成熟花粉粒二细胞型，开花时散出。

According to the changes of the structure of chromatin and the morphology of cell, the process of the typical spermatogenesis of Cipangopaludina chinensis can be divided into five stages: spermatogonium, primary spermatocyte, secondary spermatocyte, spermatid and mature sperm.

中国圆田螺典型精子发生时，根据染色质和细胞形态的变化，可将其发生分为以下五个时期：精原细胞、初级精母细胞、次级精母细胞、精细胞和成熟精子。

With DGD method, the electron microscopy observation has provided new information on the process of chromatin migration. That is: Firstly, the nucleus moved toward cell wall and a picture characteristic of synizesis stage of meiosis appeared; Secdonly, the transmigration of chromatin occured through CC, and a more extensive region constituted of nuclear skeleton left behind chromatin, which was named as"clear spaces" under light microscopy; Thirdly, at the late stage of synizesis, most of chromatin had gone into adjacent cells through CC and fused into a whole, and the nuclear skeleton left in the former cell gradually mixed with cytoskeleton in cytoplasm.

DGD—包埋去包埋电镜观察表明：百合花粉母细胞中有类似核纤层的结构存在；在染色质穿壁运动过程中，首先是细胞核向细胞壁靠拢，并可留下瞬间的运行轨迹——细胞核后方出现只有细胞骨架而少有细胞器的区域；其后是染色质开始穿壁，在穿壁染色质后方细胞核内出现无染色质仅有密集的核骨架的区域，到了染色质穿壁后期，大部分核物质都已穿至相邻细胞，并彼此融合，而残余在原穿出细胞中的核骨架己逐渐与原细胞的胞质骨架融为一体。

This result indicates that WT1 gene plays an important role in differentiation and development of fetal kidney and may be the factor that promotes metanephric blastemal cell to differentiate into epithelial cell.

结果显示小胎龄肾组织中WT1蛋白在胚基细胞和幼稚肾小球细胞核表达而大胎龄组肾组织中WT1在肾小管细胞胞浆表达，阳性率分别为57.1％(8/14)和46.2%(6/13)，提示WT1基因在胚胎肾分化发育的过程中起着重要作用，WT1蛋白可能是促进后肾胚基细胞向上皮细胞分化的调控因子，其表达在时间上和空间上都受到严格的调控，WT1的表达异常可能导致胚基细胞分化停滞。17例肾母细胞瘤WT1蛋白表达阳性率为41.2%(7/17)，阳性部位在胚基型和上皮型肿瘤细胞核，表达部位和阳性率与早期胚胎肾相似，其中间质型肾母细胞瘤均为阴性，胚基型和上皮型肾母细胞瘤阳性率70%(7/10)，两组间阳性率有显著差异。

The primary sporogenous cells divided into the second sporogenous cells which would turn into microspore mother cells in a microsporangium. The cytokinesis in meiosis of the microspore mother cells was of modified simultaneous type, forming the decussate, isobilateral or T-shaped tetrads.

初生造孢细胞分裂形成次生造孢细胞，次生造孢细胞再转化为小孢子母细胞，小孢子母细胞减数分裂的胞质分裂为修饰性同时型，四分体排列方式为交叉型、对称型或&T&型，成熟花粉粒二细胞型，开花时散出。

Myoid/myofibroblastic areas in DFSP possibly represents the hyperplasia of stromal myofibroblasts, rather than true myofibroblastic differentiation of the neoplastic cells.

DFSP中的肌样／肌纤维母细胞性分化可能是肿瘤间质中肌纤维母细胞增生的结果，并非代表了瘤细胞的真性肌纤维母细胞性分化。

About 48%(90/194) were below 29 years of age. Ninety-nine of 194 cases had either radiotherapy alone or supplementary treatment with radiotherapy following surgery. We analyzed multiple prognostic factors. The factors of importance included postoperative radiotherapy; female in glioblastoma multiforme; the primary tumor dose above 5000 rads and/or whole brain irradiation in malignant astrocytoma and glioblastoma multiforme; the primary tumor dose above 4500 rads in ependymoma, medulloblastoma and oligodendroglioma; and spinal axis irradiation above 2000 rads in ependymoma and medulloblastoma that produced a better prognosis than in other groups.

男与女之比例为2.7比1，年龄分布自1至81岁，平均年龄33.3±19.3岁，中值年龄为34岁，29岁以下占48%(90/194)，其中99例单独或手术后辅以放射腺治疗，分析同种脑瘤的预后因素，其中多形神经胶母细胞瘤有手术后放射线治疗或女性，多形神经胶母细胞瘤合并星细胞瘤原发肿瘤剂量5000雷得以上或曾全颅腔照射，神经管胚细胞瘤，间胶质母细胞瘤，及室管膜瘤原发肿瘤剂量4500雷得以上，室管膜瘤及神经管胚细胞瘤有脊轴照射2000雷得以上预后均佳，且统计上有显著的差异。

Some cell dropped into the cavity and became free. Thrombosis or part organization could be seen. The internal elastic layer became thin, disappear or broken. In internal and middle layer existed fibroblasts, fibrocytes and collagen. Some of the wall indicated hyaline change, soomth muscle cell decreased greatly. The massive inflammatory cells invaded the middle and external layer. There were many foam cells in the capsule tissue. Cytoplasm was filled with fatty tissue and cholesterol. some cavities were full of thrombosis. Some thrombosis was fibrosis, the bottom was organization. The surface of the thrombosis existed red blood cell and librae.(2)Elatic fibrila staining: the internal elastic menbrane almost completely disappeared, the intact internal elastic menbran could be seen in the new small vessels.

动脉瘤管壁厚薄明显不均，全层或局部区域显著变薄向外膨出，内皮细胞空泡变性或坏死脱落，部分内皮细胞剥离并突入管腔成游离状，可见血栓形成及部分血栓机化；内弹力板变薄、消失或突然中断；在内膜及中膜部位主要为纤维母细胞、纤维细胞和大片胶原；部分动脉瘤壁呈均质状玻璃样变，平滑肌细胞明显减少；中膜和外膜可见大量的炎性细胞浸润；瘤壁组织有纤维母细胞、纤维细胞、大片胶原成分及较多泡沫细胞，胞浆内充满脂类物质及胆固醇结晶；部分动脉瘤腔内充满血栓，有的血拴已经纤维化，血栓基部机化，血栓表面有红细胞和纤维素。

Methods: Oocytes in the GV stage were separated from ovary by squeezing method. In mouse germinal vesicle GV stage, the expression of ATP8 gene in the mitochondria in the single oocyte was detected by RT-PCR, in which, cDNA was synthesized with two methods: one was the single GV-stage oocyte directly to be placed RT, the other was to perform RT after eliminating mtDNA and nucleus DNA with the EeoR Ⅰ enzyme and Dnase. And the product of RT-PCR was cloned and sequenced.

应用挤压法从卵巢中分离获得生发泡期(germinal vesicle， GV)卵母细胞；用RT-PCR检测GV期单个卵母细胞中ATP8基因的表达：其中cDNA的合成分两种方法进行：一是将GV期单个卵母细胞直接进行RT合成cDNA，二是先用DNA酶加EcoR Ⅰ酶祛除mtDNA和核DNA后再进行RT；回收产物构建克隆质粒并测序。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失