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It is concluded that, first, geochemical data of the surface soils can clearly distinguish two kind of soils, that is, juvenile soils, for example, purple soils, which inherits most geochemical characteristics of the parent rocks or materials, and anthropic soils, the geochemical characteristics of which have been greatly changed as the results of intensive impact from variety of agricultural uses. Second, different soils have typical indicator elements association respectively due to different parent rocks or materials and utilization, such as purple soils, yellow soils (low alkali and alkaline-earth metal contents and pH value), paddy soils (high organic element and heavy metal elements contents) and fluvo-aquic soils (high rock-forming accessory mineral elements contents) and so on. Consequently, it can be suggested that elements association is an important indicator for two key factors (parent materials and anthropic activities) of soil classification. Third, some differences exist between soil genesic classification and result of cluster analysis, because geochemical characteristics of surface soils can not completely represent the foundation applying to soil genesic classification, that is, condition and process of soil-forming, and soil property.

结果表明：浅层土壤的地球化学数据能较好地用来区分两类土壤，即紫色土一类的幼年土壤（在很大程度上继承了母岩、母质的地球化学特征）和受强烈人为活动水耕、早耕熟化的土壤（外来物质的不断加人显著地改变了浅层土壤原有的地球化学性质）；(2)各类土壤因母岩母质和利用方式不同，各具明显的标志性元素组合特征，如紫色土、黄壤（低碱和碱土金属元素和pH值）、水稻土（高有机元素、肥料元素和重金属元素）和潮土等，因此，这些元素组合是土壤分类两个要素的重要参照系；(3)浅层土壤的地球化学特征不可能完整地体现成土条件、成土过程和土壤属性这三个发生分类依据，因此，聚类分析结果与发生类型之间存在一定的差异。

The experiment is designed to determine the optimum mating time of the bitches by studying the changes of the corneous cells in the vagina of the naturally estrous bitches.

而由于良种母犬的配种费用较高，加上犬主人对提高良种母犬繁殖率的期望值较大，特别希望能帮助确定母犬的准确配种时间，以收到更好的繁殖效果，因此，有必要研究母犬排卵时间的鉴定方法。

Techniques in embryo technology (such as in vitro production of embryos and animal cloning) need large quantities of high quality oocytes. But the quality of in vitro matured oocytes from slaughtered animals is generally lower than that of the in vivo matured oocytes. It is usually thought that the reason for this poor quality in in vitro matured oocytes is the lack of capacitation during the dominancy of follicular development in vivo.

目前胚胎工程技术研究和开发(如体外生产胚胎和体细胞克隆等)需要大量高质量的成熟卵母细胞，但利用屠宰动物卵巢卵母细胞经过体外成熟培养而获取的卵母细胞质量还远不如体内成熟卵母细胞，其原因一般认为是由于缺乏体内主卵泡阶段的获能作用。

The result also showed that return treatment is better than taken treatment within different treatments. 4. According to the experiment when the plant was harvested(In March 25th,2009), it was found that the rape with NPK treatment,especially with NPK-T treatment in Quaternary red soil and in granitization red soil, grew abnormality or dead in the centre of district(80%),which yield was extremely low,but had some to the remaining(20%) close to the cement ridge.Among the three types of parent material,eighteen districts,Dischaemum Schreb of gramineae plants could only grow in granitization red soil with NPK treatment,and the other sixteen districts were little or even not.The rape planted in Quaternary red soil with NPK treatment got disease especially in the centre of the district.

其次是M处理，CK最低。3、三种母质种植油菜均表现为：归还处理好于带走处理；4、据试验收产时（2009年3月25日）发现：四纪红土和花岗岩二种母质的NPK处理的油菜除靠近水泥梗边行长有部分油菜外（占小区面积20%），小区中间（占小区面积80%）的油菜生长不正常或死亡，产量极低；三种母质共18个小区，只有花岗岩母质的NPK处理长有禾本科植物马唐草，其余16个小区很少或没有；四纪红土NPK处理油菜病害严重。

Calcium sulfate has been used for bone regenation in oral medicine. Osteopontin, a marker molecule, expressed by osteoblast, plays multiple roles in human body. However, the effect of calcium sulfate on osteopontin expressed in osteoblasts is unclear. In this study, U-2 osteosarcoma osteoblastic cells were cultured in 0, 0.5, 1, or 10 μM calcium sulfate hemihydrate. There was no effect in the proliferation of cells, suggesting that calcium sulfate up to 10 μM is biocompatible. The cells and conditioned media were collected for analyzing mRNA and protein levels of osteopontin by reverse-transcription polymerase chain reaction and Western blot, respectively.

硫酸钙用於口腔医学中的骨头再生已经有多年历史，骨桥素是一种被骨母细胞分泌且在人体扮演重要多重角色的标的分子，然而，硫酸钙在骨母细胞分泌硫酸钙上的影响仍未明朗，在本实验之中，培养U-2骨肉瘤类骨母细胞(U-2 osteosarcoma osteoblastic cells)於0、0.5、1、10 μM的硫酸钙之中，在细胞数目的复制上，四种硫酸钙浓度并无影响，显示出U-2骨肉瘤类骨母细胞培养於10 μM的硫酸钙仍是生物相容性的。

They took the four wolf cubs to the wolf for milk and gave the wolf some food of them in order to make them survived. Also, they camped there to protect the family for the indefensibility of the wolf.

他把自己的食物分给母狼吃，以维持母狼的生命，晚上还得在母狼附近露营，保护这个狼家庭，因为母狼被夹住了，无法自卫。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

Pig oocytes cultured in vitro for some time were inseminated by frozen–thawed ejaculated sperm. At specified times after insemination, sperm penetration, cell cycle progression and mitogen-activated protein kinase phosphorylation were evaluated. It was shown that:(1) oocytes at various maturational stages could be penetrated by sperm;(2) sperm penetration did not affect meiotic cell cycle progression;(3) sperm penetration of germinal vesicle oocytes and maturing oocytes did not alter MAPK phosphorylation; and (4) when premetaphase I and metaphase I oocytes, in which MAPK was activated, were fertilised, no evident MAPK dephosphorylation was detected as in metaphase II oocytes.

不同成熟阶段的猪卵与精子融合后体外培养发现：(1)不同成熟阶段的猪卵母细胞都可被精子穿透；(2)精子穿透不影响减数分裂细胞周期进程；(3)精子穿透GV期卵母细胞和正在成熟的卵母细胞不改变MAPK磷酸化：(4)已受精的前中期I和中期I卵母细胞的MAPK有活性，MAPK不像MII卵母细胞那样受精后发生磷酸化。

However, when two maturing oocytes fused and two spindle will form in the big cell, the chromosomes will not intermingle. In the present study, we removed GV from one oocyte and transfer to the perivetelline space of another GV oocyte. After fusion the resulting oocytes which contained two GVs were cultured further in MEM.

在本实验中，我们利用小鼠GV期卵母细胞将一枚卵母细胞的生发泡取出后移至另一未经去核处理的GV期卵母细胞透明带下，经三次电脉冲作用后将融合的含有两个GV的卵母细胞放入MEM成熟培养液中培养，在培养成熟的不同阶段分别收集卵母细胞进行免疫荧光染色观察微管及核的变化。

The results showed that after 2 h of culture in MEM the chromosomes of oocyte were seperated to form telophase I while a small spindle was observed around chromosomes of primary spermatocyte. However, two clear spindles were observed in the oocytes cultured in CB containing MEM. After further culture, the chromosomes of both primary spermatocyte and oocyte intermingled and formed one large spindle.

在无CB的培养液中培养的卵母细胞培养2小时后，卵母细胞已经进入第一次减数分裂的后期，染色体开始被拉向两极，而精母细胞的MI纺锤体才刚刚形成，虽然继续培养两者染色体可以合二为一并形成一个纺锤体，但是有些染色体发生滞后；当卵母细胞在含有CB的培养液中培养2小时后，在卵母细胞内形成两个相似大小的MI纺锤体，进一步培养形成一个大的纺锤体，染色体正常。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐