母核

The three types of reconstructed embryos(reconstructed embryo of goat-rabbit, goat-bovine and goat-goat) were produced respectively by nuclear transfer using goat ear firbroblast cells as donors and rabbit, bovine and goat ooctyes as recipients, and the factors of cytoplast environments influencing cloned embryo development in vitro, cytoplast effect in nuclear transfer, the methods of transferring cloned embryo and the effects of pregnancy factor on implantation of cloned embryo were studied in this experiment, in order to supply basis for solving the difficulty of implantation of inter-species cloned embryo in recipient and improving the efficiency of cloning.

本试验以波尔山羊耳成纤维细胞为供体，分别以牛、羊、兔的卵母细胞为受体进行体细胞核移植，构建了山羊-兔、山羊-牛和山羊-山羊三种重构胚。研究了不同的胞质环境对重构胚体外发育的影响与核移植中的胞质效应，并对异种克隆胚胎采用了去透明带移植和配种后再移植的方法，探讨移植方法和妊娠因子对异种克隆胚胎在受体动物体内发育的影响，旨在为解决异种克隆胚移植不易着床发育的难题和为提高动物克隆效率提供理论依据。

In mouse, the position of first polar body (PB1) and MⅡ nucleus or spindle is moveable and thus it is difficult to enucleate beneath the PB1. In this study, the 3% sucrose treatment was prepared for enucleation.

在小鼠的卵母细胞中，第一极体（PB1）与MⅡ期核或纺缍体的位置是不固定的，因此导致了去核的困难。

Effects of in vitro maturation duration and enucleation time of buffalo oocytes on the NT embryo development were studied.

探讨了受体卵母细胞的成熟和去核时间对水牛核移植效果的影响。

The appropriate electric fusion parameters for nuclear transfer of porcine somatic cells were investigated using in vitro matured porcine oosytes as recipients and granulose cells as donors in this study.

本研究用体外成熟42~46 h的猪卵母细胞为核移植受体，猪颗粒细胞为核移植供体，对猪体细胞核移植的电融合参数进行了探讨。

Following the puncture of zona pellucida with two holes by injection pipette that contained donor nuclei or cells, the injection pipette was pulled back to the perivitelline space while the negative pressure was increased in the holding pipette until the polar body and karyoplasm were wiped off completely. Then a reconstructed embryo was completed by the direct injection of the donor nucleus or cell without pulling out the injection pipette.

首先以预先吸有细胞核或细胞的注射针在固定于持卵针上的卵母细胞透明带上穿刺两个孔，然后一边缓慢地将注射针回拔至卵周隙中，一边逐渐增加持卵针中的负压，直至极体与目标核质被完整吸入持卵针中而完成去核，最后在不拔出注射针的情况下直接注射细胞核或完整细胞进而完成重构胚的构建。

At the small growth stage, three kinds of the sex steroid receptors usually were localized in the cytoplasm or nuclear membrane of oogonia and early primary oocyte, and localized in the cytoplasm and nucleoplasm of oocytes at the large growth stage and mature stage.

在小生长期，3种受体通常定位在卵原细胞和早期初级卵母细胞的胞质或核膜；在大生长期和成熟期，则定位在胞质和核质。

We all know that mother wavelet function can create waveletframe, and the kernel function with wavelet frame can build a group of basis insquare integral space only by translation.

由于母小波函数可以生成小波框架，我们用小波框架来构造核函数，而采用该核函数的SVM，能够逼近平方可积空间中的任意函数。

Cytomixis was observed at synizesis of prophase Ⅰ in this experiment, and MN in cytoplasm and in nucleus was noticed to distribute in these granules which migrated from one PMC into its neighboring cell.

在凝线期的花粉母细胞中观察到细胞融合现象，有胞质或核内微梁骨架与穿壁转移的胞质小球和核小球内骨架相连。

The preimplantation development competences of somatic cell nuclear transfer embryos reconstructed with enuleated goat Metaphase II oocytes matured in vivo and whole cells derived from adult fibroblasts of several mammalian species (goat, boer goat, bovine, tahr, panda) and human patient were evaluated.

研究去核山羊体内成熟的M II期卵母细胞与异种成年的哺乳动物(包括山羊、波尔山羊、牛、塔尔羊、熊猫)及人的成纤维细胞融合形成的体细胞核移植胚胎着床前的发育能力。

LH plaies a role to delay the occurrence of germinal vesicle, prolong the maturation duration of cytoplasm, enhance the nuclear and cytoplasm synchronization of ovine oocytes matured in vitro, and finally improve the potentiality of their in vitro development.

LH可能通过延迟生发泡破裂和促进胞质成熟的方式，使绵羊卵母细胞的核质成熟趋于同步，有助于绵羊卵母细胞的体外成熟以及受精后的进一步发育。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。