母核

According to the synthetic methods study of the quinolones, it is found that the synthetic method of parent ring is of wide use by the condensation from α-substituted aryl formyl-β-substituted aminoacrylic acid and the inner molecular nucleophilic substitution reaction, which is almost suitable for the quinolones preparation of different rings or different substituted groups in 〓-position.

然后，对其合成方法进行了归纳，发现：由α-取代芳甲酰基-β-取代氨基丙烯酸缩合，经分子内亲核取代反应形成喹诺酮母环的合成方法用途非常广，几乎适用于所有不同环系或〓-位是不同取代基的喹诺酮类衍生物的制备。

In the late of April of the second year, male inflorescences unbound and pollinated. When female inflorescences accepted the pollen grains, ovary development was increased in early May. With the development of either of the two ovule, funiculus, integument and nucellus were differentiated. Embryo sac mother cell came into being and the meiosis begins. Then fuctional megaspore developed into matured embryo sac that contain eight nucleuses. In the end of May, double fertilization generated.

次年，4月下旬，雄花序解螺旋散粉，雌花序开花授粉；5月上旬，雌花序授粉后，迅速增长，子房膨大，两个胚珠分化出珠柄、珠被和珠心，珠心中产生孢原细胞；5月中下旬，大孢子母细胞形成并减数分裂，功能性大孢子形成成熟的八核胚囊；5月底，发生双受精。

Results In the medium and high dose F0 groups, it was observed that the atrophy and incrassation of seminiferous tubule, decrease of spermatogenesis, hyperplasia of interstitial tissue, especially in high dose groups spermatozoon abnormality and nucleolus concentration in the rats testis after DU ingestion for 14 months. The changes became more severe with the prolongation of DU ingestion. Such changes occurred in filial rats (F1) after DC ingestion for 5 months. In the medium and high dose F0 groups, it was observed that a little atrophy of kidney glomerulus, hyperplasia of interstitial tissue after DC ingestion for 14 months, and kidney glomerulus fibrosis happened after DC ingestion for 20 months, such changes occurred in filial rats (F1) after DC ingestion for 5 months In the medium and high dose F0 groups, splenic germinal center and periarterial lymphatic sheaths were hyperplasia , companies with lymphopoiesis after DC ingestion for 7 months, splenic white pulp became more small and sparse after DC ingestion for 20 months.

结果 F0代的中、高剂量组大鼠摄入贫铀14个月后可见雄性的精曲小管萎缩，管壁增厚呈空虚网状，生精细胞层次减少，间质细胞增生，但仍见有精子生成；高剂量组可见到精子呈异型性改变，细胞核浓缩深染，且随着摄入时间延长改变愈趋明显；F1代大鼠摄入贫铀5个月后就有上述改变且更为严重。F0代中、高剂量组大鼠摄入贫铀14个月后肾小球轻度萎缩，间质增生明显，20个月时肾小球萎缩纤维化；F1代大鼠摄入贫铀5个月后就有上述改变。F0代中、高剂量组摄入贫铀7个月时脾脏生发中心和淋巴鞘增生，淋巴母细胞增生活跃，20个月时脾小体减少，生发中心稀疏；F1代大鼠摄入贫铀早期和晚期有类似改变。F0和F1代高剂量组摄入贫铀早期肝脏有炎症细胞浸润，晚期骨髓有核细胞减少，脂肪细胞增加。

Studies on mouse nuclear transfer with compacted morula blastomeres as donors In this study, C57BL/6 mouse morulae blastomeres and Kunming white mouse MⅡoocytes were used as donors and recipients, respectively, to investigate the effects of different sucrose concentration on the manipulation time of nuclear transfer, and the in vitro development of reconstructed embryos.

以致密桑椹胚卵裂球为供体克隆小鼠的研究该研究以C57BL/6 小鼠致密桑椹胚卵裂球为供体、昆明白小鼠MⅡ期卵母细胞为受体，研究了不同蔗糖浓度对核移植操作时间及重构胚体外发育的影响。

In addition, some abnormal cytology behaviors, such as univalence, multivalence, chromosome bridges, lagging chromosomes, non-equivalent division, and micro-nucleus, etc. were also observed in the meiosis of pollen mother cell.

在杉木花粉母细胞减数分裂过程中观察到一些异常细胞学行为，即单价体和多价体、染色体桥、滞后染色体、不均等分离、微核等异常现象。

The spiny eel , belonging to Perciformes, Osteichthyes, is a kind of little freshwater fish distributed in Southern China. In 1998, Our lab detected XX/XY differentiated sex chromosomes in this specie with karyotypic analysis, and then with chromosomal binding and SC observation, we identified that its two largest metacentric chromosomes were X, Y sex chromosomes in 1999. X, Y chromosomes differ each other not only in the distribution of heterochromatin but also in their shapes, which make them could be recognized directly under the light-microscope without any banding treatment.

刺鳅，属鲈形目、刺鳅科(Perciformes，Mastacembelidae)，是广泛分布于中国南方的小型淡水鱼类。1998年，我室通过核型分析，首次发现该物种具有明显分化的异形性染色体，其性别决定类型为XX／XY型。1999年，本室通过染色体显带、精母细胞联会复合体观察，再次确认刺鳅染色体组中最大的一对中部着丝粒染色体为X、Y性染色体，两者不仅已经出现结构异染色质分布上的不同，同时在外部形态上也存在明显差异，识别特征鲜明，在光镜观察下无需显带即能清晰辨认性染色体。

And at 600℃,in addition to needle carbide,there was pearlite.

在500℃时效处理时，沿晶界形核并向晶内生长的针状碳化物与母相奥氏体之间的位向关系和在晶内析出的针状碳化物的不同；以及在晶内析出的针状碳化物可以交叉生长。

Germinal vesicle breakdown and the first polar body (PB1) extrusion were observed in all groups.

观察各组生发卵泡破裂和第一极体(PB1)的形成，出现PB1的卵母细胞视为核成熟。

Results showed that the PMC meiosis of autotetraploid was similar to the diploid except some particularities. In metaphase Ⅰ, multivalent, quadrivalent, trivalent, bivalent and univalent were observed and some of the chromosomes were found not to rank on the metaphase plate and so did in metaphase Ⅱ cells. In anaphase Ⅰ and anaphase Ⅱ cells, there were lagging chromosomes, chromosome bridge and fragment. During anaphase Ⅱ and telophase Ⅱ, chromosome segregation was not synchronous or equal. At tetrad stage, dyad, triad, tetrad with micronucleus and polyad appeared.

结果表明，同源四倍体花粉母细胞减数分裂过程与二倍体相比，中期Ⅰ染色体构型复杂，有多价体、四价体、三价体、二价体和单价体；中期Ⅰ及中期Ⅱ有部分染色体没有排列在赤道板上；后期Ⅰ及后期Ⅱ出现落后染色体、染色体桥及断片；后期Ⅱ和末期Ⅱ有染色体不同步分离及不等分裂的现象；四分体时期还出现二分体、三分体、含微核的异常四分体及多分体。

The trail group which have all regulation elements of SCAP/SREBP pathway can express more fluorscence protein than the control group.［Conclusion］ The experiment provides that the curcumin can upgrade the expression of low density lipoprotein receptor by SCAP/SREBP regulation pathway. The model can be applied for quick screening of the scap ligand drugs and inverstigating the mechanism of the drugs.

1下游基因的表达可能是姜黄素调脂作用的机理之一；利用核内共注射的方法在爪蟾卵母细胞中建立的SCAP/SREBP调控系统可作为SCAP配体类药物快速筛选和作用机制研究的细胞模型。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。