母细胞化的

Results In the medium and high dose F0 groups, it was observed that the atrophy and incrassation of seminiferous tubule, decrease of spermatogenesis, hyperplasia of interstitial tissue, especially in high dose groups spermatozoon abnormality and nucleolus concentration in the rats testis after DU ingestion for 14 months. The changes became more severe with the prolongation of DU ingestion. Such changes occurred in filial rats (F1) after DC ingestion for 5 months. In the medium and high dose F0 groups, it was observed that a little atrophy of kidney glomerulus, hyperplasia of interstitial tissue after DC ingestion for 14 months, and kidney glomerulus fibrosis happened after DC ingestion for 20 months, such changes occurred in filial rats (F1) after DC ingestion for 5 months In the medium and high dose F0 groups, splenic germinal center and periarterial lymphatic sheaths were hyperplasia , companies with lymphopoiesis after DC ingestion for 7 months, splenic white pulp became more small and sparse after DC ingestion for 20 months.

结果 F0代的中、高剂量组大鼠摄入贫铀14个月后可见雄性的精曲小管萎缩，管壁增厚呈空虚网状，生精细胞层次减少，间质细胞增生，但仍见有精子生成；高剂量组可见到精子呈异型性改变，细胞核浓缩深染，且随着摄入时间延长改变愈趋明显；F1代大鼠摄入贫铀5个月后就有上述改变且更为严重。F0代中、高剂量组大鼠摄入贫铀14个月后肾小球轻度萎缩，间质增生明显，20个月时肾小球萎缩纤维化；F1代大鼠摄入贫铀5个月后就有上述改变。F0代中、高剂量组摄入贫铀7个月时脾脏生发中心和淋巴鞘增生，淋巴母细胞增生活跃，20个月时脾小体减少，生发中心稀疏；F1代大鼠摄入贫铀早期和晚期有类似改变。F0和F1代高剂量组摄入贫铀早期肝脏有炎症细胞浸润，晚期骨髓有核细胞减少，脂肪细胞增加。

The results indicated that spindles, chromosomes and microfilaments of porcine oocytes at different stages were nonreversible damaged obviously followed exposion to freezing medium or vitrification, which may be the main reason affecting the maturation, fertilization and development of oocytes.

本研究结果表明，猪GV期和MⅡ期卵母细胞经冷冻保护剂处理或玻璃化冷冻保存后，都明显造成了纺锤体、染色体和微丝不可逆的损伤，可能是影响卵母细胞成熟、受精与发育的重要原因。

In this study, we have identified and characterized a histone H2A variant h2af1o in fish, and revealed its oocyte-specific expression pattern during oogenesis and embryogenesis.

他们在筛选到一个卵母细胞核心组蛋白H2A变体cDNA的基础上，首先分析确定了其卵母细胞特异表达的模式，接着观察到该蛋白在卵子发生过程中的磷酸化修饰作用。

Previous studies have demonstrated that primary spermatocyte can undergo meiotic divisions in MI oocytes and the resulting embryos can develop to term. However, the success rate is very poor.

已经证明初级精母细胞在显微融入MI卵母细胞后可以完成减数分裂并且产生的胚胎可以发育到期，只是发育率还非常低，作为一种形式的核移植，细胞周期的同步化处理对于胚胎的发育具有重要意义。

The observation showed that sterile plants and fertile plants in the pollen mother cell stage is not very different, when microspore mother cells of male sterile plants enter meiosis period, it could form normal tetrad cell , tetrad cell is normal in early development stage, In the uninucleate microspore stage , tapetal cells were vacuolization, the microspores were extruded to the middle by tapetal cells.

本研究利用石蜡切片法，结合光学显微镜技术对樱桃萝卜核质互作雄性不育系的不育株和对应保持系可育株的花药发育过程进行细胞形态学观察，观察结果表明：不育株与可育株在花粉母细胞时期差别不大，不育株的花药小孢子母细胞进入减数分裂期后，可以正常的形成四分体，四分体前期发育正常，而在单核小孢子期绒毡层细胞出现液泡化，绒毡层细胞向中间挤压小孢子。

Results: The study showed that the earliest stromal nodules were fibrous, and with the dual differentiation of myofibroblasts toward both smooth muscle cells and fibrous cells, they developed into fibromyomatous nodules and muscular nodules. The results of reclassifying the 55 nodules immunohistochemically were that 23(42%) was fibrous, 28(51%) fibromuscular nodules and 4(7%) muscular.

结果：结合HE切片和免疫组化结果发现前列腺间质结节的早期为幼稚的纤维性结节，随着结节内肌纤维母细胞向平滑肌细胞和纤维细胞的双向分化，间质结节可继续演化为纤维肌性结节和肌性结节。55个结节按免疫组化分类，纤维性结节占42%，纤维肌性结节占51%，肌性结节占7%。

Methods: Immunohistochemical study of CD133 expression: 55 cases osteosarcoma conventional paraffin-embedded tissues specimens come from Pathology Department Qilu Hospital Shandong University, including 4 cases parosteal osteosarcoma, 13 cases fibroblast-type osteosarcoma , 12 cases chondroblastic osteosarcoma and 26 cases Osteoblastoma osteosarcoma.

免疫组化研究CD133的表达：取自本院病理科的骨肉瘤常规石蜡包埋组织蜡块标本55例，其中骨旁骨肉瘤4例，纤维母细胞型骨肉瘤13例，软骨母细胞型骨肉瘤12例，骨母细胞型骨肉瘤26例，切成3μm的组织切片备用。

A23187 and OA could parthenogenetically activate Urechis unicinctus oocytes and activate MAPK, indicating that the effect of fertilization on GVBD and activation of MAPK was mediated by rising in intracellular free calcium, and phosphorylation of some proteins was enough to activate the oocytes.

钙离子载体A23187和OA可以孤雌活化单环刺螠卵母细胞并激活MAPK。说明：①受精是通过钙离子的内流来激活卵母细胞并使MAPK活化的。②某些蛋白质的磷酸化状态对减数分裂是必要而且在一定程度上是充分的。

The result is consistent with previous studies. Whereas, tapetal cells of sterile anthers were disintegrated since tetrad cells were disjoined, indicating that disintegration of tapetal cells is earlier occurred in sterile anther than that in fertile anther. DNA ladder revealed that the cleavage of nuclear DNA into oligonucleosome-sized fragments to

结果表明，在花粉母细胞减数分裂后的四分体解离时期，不育花药的绒粘层细胞较可育花药提前开始裂解；在不育系S-Mo17rf3rf3花药和花粉S-(rf3)中均明显出现PCD过程的DNA片段化以及线粒体细胞色素C外渗的现象，证明了玉米CMS-S的花粉败育与花药绒粘层细胞的提前凋亡和小孢子细胞的细胞程序性死亡有关。

The histopathologic changes included lymphocyte and monocyte infiltration,hyperplasia of synovial cells and small vessels,interstitial fibrosis,hyaline degeneration and cartilaginous metaplasia.The immunohistochemical observations showed that the high expressions of IL-1 and IL-6 were significantly different between the pathologic plicae and the control groups（P＜0.01）.The positive expressions of IL-1 and IL-6 were the synovial cells and monocyt-lymph cells in the pathologic synovial plicae.The positive expressions of MMP-1 and TIMP-1 have significant difference between the experiment and control group（P＜0.01,P＜0.05）.The expression of MMP-1 was positive in synovial lining cell,monocyte,fibroblast,endothelial cell in small vessel and chondrocyte.The TIMP-1 expression was detected in the synovial lining cells and a small quantity fibroblast.

结果 正常滑膜皱襞和病理性滑膜皱襞在滑膜细胞增生及小血管增生、间质纤维化及玻璃样变、软骨化生组织学改变方面，差异均有显著性（P＜0.01）；IL-1、IL-6在病理性滑膜皱襞内的增生滑膜细胞、单核及淋巴细胞和在正常滑膜皱襞内的表达差异均有显著性（P＜0.01）； MMP-1、TIMP-1在病理性滑膜皱襞和正常皱襞内的阳性表达，差异具有显著性（P＜0.01，P＜0.05），MMP-1在增生滑膜衬里层细胞、单核和纤维母细胞、血管内皮细胞和软骨化生的软骨细胞呈阳性表达；TIMP-1只在滑膜衬里层细胞和少量纤维母细胞有表达。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。