母

The results showed that the rates of mature oocyte were 75.2％, 73.1％, 69.8％, 63.5％and oocyte at telophase of MI were 16.3％, 15.9％, 16.9％, 27.0％, respectively. The rates of maturation of oocytes cultured in TCM199 with serum, EGF and TGFαwere significantly higher than that of with BSA team (P＜0.05) and the number of oocyte stayed at telophase of MI in TCM199 with serum, EGF and TGFαteam were significantly less than that with BSA team(P＜0.05); Significant higher rates (66.6％, 66.6％, 73.6％) of normalα-tubulin distribution in oocytes cultured in TCM199+ serum, EGF and TGFαwere compared to that of TCM199+BSA(43.3％)(P＜0.05). The rates of oocyte with cortical granules in cortex were 58.8％, 33.9％, 54.7％and 47.9％respectively, there was significant difference between oocytes cultured with serum, EGF and BSA(P＜0.05). In conclusion, TGFαand EGF can promote the oocyte nuclear transition from telophaseⅠto metaphase of meiosisⅡ, and improved the expression and distribution ofα-tubulin during the ovine oocytes maturation; EGF and serum could promote oocyte cytoplasm maturation. The results suggested that EGF and TGFαmight substitute some substance in serum to improve the quality of oocyte nucleus maturation in vitro, but EGF might be more functional than TGFαto promote the maturation of ovine oocyte ooplasm.

三、EGF和TGFα对卵母细胞成熟的影响未成熟卵母细胞分别在TCM199基础培养液+血清、TCM199基础培养液+BSA+EGF、TCM199基础培养液+BSA+TGFα和TCM199基础培养液+BSA四组成熟培养系统中成熟培养，22小时后上述各组卵母细胞的核成熟率分别为75.2％、73.1％、69.8％、63.5％，处于第一次减数分裂末期的比率分别为16.3％、15.9％、16.9％、27.0％，在添加血清、EGF、TGFα各组中核成熟率显著高于其它各组(P＜0.05)，处于末期的比例明显低于其它各组(P＜0.05)：添加血清、EGF、TGFα各组在a-Tubulin蛋白的分布与表达上(66.6％、66.6％、73.6％)明显高于BSA组的正常率43.3％(P＜0.05)；各组CG发生迁移较好的卵母细胞的比率分别为58.8％、33.9％、54.7％、47.9％，血清组和添加EGF组CG迁移至皮质区的比例明显高于仅添加BSA处理组(P＜0.05)。

The result of frozen MⅡoocytes using three methods indcated that the self-restraintspear method was better than OPS, and the effection of frozen altogether with self-restraintspear method and centrifugal was the best; the frozen effection was not ideal using fluidspear for 8- cell ovocyte.

用三种冷冻方法(OPS法；自制移液枪头法；移液枪头法+离心法)对MⅡ卵母细胞冷冻的结果表明，自制移液枪头法优于OPS法，其中以移液枪头法+离心组的冷冻效果最好；用移液枪头法对体外发育到8-细胞期卵母细胞的冷冻效果不理想。MⅡ期卵母细胞用OPS法冷冻后卵母细胞IVF和电激活的卵裂率都显著高于移液枪头法(P＜0.01)，电激活后2-细胞卵裂率比IVF高。

The samples of single building up are made of the same we lding procedure in DT3, 20, 45, T8 style steel. The result shows, the bond of lo w carbon base-material consists of smooth white light zone and needle-like l ayer of Martensite, the bond of medium and high base-material consists of sawt ooth structure and unmixed zone, and in medium c...

实验结果表明：低碳钢母材接头熔合区由较平整的白亮带和针状类马氏体层组成；中、高碳钢母材接头熔合区由&锯齿形&组织和不完全混合区组成，其中，中碳钢母材接头的不完全混合区组织为类马氏体，高碳钢母材接头不完全混合区类马氏体层消失，为奥氏体。

High concentration white masterbatch is prepared by using different titanium dioxide, PE wax and adding nanocalcium carbonate etc. to change the formulation of masterbatch in this paper. Hiding power, tinctorial power and dispersion performance were tested by color measure instrument and IBAS, and process performance, rheology were tested by Brabender torsion rheometer and INSTRON rheometer.

本文采用不同的钛白粉、聚乙烯蜡及添加纳米碳酸钙等助剂的方法改变浓色母粒的配方，加工制备浓白色母粒，通过测色仪、图像分析仪测试母粒的着色力、遮盖力和分散性能，通过转矩流变仪和INSTRON流变仪研究了母粒的加工性能和静态流变性能。

The results show that mechanical heterogeneity has great influence on the J-integral of welded joint containing transverse crack. The J-integral on the path cross the interface between welded joint and base metal is higher than the J-integral on the paths in the weld metal for undermatched joint,while the J-integral on the path cross the interface between weld metal and base metal is lower than the J-integral on the paths in the weld metal for overmatched joint. Moreover,the difference of J-integral values increases with the increasing of the load and strength mismatching difference and the path-enclosed length of interface between weld metal and base metal.

计算结果表明：力学不均匀性对含横向裂纹焊接接头的J积分有重要影响，低匹配焊接接头中积分路径穿越焊缝与母材界面的J积分值高于积分路径在焊缝内的J积分值，高匹配焊接接头中积分路径穿越焊缝与母材界面的J积分值低于积分路径在焊缝内的J积分值；载荷越大，焊缝与母材的强度差别越大；积分路径中包含的焊缝与母材界面长度越大，J积分的差值越大。

Under the transmission election microscope, we had observed that the state of oocytes arid the cumulus cells around them was tightness, and there were plenty of long and thin microvilli playing the role of connection in the enwrapping. The shape of cumulus cells nucleus was ovate, and the longer and shorter diameters of the cumulus cells were 4.250±0.042μm and 2.750±0.07 μm respectively. The zona pellucida was well proportioned and tightly linking with oocyte. There were great deals of multi-crista mitochondria, lipid droplet and vacuolus. The mitochondria longer and shorter diameters were 0.600±0.106μm and 0.490±0.117μm. Granular nucleoplasma distributed very well in the cell nucleus.

电镜下，卵母细胞外的卵丘细胞紧紧包裹卵母细胞，且与卵母细胞之间有大量细长的微绒毛相联系；卵丘细胞核呈卵圆形，其长径为4250±0.042μm，短径为2.750±0.071μm；透明带均匀，与卵母细胞结合紧密；在皮质区集中分布大量的多峰型线粗体、脂滴和空泡，线拉体长径为0.600±0.106μm，短径为0.490±0.117μm；细胞核中颗粒状的核质分布非常均匀。

Stage I of both male and female stone flounder appears only once in all its life, and the germ cells are comprised by spermatogonia or oogonia. The gonad from Mar to Aug keeps at stage II. The gonad index of testis at this stage is 0.037%, and the amount of spermatogonia is increased quickly. There is some linear germ plasm in the cytoplasm of spermatogina. In ovary at this stage it is mostly composed by oocyte of phase 2 which the character is the appearance of yolk nucleus, and no zona radiate in membrane. The mean GI of ovary is 1.95%. From Sep to Oct gonad is at stage III which testis is composed by lots of spermatogina and few spermatocytes, and the mean GI of testis at this stage is 0.086%. In ovary the ooctyes at phase 3 are in dominate position, the yolk nucleus disappear. And the GI of this stage is 3.35%. Both testis and ovary are at stage IV in Nov. hi testis the germ cells are in spermiogenesis, and the mean GI is 0.93%. hi ovary the oocytes are mostly at phase 4, which are filled in the cytoplasm with vitellin granule, and the zona radiate in membrane begins to formation. Nucleus moves to one side of the oocyte gradually. The mean GI of ovary at this stage is 9.37%.

在每年的3月-8月期间性腺处于Ⅱ期，此期精巢中精原细胞明显增多，胞质局部可见有线状的生殖质存在，平均成熟系数为0.037％；卵巢中以2时相卵母细胞为主，可见细胞质中出现强嗜碱性的卵黄核，细胞外由一层滤泡细胞包围，但尚无放射带，平均成熟系数为1.95％。9月-10月期间性腺处于Ⅲ期，此期精巢中仍有大量精原细胞，同时可见部分精母细胞，平均成熟系数为0.086％；卵巢中以3时石鲜孟加限加$玩印面n洲匆s性腺发生、分化及发育的周年变化相卵母细胞为主，细胞质中的卵黄核己消失，平均成熟系数为3.35%。11月性腺处于IV期，此期精巢内精细胞正处于不同的形成过程中，平均成熟系数为0.93%；卵巢中以4时相卵母细胞为主，胞质中充满染成桔红色的卵黄颗粒。

The Company was founded in the 1994,Main exploituree and product currency plastic masterbatch,engineering masterbatch,flame retardant masterbatch,function masterbatch,automobile material and high-tech product,adopt computer match colors,and privides matches the pigment service,dyeing pelleting service.

公司成立于1994年，主要研发及生产通用塑料母料、工程母料、阻燃母料、功能母料、汽车料等高科技产品，采用电脑配色，并提供配色粉服务，来料染色造粒服务。

This is a crucial stage in the normal psycho-sexual developmental process, and those who fail to pass the stage will be unable to establish a normal man-woman relationship when they grow up. After introducing the origin and understanding of Mother Fixation, the thesis points out that D. H. Lawrence, together with his works, especially Sons and Lovers, is a specific evidence of the Oedipus complex. In the traditional Chinese family culture,combining with some of the special relationship of the sons and mothers and the conflicts between the mothers and daughters-in-law they reveals the phenomenon and essence of Mother Fixation.

本文旨在细述对恋母情结的来源和对恋母情结的理解后，指出20世纪英国作家劳伦斯及其作品，尤其是《儿子与情人》，便是&恋母情结&的有力佐证，并从劳伦斯的生平和这部作品中找出事实与证据，结合中国传统家庭文化中某些特殊的母子关系、婆媳的纷争与矛盾关系中揭示了恋母情结的现象和本质。

This is a crucial stage in the normal psycho-sexual developmental process, and those who fail to pass the stage will be unable to establish a normal man-woman relationship when they grow up. After introducing the origin and understanding of Mother Fixation , the thesis points out that D. H. Law rence, together with his works, especially Sons and Lovers, is a specific evidence of the Oedipus complex. In the traditional Chinese family culture,combining with some of the special relationship of the sons and mothers and the conflicts between the mothers and daughters-in-law they reveals the phenomenon and essence of Mother Fixation.

本文旨在细述对恋母情结的来源和对恋母情结的理解后，指出20世纪英国作家劳伦斯及其作品，尤其是《儿子与情人》，便是&恋母情结&的有力佐证，并从劳伦斯的生平和这部作品中找出事实与证据，结合中国传统家庭文化中某些特殊的母子关系、婆媳的纷争与矛盾关系中揭示了恋母情结的现象和本质。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐