母细胞

The duration and temperature during ovary transportation and handling might, therefore, affect the recovery of culturable COCs, chromatin configuration and developmental competence of oocytes.

在具体的实施过程中，从屠宰场向实验室运输卵巢、卵母细胞培养前操作等都需要一定的时间，这可能会对卵母细胞的回收率、卵母细胞成熟质量、甚至受精后的发育产生不良影响。

After that , the comparison of the fertilizable competences of ICSI and IVF on cryopreservated oocytes was studied in this article , in order to establish an optimized method to improve the fertilizable competences of oocytes up to the level of embryos .

和胞浆内单精子注射两种方法对玻璃化冷冻卵母细胞的受精能力进行了比较研究，旨在建立能够进一步提高牛冷冻卵母细胞受精能力的改进方案，使卵母细胞冷冻技术上升到与胚胎冷冻同样成熟的水平。

Rising of fertilization membrane during induced maturation indicates insemination is not necessary to form the fertilization membrane. The oocyte will not become fertilizable until GVBD has happened and can still be fertilized after the second polar body extrusion.

卵母细胞只有在生发泡破裂之后才具有受精能力，从生发泡破裂到第二极体排出前的整个减数分裂过程中，卵母细胞都可以受精，但是只有在第一极体排出前受精的卵母细胞才能发生卵裂。

After that, the comparison of the fertilizable competences of ICSI and IVF on cryopreservated oocytes was studied in this article, in order to establish an optimized method to improve the fertilizable competences of oocytes up to the level of embryos.1 Effects of the reducing injury methods on IVF of cryopreservated oocytesThe result of the three methods, choosing the right time oocytes, using minimum size sample vitrification and culturing after thawing, showed that these ways could partly maintained the fertilizable competences of cryopreservated bovine oocytes.

减轻损伤方案对冷冻卵母细胞IVF的影响使用选择合适时期的卵母细胞进行冷冻；采用最小样本量玻璃化冷冻方法进行冷冻；在解冻后先孵育再进行体外受精等三种方案能够部分维持或恢复冷冻牛卵母细胞IVF受精能力。

Results All the 7 cases were female, 2 cases were solitary fibrous tumor, 2 cases were myofibroblastoma, 1 case was inflammatory myofibroblastic tumour, 1 case was fibromatosis, and 1 case was low grade myofibroblastic sarcoma.

结果 7例患者均为女性，2例为孤立性纤维性肿瘤；2例为肌纤维母细胞瘤；1例为纤维瘤病；1例为炎性肌纤维母细胞瘤；1例为低度恶性肌纤维母细胞肉瘤。

To eplore the relationship between Dynein and the formation of meiosis error of oocytes, the immature mouse oocytes were in vitro cultured with a known dynein ATPase activity inhibitor- sodium orthovanadate to detect the changes of maturation rate, and semi-quantitative RT-PCR and single cell RT-PCR were performed to detect the alteration of the cyclin B1 mRNA level.

为探讨Dynein与卵母细胞减数分裂错误形成的关系，采用小鼠卵母细胞体外培养成熟技术和半定量RT-PCR方法，分别检测Dynein抑制剂-原钒酸钠对卵母细胞成熟率和cyclin B1基因转录水平的影响。

Objective:To study the diagnostic and differential criteria for benign and malignant osteoblastoma.

骨母细胞瘤临床病理学研究李亦民丁彦青徐莉摘要探讨良性骨母细胞瘤和恶性骨母细胞瘤的诊断和鉴别诊断标准。

Methods: Immunohistochemical study of CD133 expression: 55 cases osteosarcoma conventional paraffin-embedded tissues specimens come from Pathology Department Qilu Hospital Shandong University, including 4 cases parosteal osteosarcoma, 13 cases fibroblast-type osteosarcoma , 12 cases chondroblastic osteosarcoma and 26 cases Osteoblastoma osteosarcoma.

免疫组化研究CD133的表达：取自本院病理科的骨肉瘤常规石蜡包埋组织蜡块标本55例，其中骨旁骨肉瘤4例，纤维母细胞型骨肉瘤13例，软骨母细胞型骨肉瘤12例，骨母细胞型骨肉瘤26例，切成3μm的组织切片备用。

The results of the determinating the activity of the CA in vitro showed that the CA of the water—fed individuals were in activing statics after eclosion. Because of the apperence of the Vg in haemolymph and the deposition of the vitellin in oocytes in the unfed females, it was shown that the CA in such individuals were in activiting statics, too. Again because in the water—fed females which did not lack the feeding action the activity of the CA showed dropping tendency gradually still, it could be said that the activiting of the CA was possibly effected by the nutrition but it was never related to the adult feeding action. In another respect, the brain of the female adult had not significant effect on the activiting CA. The oocytes could depositing vitellin in individuals removed brain tissue on the day of eclosion, which showed that the activting of CA was not concerned with brain.

CA活性的体外测定说明饲水个体的CA同样具有活性，不取食个体血淋巴中Vg的存在以及卵母细胞中卵黄的沉积表明，在不取食的个体中CA同样具有活性，所以CA的活化与成虫期的取食动作无关，与成虫是否取食花蜜也无太多的联系，但是CA活性的维持可能受成虫期营养的影响，如果以卵母细胞发育和CA体积大小作为CA活性的指标，则饲水个体CA的活性在后期受到了影响，说明饲水不能替代取食蜂蜜来维持成虫期CA的活性；成虫期脑对CA的活化无明显作用，羽化当日摘除脑的个体，其卵母细胞仍然可以沉积卵黄，说明CA的活化与脑无关，而是受某种不确定的因子的影响；用抗JH物质—早熟素—Ⅱ以10ug／虫的剂量在颈部点滴处理雌虫，对Vg的发生和卵巢的发育具有一定的迟缓作用，其原因有待于进一步的研究。

The results showed that the expression of calm3 and trm112l genes had no remarkable differences in oocytes and 8-cells stage embryos, but were significantly different in blastocysts. It was supposed that these two genes were possibly maternal genes, and they might be involved in oocyte maturation and zygotic genome activation.

结果显示，在卵母细胞和8细胞期胚胎，基因calm3和trm112l的表达量无显著性差异，而在囊胚期的表达量与前两个时期的表达量存在显著性差异，推测这两个基因在牛胚胎发育过程中的表达可能属于母型调控，提示该基因在卵母细胞成熟和合子基因激活中起一定作用。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷