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Hormone level in maturation medium affected cumulus expansion of COC, but cumulus expansion became less dependent on hormone level with time of culture. Porcine immatured oocytes, granulosa cells from small antral follicles and cumulus cells of immatured oocytes secreted cumulus-expansion enabling factors. Granulosa cells from 3-6mm follicles did not produce CEEF, but there was some CEEF in the follicular fluid of this sized follicles.

激素水平对卵丘扩展有影响，但随培养时间的延长，对激素的要求有逐步减少的趋势；猪卵丘扩展不依赖于卵母细胞，卵母细胞核成熟也与卵丘扩展无关；GV期卵母细胞、小腔卵泡内颗粒细胞和成熟卵的卵丘细胞都有较高的分泌CEEF能力；3-6mm卵泡的壁颗粒细胞不能分泌CEEF，但3—6mm卵泡的卵泡液中有CEEF。

Hormone level in maturation medium affected cumulus expansion of COC,but cumulusexpansion became less dependent on hormone level with time of culture.Porcine immaturedoocytes,granulosa cells from small antral follicles and cumulus cells of immatured oocytessecreted cumulus-expansion enabling factors.Granulosa cells from 3-6mm follicles did notproduce CEEF,but there was some CEEF in the follicular fluid of this sized follicles.Sun xingshen, Animal Histology and Embryology

激素水平对卵丘扩展有影响，但随培养时间的延长，对激素的要求有逐步减少的趋势；猪卵丘扩展不依赖于卵母细胞，卵母细胞核成熟也与卵丘扩展无关；GV期卵母细胞、小腔卵泡内颗粒细胞和成熟卵的卵丘细胞都有较高的分泌CEEF能力；3-6mm卵泡的壁颗粒细胞不能分泌CEEF，但3-6mm卵泡的卵泡液中有CEEF。

Myoid/myofibroblastic areas in DFSP possibly represents the hyperplasia of stromal myofibroblasts, rather than true myofibroblastic differentiation of the neoplastic cells.

DFSP中的肌样／肌纤维母细胞性分化可能是肿瘤间质中肌纤维母细胞增生的结果，并非代表了瘤细胞的真性肌纤维母细胞性分化。

After the oestrus season, mother and her offspring also hold some social relations and interactions.

发情季节后，从母兽产仔到幼仔离开母兽，母仔之间存在一定的社会关系和相互作用。

About 48%(90/194) were below 29 years of age. Ninety-nine of 194 cases had either radiotherapy alone or supplementary treatment with radiotherapy following surgery. We analyzed multiple prognostic factors. The factors of importance included postoperative radiotherapy; female in glioblastoma multiforme; the primary tumor dose above 5000 rads and/or whole brain irradiation in malignant astrocytoma and glioblastoma multiforme; the primary tumor dose above 4500 rads in ependymoma, medulloblastoma and oligodendroglioma; and spinal axis irradiation above 2000 rads in ependymoma and medulloblastoma that produced a better prognosis than in other groups.

男与女之比例为2.7比1，年龄分布自1至81岁，平均年龄33.3±19.3岁，中值年龄为34岁，29岁以下占48%(90/194)，其中99例单独或手术后辅以放射腺治疗，分析同种脑瘤的预后因素，其中多形神经胶母细胞瘤有手术后放射线治疗或女性，多形神经胶母细胞瘤合并星细胞瘤原发肿瘤剂量5000雷得以上或曾全颅腔照射，神经管胚细胞瘤，间胶质母细胞瘤，及室管膜瘤原发肿瘤剂量4500雷得以上，室管膜瘤及神经管胚细胞瘤有脊轴照射2000雷得以上预后均佳，且统计上有显著的差异。

To eplore the relationship between Dynein and the formation of meiosis error of oocytes, the immature mouse oocytes were in vitro cultured with a known dynein ATPase activity inhibitor- sodium orthovanadate to detect the changes of maturation rate, and semi-quantitative RT-PCR and single cell RT-PCR were performed to detect the alteration of the cyclin B1 mRNA level.

为探讨Dynein与卵母细胞减数分裂错误形成的关系，采用小鼠卵母细胞体外培养成熟技术和半定量RT-PCR方法，分别检测Dynein抑制剂-原钒酸钠对卵母细胞成熟率和cyclin B1基因转录水平的影响。

Objective:To study the diagnostic and differential criteria for benign and malignant osteoblastoma.

骨母细胞瘤临床病理学研究李亦民丁彦青徐莉摘要探讨良性骨母细胞瘤和恶性骨母细胞瘤的诊断和鉴别诊断标准。

Methods: Immunohistochemical study of CD133 expression: 55 cases osteosarcoma conventional paraffin-embedded tissues specimens come from Pathology Department Qilu Hospital Shandong University, including 4 cases parosteal osteosarcoma, 13 cases fibroblast-type osteosarcoma , 12 cases chondroblastic osteosarcoma and 26 cases Osteoblastoma osteosarcoma.

免疫组化研究CD133的表达：取自本院病理科的骨肉瘤常规石蜡包埋组织蜡块标本55例，其中骨旁骨肉瘤4例，纤维母细胞型骨肉瘤13例，软骨母细胞型骨肉瘤12例，骨母细胞型骨肉瘤26例，切成3μm的组织切片备用。

When Fas and c-myc protein were coexpressed in cytoplasm, a strong positive sign appeared in highly differentiated osteosarcoma, fibrous dysplasia of bone and osteoplast of bone callus, a weak sign was found in low-differentiated tumour cells and fibroblasts.

Fas和c-myc蛋白胞浆内阳性，在骨肉瘤的高分化细胞(骨母细胞型和软骨细胞型瘤细胞)和骨化性纤维瘤、骨纤维结构不良、骨痂组织中骨母细胞呈强表达；而在低分化瘤细胞和纤维母细胞则呈弱表达。

This experiment first studied in the mature nutrient medium to increase the hormone to the porine ovocyte in vitro mature process in the chromosome configuration and the mature rate influence, by explored the porine ovocyte in vitro mature rule, enhanced in vitro raise maturity, was the ROSI technology system preparation high quality ovocyte; Then has carried on the experiment in view of the porine round sperm cell in vitro separation, through to the ROSI operation and ICSI (Intracytoplasmic sperm injection, the comparison which ICSI) operated studies has affected the ROSI operation the factor.

本试验首先研究了成熟培养液中添加激素对猪卵母细胞体外成熟过程中染色体构型和成熟率的影响，以探索猪卵母细胞体外成熟规律，提高体外培养成熟度，为ROSI技术体系准备优质卵母细胞；然后针对猪圆形精细胞的体外分离进行了试验，进而通过对ROSI操作和ICSI(Intracytoplasmic sperm injection，ICSI)操作的比较研究了影响ROSI操作的因素。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐