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During denuded mice oocyte mature process in vitro, the cortical granulesmay have abnormal numbers or generation problems. This may be another reason thatmakes denude oocytes matured in vitro have a clearly deficiency on fertilization invitro.

3去除卵丘细胞的小鼠卵母细胞在体外成熟过程中,皮质颗粒可能存在继发性生成障碍或者数量异常,该结果可能是去除卵丘细胞的小鼠卵母细胞存在体外受精障碍的原因之一。

The results shown as follow: 1. The study on mice denuded oocytesin vitro maturation, in vitro fertilizationand in vitro development. This part of paper is to discuss whether the remove of granulose cells can affect the mice oocytes maturation rates, fertilization rates and development rates in vitro.

一、去除卵丘细胞的小鼠卵母细胞的体外成熟、体外受精初步研究本实验探讨了不同成分明确的成熟培养液对去除卵丘细胞的小鼠卵母细胞的体外成熟、体外受精和胚胎的体外发育的影响。

Two cases were classified as diffuse lymphoblastic lymphosarcoma and one each as nodular prolymphocytic, nodular lymphoblastic, diffuse mixed cell type lymphosarcoma, Lennert's lymphoma, mixed cell type Hodgkin's disease.

按组织学分型,弥漫性淋巴母细胞型2例和弥漫性混合细胞型淋巴肉瘤、结节性前淋巴细胞型、结节性淋巴母细胞型、Lennert氏淋巴瘤及混合细胞型何杰金氏病各1例。

The results show that, with the maturity of the gonad, the undifferentiated germ cell divided and differentiated to smaller oogonium or spermatogonia, and the oogonium or spermatogonia transformed to oocyte and sperm undergoing a series of maturation process.

摘 要 通过观察栉江珧的未分化生殖细胞,精原细胞和卵原细胞,精母细胞和卵母细胞,精子和卵子的形态结构与分布状况及核仁在卵成熟过程中的变化,对栉江珧生殖细胞发生及成熟过程的有关问题进行了探讨。

The spermatogenesis includes five stages: spermatogonium、primary spermatocyte、secondary spermatocyte、spermatid and spermatozoon.

雄体的生殖细胞发生经历精原细胞、初级精母细胞、次级精母细胞、精子细胞和成熟精子五个时期。

Its development as follows: spermatogonia → primary spermatocyte secondary spermatocyte →→→ sperm sperm cells.

其发育过程为:精原细胞→初级精母细胞→次级精母细胞→精子细胞→精子。

Under normal temperature, anthers of not fertility restoration stopped developing in the stage of archesporical cell; 1-2 comers of a few anther could differentiate and form archesporical cell, sporogenous cells and microspore mother cell, but microspore mother cell disintegrated on the first meiotic division.

常温下,未发生育性恢复的花药在孢原细胞期就停止发育;少数花药1~2个角隅处可分化形成孢原细胞,造孢细胞,花粉母细胞,但花粉母细胞在减数分裂的第一次分裂时解体。

Strong expression of nanos 1 mRNA was detected in spermatogonia and relatively weaker signal was found in primary spermatocytes but no nanos 1 mRNA expression was observed in spermatids. The results suggested that nanos 1 may play an important role in maintenance and functioning of the germline stem cell—oogonia and spermatogonia.

在斑马鱼精子发生中, nanos1 mRNA可在精原细胞和初级精母细胞中检测到。nanos1 mRNA的阳性信号在精原细胞中极为强烈,在初级精母细胞中较为微弱,而精子细胞中没有阳性信号。

Also, the intensity of immuno-positive reactions to them enhance with the development of the gonads.

免疫组织化学法也显示卵原细胞、卵母细胞、精原细胞、精母细胞和精子细胞存在睾酮和17β-雌二醇的免疫阳性物质,而且睾酮和17β-雌二醇的免疫反应强度也是随着性腺发育成熟而增强。

Studies on mouse nuclear transfer with adult male mouse ear fibroblast cells as donors In this study, C57BL/6 adult male mouse ear fibroblast cells and Kunming mouse MⅡoocytes were used as donors and recipients, respectively, to investigate the effects of different passages of donor cells and electrofusion times on the in vitro development of NT reconstructed embryos, when the oocytes were treated with 3% sucrose.

以成年雄鼠耳成纤维细胞为供体克隆小鼠的研究该研究以C57BL/6 成年雄鼠耳成纤维细胞为供体,昆明白小鼠MⅡ期卵母细胞为受体,研究了3%蔗糖处理卵母细胞去核后,供体细胞不同传代次数和电融合次数对核移植重构胚体外发育的影响。

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