母细胞

The present study was conducted to investigate the effect of 6-DMAP on parthenogenetic activation rate, pronuclear formation and in vitro parthenote development of in vitro matured goat oocytes.

体外成熟的山羊卵泡卵母细胞用7%的乙醇和5μmol/L的离子霉素刺激处理后，分别置于含2 mmol/L 6-二甲基氨基嘌呤(6-DMAP)和不含6-DMAP的培养液中培养4 h，研究6-DMAP对体外成熟山羊卵泡卵母细胞孤雌激活率、原核形成以及孤雌胚体外发育潜能的影响。

Methods The ACTH, Cor, ANP and IL-6 levels in 18 children with abdominal neoplasms (12 male and 6 female, 8 cases with sacrococcygeal teratoma, 5 cases with hepatoblastoma, and 5 cases with nephroblastoma) were measured before and after operation (24 hours before operation; 4, 24, 48, and 72 hours after operation). Eighteen children were divided into the study group and the control group.

监测18例腹部肿瘤患儿（男12例，女6例；其中腹腔畸胎瘤8例，肝母细胞瘤、肾母细胞瘤各5例）术前24h，术后4、24、48、72h外周血清ACTH、Cor、ANP和IL-6水平的变化，根据患儿术后恢复情况分为研究组和对照组。

Most of EGFR in unmatured and matured oocytes was in variation, and there was obviously more expression of variation in matured oocytes than that in unmatured oocytes.

未成熟和成熟的卵母细胞中主要存在Ⅷ突变型，而野生型表达相对较少，成熟后卵母细胞中的Ⅷ突变型蛋白明显增加。

During zebrafish oogensis, vasa mRNA is expressed strongly and uniformly distributed in the cytoplasm in stage Ⅱ oocytes, followed by a distribution among vacuome in stage Ⅲ.

随着卵母细胞的生长，第Ⅲ期卵母细胞中的vasa mRNA分布在液泡之间。

The feasibility of maturing denuded buffalo oocytes in vitro was explored in this study, so as to provide a model to investigate the mechanisms of oocytes maturation.

主要探讨无卵丘水牛卵母细胞体外成熟的可行性，以便为研究卵母细胞成熟机理提供模型。

In order to establish mouse nuclei transfer technology, mouse zygote culture medium and activated conditions were optimized in vitro and enucleated method of MII oocyte were investigated in this study.

本研究通过对小鼠受精卵体外培养体系，MII期卵母细胞孤雌激活条件的筛选以及探讨MII期卵母细胞的去核方法为小鼠体细胞核移植的成功奠定基础。

Our study demonstrated that the use of 3% sucrose can give a judgment to embryo quality and then ooctyes with good quality could be selected and enucleated by this method for further micromanipulation.

所以，3％的蔗糖处理还可用于卵母细胞质量好坏的鉴别，有利于在较短时间内挑选出好的卵母细胞进行去核或进一步的操作。

In order to find an effective nuclear transfer program, this thesis investigated three factors affecting the efficiency of somatic nuclear transfer on goats: the in vivo mature time of oocytes, the component of operation liquid for oocyctes enucleating and the parthenoactivation mode of oocytes.

本实验通过改变以下试验条件：卵母细胞体外成熟时间的不同、卵母细胞去核时操作液成分的不同，来研究其对山羊核移植效率的影响，希冀寻找一个有效的核移植方案。

Enucleating the nuclei of recipient oocytes accurately is one of the key techniques in nuclear transfer research. Enucleation of mouse MⅡoocytes can be performed under an inverted microscope equipped with Normarski or Hoffmann optics.

准确去除受体卵母细胞的细胞核是核移植研究的关键技术之一，在装备有Normarski 或Hoffmann 光学镜头的倒置显微镜下可以准确去除小鼠MⅡ期卵母细胞的细胞核。

Although the technology of In Vitro Maturation is more and more maturated, the mechanism of maturation needs further study.

虽然牛卵母细胞体外成熟培养技术已趋于成熟和完善，但卵母细胞成熟机理的研究还有待深入。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。