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母细胞

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All expression of c-kit in oogenesis of Acrida cinerea may be related with the growth and mature of oocyte, as well as the beginning of vitellogenesis.

母细胞的第六阶段及第七阶段的弱阳性反应主要存在于卵母细胞与滤泡细胞之间,可能与卵母细胞的成熟有关。

Intronodal palisaded myofibroblastoma is a kind of distinctive clinicopathological myofibroblastoma. It originates from myofibroblasts or specialized smooth-muscle cells.

栅状肌纤维母细胞瘤是一种具有独特临床病理特征的肌纤维母细胞瘤,该肿瘤来源于肌纤维母细胞或特殊平滑肌细胞亚型。

Result The spindles of oocytes were abnormal at 25℃ for 20 min, and disappeared at 25℃ for 30 min or at 4℃ for 10 min. 5, 10, 20 and 40 μg/ml Cytochalasin B for 2 h had no effects on the spindles of the oocytes. The spindles of oocytes were abnormal in 20 μmol/L colchicines for 1 h, and disappeared in 40 μmol/L colchicines for 1 h. The structure of spindles were perfect for 1-12 h when the oocytes were fixed in 4% paraformaldehyde.

结果]卵母细胞在25℃、20 min时纺锤体异常,25℃、30 min或4℃、10 min时纺锤体缺失;5、10、20和40μg/ml细胞松弛素B作用2 h未对纺锤体产生影响;卵母细胞在20μmol/L秋水仙素中作用1 h纺锤体异常,40μmol/L时作用1 h纺锤体缺失;在4%多聚甲醛中4℃条件下,卵母细胞纺锤体结构以固定时间为1~12 h效果较好。

Osteosarcoma ; Osteoblast ; Chondroblast ; Fibroblast ; Primitive mcsenchymal cell ; Multinucleated giant cell

骨肉瘤;骨母细胞;软骨母细胞;纤维母细胞;原始间叶细胞;多核巨细胞

Hormone level in maturation medium affected cumulus expansion of COC, but cumulus expansion became less dependent on hormone level with time of culture. Porcine immatured oocytes, granulosa cells from small antral follicles and cumulus cells of immatured oocytes secreted cumulus-expansion enabling factors. Granulosa cells from 3-6mm follicles did not produce CEEF, but there was some CEEF in the follicular fluid of this sized follicles.

激素水平对卵丘扩展有影响,但随培养时间的延长,对激素的要求有逐步减少的趋势;猪卵丘扩展不依赖于卵母细胞,卵母细胞核成熟也与卵丘扩展无关;GV期卵母细胞、小腔卵泡内颗粒细胞和成熟卵的卵丘细胞都有较高的分泌CEEF能力;3-6mm卵泡的壁颗粒细胞不能分泌CEEF,但3—6mm卵泡的卵泡液中有CEEF。

Hormone level in maturation medium affected cumulus expansion of COC,but cumulusexpansion became less dependent on hormone level with time of culture.Porcine immaturedoocytes,granulosa cells from small antral follicles and cumulus cells of immatured oocytessecreted cumulus-expansion enabling factors.Granulosa cells from 3-6mm follicles did notproduce CEEF,but there was some CEEF in the follicular fluid of this sized follicles.Sun xingshen, Animal Histology and Embryology

激素水平对卵丘扩展有影响,但随培养时间的延长,对激素的要求有逐步减少的趋势;猪卵丘扩展不依赖于卵母细胞,卵母细胞核成熟也与卵丘扩展无关;GV期卵母细胞、小腔卵泡内颗粒细胞和成熟卵的卵丘细胞都有较高的分泌CEEF能力;3-6mm卵泡的壁颗粒细胞不能分泌CEEF,但3-6mm卵泡的卵泡液中有CEEF。

Myoid/myofibroblastic areas in DFSP possibly represents the hyperplasia of stromal myofibroblasts, rather than true myofibroblastic differentiation of the neoplastic cells.

DFSP中的肌样/肌纤维母细胞性分化可能是肿瘤间质中肌纤维母细胞增生的结果,并非代表了瘤细胞的真性肌纤维母细胞性分化。

About 48%(90/194) were below 29 years of age. Ninety-nine of 194 cases had either radiotherapy alone or supplementary treatment with radiotherapy following surgery. We analyzed multiple prognostic factors. The factors of importance included postoperative radiotherapy; female in glioblastoma multiforme; the primary tumor dose above 5000 rads and/or whole brain irradiation in malignant astrocytoma and glioblastoma multiforme; the primary tumor dose above 4500 rads in ependymoma, medulloblastoma and oligodendroglioma; and spinal axis irradiation above 2000 rads in ependymoma and medulloblastoma that produced a better prognosis than in other groups.

男与女之比例为2.7比1,年龄分布自1至81岁,平均年龄33.3±19.3岁,中值年龄为34岁,29岁以下占48%(90/194),其中99例单独或手术后辅以放射腺治疗,分析同种脑瘤的预后因素,其中多形神经胶母细胞瘤有手术后放射线治疗或女性,多形神经胶母细胞瘤合并星细胞瘤原发肿瘤剂量5000雷得以上或曾全颅腔照射,神经管胚细胞瘤,间胶质母细胞瘤,及室管膜瘤原发肿瘤剂量4500雷得以上,室管膜瘤及神经管胚细胞瘤有脊轴照射2000雷得以上预后均佳,且统计上有显著的差异。

This experiment first studied in the mature nutrient medium to increase the hormone to the porine ovocyte in vitro mature process in the chromosome configuration and the mature rate influence, by explored the porine ovocyte in vitro mature rule, enhanced in vitro raise maturity, was the ROSI technology system preparation high quality ovocyte; Then has carried on the experiment in view of the porine round sperm cell in vitro separation, through to the ROSI operation and ICSI (Intracytoplasmic sperm injection, the comparison which ICSI) operated studies has affected the ROSI operation the factor.

本试验首先研究了成熟培养液中添加激素对猪卵母细胞体外成熟过程中染色体构型和成熟率的影响,以探索猪卵母细胞体外成熟规律,提高体外培养成熟度,为ROSI技术体系准备优质卵母细胞;然后针对猪圆形精细胞的体外分离进行了试验,进而通过对ROSI操作和ICSI(Intracytoplasmic sperm injection,ICSI)操作的比较研究了影响ROSI操作的因素。

The results were as follows: nanos 1 mRNA uniformly dispersed throughout the cytoplasm of oocytes at all stages. Strong expression of nanos1 mRNA was observed in oogonia and stageⅠ、Ⅱ oocytes, but the signal became weaker in later stage oocytes.

结果表明:在斑马鱼卵子发生中,nanos1 mRNA均匀分布于卵原细胞和各时期卵母细胞的胞质中;在卵原细胞和Ⅰ、Ⅱ期卵母细胞中,nanos1 mRNA的杂交信号十分强烈,而较晚期卵母细胞中信号明显减弱。

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