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Objective Study the function of p21-activated kinase 2 in Xenopus oocyte cytokinesis.

目的 研究p21活化激酶2（PAK2）在爪蟾卵母细胞的细胞质分裂中的作用。

Although the ability to reprogramming of Kun Ming white mouse oocytes cytoplast is not effectively, reprogramming ability has improved when cystoplasts from oocytes and embryos work together.

昆明白小鼠卵母细胞胞质对体细胞核的重新编程能力较弱，而当其与正常受精卵的胞质共同作用时可较好地改善对体细胞的重新编程能力。

After treatment in M2〓 medium containing 3% sucrose, a swelling around the metaphase chromosomes could be easily found in perivitelline space and, moreover, the area of spindle, becoming transparent and taking a shape of"∞"and"O", could be well distinguished from other cytoplast.

在本研究中，采用含3％蔗糖的M2〓操作液（3SCOM）处理卵母细胞，可使纺缍体部分变为透明并呈"〓"或"O"形状，这样可使其明显区别于周围的细胞质。

The three types of reconstructed embryos(reconstructed embryo of goat-rabbit, goat-bovine and goat-goat) were produced respectively by nuclear transfer using goat ear firbroblast cells as donors and rabbit, bovine and goat ooctyes as recipients, and the factors of cytoplast environments influencing cloned embryo development in vitro, cytoplast effect in nuclear transfer, the methods of transferring cloned embryo and the effects of pregnancy factor on implantation of cloned embryo were studied in this experiment, in order to supply basis for solving the difficulty of implantation of inter-species cloned embryo in recipient and improving the efficiency of cloning.

本试验以波尔山羊耳成纤维细胞为供体，分别以牛、羊、兔的卵母细胞为受体进行体细胞核移植，构建了山羊-兔、山羊-牛和山羊-山羊三种重构胚。研究了不同的胞质环境对重构胚体外发育的影响与核移植中的胞质效应，并对异种克隆胚胎采用了去透明带移植和配种后再移植的方法，探讨移植方法和妊娠因子对异种克隆胚胎在受体动物体内发育的影响，旨在为解决异种克隆胚移植不易着床发育的难题和为提高动物克隆效率提供理论依据。

The experiment results were as following:All cytoplast recipients derived from oocytes of rabbit, bovine and goat can support early development of cloned embryo, the fusion rate of reconstructed embryos of goat-bovine and goat-rabbit were 59.91% and 74.10%, their cleaved rate were 57.48% and 72.57%, blastocyst rate were 9.59% and18.06% respectively, and the fusion of reconstructed embryos of goat-rabbit and goat-goat were 60.01% and 74.10%, their cleaved rate were 56.68% and 72.57%, blastocyst rate were 11.76% and18.06% respectively.

试验获得了如下结果：1。以山羊耳成纤维细胞为供体，以牛、羊、兔卵母细胞做为胞质受体构建重构胚，并经体外培养，发现不同的胞质受体均能支持重构胚的早期发育，但受体胞质对重构卵的重构胚早期发育速度和卵裂率均有显著影响。

Dam of top class winners up and down the country.

她是整个国家上下很多顶级获奖鸽的母辈，祖母辈和曾祖母辈。

When Stalingrad fell in January 1943, after 300,000 German soldiers had been killed, Goebbels banned the song entirely, saying that "a dance of death roamed throughout its bard."

安德森录制这首歌所用的母带。1943年1月，当在斯大林格勒会战中被打败、30万德军阵亡之后，戈培尔下令彻底禁播这首歌，说它是"弥漫在酒吧中的死亡舞曲"。

He ordered the original master copy of Lale Andersen's recording destroyed. When Stalingrad fell in January 1943, after 300,000 German soldiers had been killed, Goebbels banned the song entirely, saying that a dance of death roamed throughout its bard.

他命令销毁拉尔·安德森录制这首歌所用的母带。1943年1月，当在斯大林格勒会战中被打败、30万德军阵亡之后，戈培尔下令彻底禁播这首歌，说它是"弥漫在酒吧中的死亡舞曲"。

He ordered the original master copy of Lale http://sem.qsnook.com Andersen's recording destroyed. When Stalingrad fell in January 1943, after 300,000 German soldiers had been killed, Goebbels banned the song entirely, saying that "a dance of death roamed throughout its bard."

他命令销毁拉尔·安德森录制这首歌所用的母带。1943年1月，当在斯大林格勒会战中被打败、30万德军阵亡之后，戈培尔下令彻底禁播这首歌，说它是"弥漫在酒吧中的死亡舞曲"。

Dardanus had a son, king Erichthonius, who was wealthiest of all men living; he had three thousand mares that fed by the water-meadows, they and their foals with them.

以后，达耳达诺斯生养一子，王者厄里克索尼俄斯，世间最富有的凡人，拥有三千匹母马，牧养在多草的泽地，盛年的骒马，高傲地看育着活蹦乱跳的仔驹。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。