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It showed that the cumulus cells did play significant role in the stimulation of nuclear maturation of porcine oocytes and subsequent development.

表明卵丘细胞对猪卵母细胞的核成熟及卵母细胞体外受精后的卵裂和早期胚胎发育,都具有明显的促进作用。

Enucleation by Spindle-View system could eliminate the effects of changing of the position on enucleation rate.

结果发现,随着卵母细胞成熟时间的延长,卵母细胞的核与PB〓的相对距离增大。

The dominant male sterility was expressed normally in the new Taigu genic sterile wheat carrying the intergenomically translocated Ms2, and the female fertility mechanism in its male sterile plants was normal as well. Observation of the chromosome configuration at meiosis of pollen mother cells of the young ears of the sterile plants showed that they were euploid plants (2n=42). No configurations different from those of the Taigu geneic sterility gene located at the original locus were noticed of the Ms2 intergenomically translocated back into the common wheat.

所获携带易组Ms2基因的新型太谷核不育小麦其显性雄性不育特性表达正常,且雄性不育株的雌性可育机制正常,对不育株幼穗花粉母细胞减数分裂期染色体构型的观察可见其为整倍体(2n=42),尚未发现回归普通小麦的易组太谷核不育基因与原位点的太谷核不育基因有不同的表型。

The formation of martensite is accomplished by the immigration of well-defined glissile interface (121)fcc type and its misfit dislocations can produce the lattice invariant deformation on the basis of phenomenal theory of martensitic crystallography, however, LID is retarded slightly after the migration of interphase (121)fcc, i.e.a thin plate-like zone exists without LID in martensite near the well-defined interface. When the temperature reduces to the Ms point, the lattice parameter of austenite matrix is √3/2 times that of the martensite without LID. This critical condition for spontaneous transformation agrees with that the stack fault energy in matrix is less than zero according to Olson and Cohen's nucleation model.

通过模型分析表明:fcc/bcc马氏体形核与长大过程是通过(121)fcc型择优界面推移进行的,界面上的错配位错可以完成马氏体晶体学唯象理论要求的点阵不变变形,但LID要稍滞后于界面迁移,即在马氏体形核与长大过程中推移界面新相一侧存在一未发生LID的新相薄区;当相变温度达到马氏体相变点Ms时,母相奥氏体与这一薄区的晶格常数比为√3/2,这一几何条件和Olson-Cohen形核模型中要求扩展位错层错区界面能γ≤0是等价的。

Previous studies have demonstrated that primary spermatocyte can undergo meiotic divisions in MI oocytes and the resulting embryos can develop to term. However, the success rate is very poor.

已经证明初级精母细胞在显微融入MI卵母细胞后可以完成减数分裂并且产生的胚胎可以发育到期,只是发育率还非常低,作为一种形式的核移植,细胞周期的同步化处理对于胚胎的发育具有重要意义。

A lot of follicular compartment cells, include cumulus cells, theca cells, granulosa cells cooperate with each other to facilitate the maturation of oocytes.

卵母细胞的成熟过程至少需要经过周围卵丘细胞、卵母细胞核以及核外细胞质等几个方面一系列的变化过程。

The cloning of mammals by somatic cell nuclear transfer requires epigenetic reprogramming of the differentiated state of donor cell to a totipotent, embryonic ground state.

由于供体核支持整个克隆动物的发育,所以当供体核被移入去核的卵母细胞后,必需进行重编程,回到胚胎发育的起始阶段。

The observation showed that sterile plants and fertile plants in the pollen mother cell stage is not very different, when microspore mother cells of male sterile plants enter meiosis period, it could form normal tetrad cell , tetrad cell is normal in early development stage, In the uninucleate microspore stage , tapetal cells were vacuolization, the microspores were extruded to the middle by tapetal cells.

本研究利用石蜡切片法,结合光学显微镜技术对樱桃萝卜核质互作雄性不育系的不育株和对应保持系可育株的花药发育过程进行细胞形态学观察,观察结果表明:不育株与可育株在花粉母细胞时期差别不大,不育株的花药小孢子母细胞进入减数分裂期后,可以正常的形成四分体,四分体前期发育正常,而在单核小孢子期绒毡层细胞出现液泡化,绒毡层细胞向中间挤压小孢子。

The giant panda-rabbit interspecies reconstructed embryos were constructed by injection of giant panda somatic cells into the perivitelline space of the enucleated oocytes and fused by electrical stimulation, and the reconstructed embryos can develop to hatched blastocyst stage in vitro. We demonstrated that the nuclear genetic materials in reconstructed blastocyst cells were the same as that of giant panda somatic cells by chromosome count and the existence of characteristic chromosome, which was confirmed by microsatellite DNA analysis later In order to determine the fate of mtDNA of giant panda-rabbit reconstructed embryos, we analyzed the cyt b sequence of embryos at 1 -cell stage, 2-cell stage, 4-cell stage, 8 cell stage, morula stage, blastocyst stage, expanded blastocyst stage and hatched blastocyst stage.

异种克隆大熊猫重构胚是通过将大熊猫成纤维细胞移入日本大耳白兔去核卵母细胞透明带下后经电融合构建的,可在体外发育至孵化囊胚阶段,我们应用一种简便、快速的异种重构胚的鉴定方法,即通过染色体数目和特征染色体的存在初步鉴定出大熊猫-兔异种重构胚的核遗传物质来源于大熊猫供核体细胞(后经昆明动物研究所通过微卫星DNA分析验证了该结果的可靠性)。

Donor cells in manipulation medium contain of 7% ethanol prior to injection were injected directly to enucleated cytoplasts.

供核细胞注核前置于含7%乙醇的操作液中,然后直接注入到去核的卵母细胞质内。

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