母核

AIM To study the isolation and in vitro culture of rabbit s bone mesenchymal stem cells and adipose stromal stem cells and the use of both BMSCs and ASSCs cells in nuclear transfer to produce totipotent blastocysts.

目的 研究非休眠期成年兔骨髓基质干细胞和脂肪基质干细胞作为供体，以体外成熟去核兔卵母细胞作为受体进行核移植构建重组胚胎的可行性及囊胚形成率。

Unicolor. There were unequal bivalents, homologous chromosome deconjugation, chromosome brige, unequal separation, laggard chromosomes, chromosome outside nucleus, micronuclei arid so on.

unicolor小孢子母细胞减数分裂异常进行了研究，发现存在不等二价体、同源染色体早分离、染色体桥、不均等分离、滞后染色体、核外染色体、微核等。

The results showed that the tapetum of Zhenshan 97B disrupted promptly at the late uninucleate pollen stage. Whereas, the tapetum of Zhenshan 97A disaggregated at the pollen mother cell stage, the nuclear membrane and the cell membrane disaggregated, the phenomena continued to the binucleate pollen stage.

研究发现保持系绒毡层细胞在单核花粉晚期才开始迅速解体，而不育系绒毡层细胞在花粉母细胞时期就开始出现核膜、细胞膜解体，此过程持续到二核花粉时期。

The main research achievements on oogenesis and embryogenesis in mammals are described in this review,including the resumption of meiotic division;activation of MⅡ oocyte;development of parthenogenetic,gynogenetic,androgenetic and reconstituted embryos;and the genetic and reproductive potentials of chromosomes within the first and the second polar body.

本研究将论述哺乳动物卵子发生和胚胎发生的最新研究进展，包括：减数分列的启动， MⅡ期卵母细胞的激活，孤雌胚、雌核胚、雄核胚和重组胚的发育以及第一与第二极体染色体的遗传和生殖潜力。

A single donor cell was injected into the perivitelline space of enucleated oocyte and fusion was induced with electric pulses. Reconstructed oocytes were activated using ionomycin (5 M,5min) and 6-dimethylaminopurine (6-DMAP,2mM,5h).

将供核细胞注射到去核卵母细胞的卵周隙，经电融合获得重构胚，再经离子霉素(Ionomycin，5μM，5min)和6-二甲基氨基嘌呤(6-DMAP，2mM，5h)处理激活。

Enucleated metaphase Ⅱ oocyte can support reprogramming of transferred nucleus and further development to term.

许多实验证明去核的MⅡ期卵母细胞可支持移入的核进行重新编程并发育到出生。

Enucleated rabbit oocytes would be used as recipients were activated by electric stimulus before nuclear transfer.

兔去核卵母细胞先经单个电脉冲进行活化刺激，然后进行核移植。

The aim of this study was designed to explore the possibility oftransferring bovine embryo blastomeres into buffalo nucleated oocytes.

本实验探讨了将体外培养成熟的水牛卵母细胞去核后，作为体外受精生产的黄牛胚胎细胞核移植的受体进行种间核移植的可能性。

It is known that one of the important mechanisms for epigenetic reprogramming is DNA methylation.

目前的研究认为这些异常是由于体细胞核在去核卵母细胞中重编程错误造成的，导致克隆牛胚胎的发育潜能降低。

Megaspore mother cells undergo meiosis, which results in the formation of 4 megaspores in a row. The chalazal-most cell develops into an 8-nuclei embryo sac after 3 rounds of mitosis. A mature embryo sac is produced after cellularization and nuclear migration.

结果表明：1大孢子母细胞经过减数分裂形成直线排列的4个大孢子，其中珠孔端的3个大孢子退化，合点端的大孢子进行3次有丝分裂，形成八核胚囊， 8个核迅速细胞化，最终发育为成熟胚囊。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。