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The results showed that 1 there were no significant differences in the rates of cytoplast protrusion and enucleation between oocytes that were incubated in colchicine (0.4 μg/mL) for 0.5 h and oocytes that were incubated in colchicine (0.4 μg/mL) for 1 h, and the rate of cytoplast protusion can be 85.4% while the rate of cytoplast enucleation is 100%. 2 There was no significant difference in oocyte enucleation between oocytes treated with medium containing 0.2 μg/mL colchicine for 0.5 h and oocytes treated with medium containing 0.4 μg/mL colchicine for 0.5 h. 3 A maturation time of 18–23 h did not affect the rates of cytoplast protusion and enucleation by chemically assisted enucleation, whereas the rate of enucleation of oocytes by blind enucleation was found to decrease with a prolonged incubation time. 4 The development rates of reconstructed embryos could not be influenced by these two enucleation methods, increased from oocytes matured for 21–23 h.

结果表明: 1 卵细胞在0.4 mg/mL的秋水仙素溶液中分别孵育0.5 h和1 h,胞质突起率和去核率没有显著的差异,突起率可高达85.4%,去核率达到100%; 2 0.2 mg/mL或0.4 mg/mL秋水仙素溶液将卵细胞处理0.5 h,对去核效果没有显著影响; 3 对于体外成熟18~23 h的卵细胞,随着成熟时间的延长,盲吸法的去核率降低,但没有影响秋水仙素诱导胞质突起的比率和去核率; 4 两种去核方法对重构胚的发育没有产生显著影响,但成熟21~23 h卵细胞重构胚囊胚的发育率显著高于成熟18~20 h卵细胞重构胚囊胚的发育率。

The oogenesis of P. esculenta can be divided into the following phases in morphological characteristics of oogonium and oocyte development: the proliferative phase of oogonium (0μm in diameter), the initial growth phase of oocyte (10—20μm in diameter), the grand growth phase-Ⅰof oocyte (20—60μm in diameter), the grand growth phase-II of oocyte (60μm×70μm to 120μm×145μm in size), the mature phase of oocyte, and the declining phase, showing a dynamic changes in oogenesis of P. esculenta .

以卵原细胞与卵细胞发育的形态学特征为依据,将可口革囊星虫的卵子发生过程划分为:卵原细胞增殖期(卵径0μm)、卵细胞小生长期(卵径10—20μm)、卵细胞大生长期Ⅰ(卵径20—60μm)、卵细胞大生长期Ⅱ[大小为(60μm×70μm)—(120μm×145μm)]、卵细胞成熟期及退化期6 个阶段,反映了可口革囊星虫卵子发生过程的动态变化。

Different strains have different reaction to the delay of oviducts in body after animal death. After 10min delay C57BL/6 mouse oocytes have death rate of 56.5%,significantely higher than Kunming mouse (47.6%);Spontaneous activation rates were respectively 13.3% and 46.0%, C57BL/6 were obviously lower than Kunming mouse.4. The oviducts were obtained after being delayed 5min 24h after the mice were injected with hCG. The oocytes were cultured in CZB. About 81.1% occurred spontaneous activation, evidently lower than parthenogenetic rate (96.4%) with SrCl_2. Spontaneous activable oocytes had high cleavage rate(93.2%) and 4-cell rate(87.3%). However, spontaneous activable oocytes had blastula development rate(18.7%) as low as parthenogenetic oocytes by SrCl_2(22.9%).

不同品系小鼠卵细胞对输卵管在体内滞留产生的反应不同,滞留10min C57BL/6系小鼠卵细胞死亡率为56.5%,显著高于昆明鼠(47.6%);自发激活率分别为13.3%和46.0%,C57BL/6系小鼠显著低于昆明鼠。4.hCG后24h体内滞留5min卵细胞在CZB中培养自发激活率为81.1%,显著低于SrCl_2孤雌激活率(96.4%);自发激活的卵细胞有较高的卵裂率(93.2%)和4-cell比率(87.3%),但囊胚率(18.7%)较低,同卵龄的卵细胞经SrCl_2孤雌激活囊胚发育率为22.9%,差异不显著。

Beginning in 2004 the company entered the plastics masterbatch industry, after the rapid development has been the formation of masterbatch, filler masterbatch, moistureproof Masterbatch, Masterbatch four series features dozens of varieties.

公司从2004年开始进入塑料料行业,经过快速发展,现已形成色料、填充料、防潮料、功能料四大系列几十个品种。

In conclusion, these results suggested that (1) the ageing-associated decline in fertility of female KM mouse was due to a decrease in both quantity and quality of oocytes;(2) the ooplasm from young mice did not correct the meiotic errors of aged mice, and the ooplasm from aged mice did not induce abnormal segregation of meiotic chromosome of young mice, indicating that meiotic anomalies found in the oocytes of aged mice might be related to nucleus or chromosomes and relevant factors;(3) a critical nucleocytoplasmic ratio was essential for normal maturation and segregation of meiotic chromosomes of oocytes and development to 2-cell embryos.

本研究结果表明:(1)昆明白小鼠与衰老相关的生育力下降是其卵细胞数量减少和质量下降的综合结果;(2)年轻小鼠卵细胞细胞质不能纠正老龄小鼠GV的减数分裂错误,老龄小鼠卵细胞细胞质也不诱导年轻小鼠的GV发生减数分裂错误,老龄小鼠卵细胞减数分裂异常很可能与细胞核或染色体及其相关因素有关;(3)关键的核质比对卵细胞正常的成熟、减数分裂染色体分离及2-细胞期胚的发育是绝对必需的。

To pronuclear formation rate after oocyte in vitro maturation 18 hours and parthenogenticactivation, oocytes derived from SM is significantly higher than oocytes from PM,oocytes derived from IM is significantly lower that of oocytes detrived from PM and SM.

对于孤雌刺激反应而言,无论是何种类型的卵细胞成熟18小时后,自发成熟卵细胞激活后形成原核的比率高于被动成熟的卵细胞,诱导成熟的卵细胞的原核形成率显著低于自发和/或被动成熟的卵细胞。

The testis index, testis volumes were same as the annual changes of testis mass. The curves of annual variation were all unimodality.2 The spermatogenetic cycle of Myospalax cansus comprises seven stages with significant features: Stage I , from February to April, the testis were at the stage of spermatogonia proliferation. In this period, testis index and the number of spermatogonia began to rise. Other spermatogenic cells had not yet formed; Stage II to III, from March to April, primary spermatocyte meiosis period. The testis index was highest in this stage, and spermatogenic cells were in spermatocyte stage, the primary spermatocyte meiosis generated to secondary spermatocyte; Stage IV, from April to May, spermatocytes continued to split, germ cells appeared in seminiferous tubules; Stage V, in May, sperm formation, spermatids of seminiferous tubules were transformed to spermatozoa, a large number of sperms existed in the lumen; Stage VI, spermatozoa emission period, from May to June, testis index were a significant drop and mature spermatozoa excluded gradually; VII, the testicular activity ceased basically from July to September, November to January of the following year, the spermatogenic activity ceased completely. Therefore, Myospalax cansus are animals of seasonal reproduction, spermatogenesis cycle is discontinuous type.

睾丸系数、体积和重量的年周期变化规律一致,变化曲线呈现单峰型。2甘肃鼢鼠雄性生殖腺的年周期活动由7个特征明显的时期构成:Ⅰ期,2~3月份,精原细胞增殖期,睾丸系数开始上升,精原细胞进行有丝分裂,其他生精细胞尚未形成;3~4月份为Ⅱ~Ⅲ期,初级精细胞成熟分裂期,睾丸系数达到最大,生精细胞大多处于精细胞阶段,初级精细胞减数分裂生成次级精细胞;Ⅳ期,4~5月份精细胞继续进行分裂,精细胞在生精小管内出现;Ⅴ期,5月份,精子形成期,曲细精管中精细胞变态成精子,在管腔中存在大量的精子;Ⅵ期,精子排放期,5~6月份,睾丸系数显著下降,成熟精子从生精小管上脱离,逐渐排除;Ⅶ期,精原细胞停滞期,7~9月份睾丸生精活动基本停滞,11~翌年1月,生精活动完全停止。

The apoptosis of 80 mg/kg ﹒ d group was mainly located in spermatogenous cell and first spermatocyte; the apoptosis of 120 mg/kg ﹒ d group was mainly located in spermatogenous cell , first spermatocyte and spermatocyte of the second order; the apoptosis of 240 mg/kg ﹒ d group was mainly located in spermatogenous cell and first spermatocytes, and seemingly, the apoptosis located in first spermatocyte was dominant; whereas the apoptosis of 480 mg/kg ﹒ d group was located in all levels spermatogenic cells.

结果表明:各试验组小鼠生精细胞凋亡指数随着浓度的升高有增加的趋势,而且与对照组差异显著( P <0.05或 P <0.01)。80 mg/kg · d 组凋亡细胞主要定位于精原细胞和初级精细胞;120 mg/kg · d 组凋亡细胞主要定位于精原细胞、初级精细胞和次级精细胞;240 mg/kg · d 组凋亡细胞主要定位于精原细胞、初级精细胞,以初级精细胞为最多;而480 mg/kg · d 组凋亡细胞定位于各级生精细胞。

Removal of tannin by washing grapeseeds with alcohol resulted in oil with low pigment content (K410 and K670 values).

葡萄种子的热处理,提高了葡萄种子油中主要维生素E单体γ-育三烯酚和α-育酚、β-育酚、γ-育酚的含量,降低了δ-育酚的含量,葡萄种子去单宁后大大降低了生育酚的含量。

I added the White Tara at the center, and arranged the remaining Taras in their usual sequential order as taught by Guru Chen in his works clockwise around the central Green and White Taras. So it is a Tara Mandala.

我在中间加入了白度,并且将其他的度依陈上师在他教授中所示的顺序以顺时针方向,环绕中间的绿度及白度,因此这是一个度坛城。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。