母核

The rate of Flow cytometry to analyse retinoic acid haploid is 19.3%.The sperm-like cells achieved could activate bovine oocytes by sperm injection, and the development rate of blastosphere was 20.36%. Conclusions SSCs of newly -born mice can be induced and differentiated into sperm-like cells and are also capable of activating oocytes.

本实验通过体外诱导新生小鼠SSCs分化为精子样细胞，探讨不同诱导方法的诱导效率；采用显微注射法将精子样细胞注射到卵母细胞和去核卵母细胞，探讨其体外重构胚的发育能力，为SSCs体外分化研究打下基础。

Results Myoid/myofibroblastic differentiation occurred most commonly in fibrosarcomatous DFSP. It was recognized histologically as peripherally distributed or randomly scattered small eosinophilic nodules or short bundles, which were composed of bland spindle cells, closely resembling smooth muscle cells or myofibroblasts.

结果 肌样／肌纤维母细胞性分化多出现在纤维肉瘤型DFSP中，表现为肿瘤周边部或肿瘤内散在性分布的深嗜伊红色小结节或短条束，由梭形细胞组成，细胞多无异型性，核分裂象也罕见，形态上似平滑肌细胞或肌纤维母细胞。

Follicular oocytes had developed to the stage of GV, GVBD, M I, MII after 0 h, 4 h, 14 h and 24 h of culture, respectively, judged by morphological cretirea of oocyte chromosome stained with orcein.

根据地衣红染色，对山羊卵母细胞的核染色体的形态观察发现，直径为2-3mm的卵泡其卵母细胞在体外分别培养0、4、14和24小时即可发育到GV、GVBD、MⅠ、MⅡ期。

AIM: To explore optimal superovulating methods and parthenogenetic activation conditions in rabbits.

目的：探索兔卵母细胞的最佳超排方法及孤雌激活的条件，为人兔异种核移植提供充足的兔卵母细胞。

This antibody cross-reacted with the Vasa signals in the perinuclear nuage-like structures of young oocytes and some oocytes bearing early and mid-vitellogenesis.

实验结果显示在刚分化的卵母细胞以及在卵黄生成早、中期的卵母细胞，较集中的Vasa讯号出现在核膜周围。

Akoya pearls are bead-nucleated culture pearls that are produced by the Pinctada fucata martensii, also known as the akoya oyster.

海水珍珠珠，有核珍珠的文化是由合浦珠母贝珠母生产，也被海水牡蛎众所周知的。

Our study demonstrated that the use of 3% sucrose can give a judgment to embryo quality and then ooctyes with good quality could be selected and enucleated by this method for further micromanipulation.

所以，3％的蔗糖处理还可用于卵母细胞质量好坏的鉴别，有利于在较短时间内挑选出好的卵母细胞进行去核或进一步的操作。

Enucleating the nuclei of recipient oocytes accurately is one of the key techniques in nuclear transfer research. Enucleation of mouse MⅡoocytes can be performed under an inverted microscope equipped with Normarski or Hoffmann optics.

准确去除受体卵母细胞的细胞核是核移植研究的关键技术之一，在装备有Normarski 或Hoffmann 光学镜头的倒置显微镜下可以准确去除小鼠MⅡ期卵母细胞的细胞核。

In addition, we studied the distribution of mitochondria in metaphase Ⅱ rabbit oocytes, zygotes and early embryos by two active mitochondria dyes and revealed the change of active mitochondria during early development of rabbit embryos. We also found that the distribution of microfilaments and microtubules have similar patterns to that of active mitochondria, which is an important basis for further development of animal cloning study using the recipient oocytes derived from rabbit.

此外，我们应用两种线粒体荧光染料研究了日本大耳白兔体内成熟卵母细胞、正常交配后收集的受精卵及早期体外发育胚胎的活性线粒体的位置变化，首次揭示了兔早期胚胎中活性线粒体的变化特征及其与微丝微管变化的相关性，为进一步开展以兔卵母细胞作为核移植受体的动物克隆研究打下了重要的基础。

Resembled to control oocytes, MAPK was inactivated after cytoplasts activation. However, after activation the cytoplasts showed fragmentation while pronuclei were formed in the control oocytes. This indicated that nuclei play important role in maintainence of oocytes normal morphology.

成熟胞质的活化与卵母细胞相似，在活化后MAPK被迅速灭活，只是胞质在活化处理后均发生碎裂，而卵母细胞活化后形成原核，这表明核在细胞形态维持中起重要作用。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。