残液

Thermally denatured DNA was preconcentrated at pretreated GCE by its adsorption in open circuit for 5min or at the potential of+0.3V for 90s, and produced two well-defined oxidation peaks of guanine and adenine residues at+0.80V and+1. 11V in pH 5. 0 phospoate buffer, respectively, but the native DNA did not produce any peaks at the same conditions.

在pH 5.0的磷酸盐缓冲液中，于+0.3V富集90秒或在开路条件下吸附富集5分钟，热变性DNA可以吸附富集在预处理玻碳电极表面，阳极扫描时，鸟嘌呤和腺嘌呤残基产生两个很好的氧化峰，峰电位分别为+0.80V和+1.11V，而在同样条件下，天然DNA却几乎不出现峰。

By setting the retting duration of 20 hours, the factors affecting the residual gum percentage of the retted kenaf fibers are studied. The experimental results show clearly that the following factors have the significant influence on the residual gum percentage of the retted kenaf fibers and show the obvious variation tendency. The preferred processing parameters are as follows: pH value 7. 0、bath ratio 1: 50、reuse percentage of retting liquid 50%、air flow volume 2. 5L/min、temperature 40℃、nitric additives (ammonia is better than nitrates) percentage 0.45%.By comparison with anaerobic retting, the retting duration using aerobes is much shorter than that using anaerobes.

确定了以好氧微生物脱胶作为本课题的脱胶路线后，在特定的脱胶时间（20小时）条件下，通过分析不同参数条件下的残胶率，研究了好氧微生物脱胶的各种影响因素对红麻脱胶的影响规律，并优选出最佳脱胶液回用比为50％，最佳pH值为7.0，最佳气流量为2.5L/min，最佳浴比为1：50，最佳水浴温度为40℃，最佳含氮添加剂为铵盐类化合物，最佳含氮添加剂用量为0.45％。

The breeding of saccharomycete on resist high alcohol concentration in the fermentational sweet sorghum juice;2. Study on producing fuel ethyl alcohol by sweet sorghum juice;3. As a substitute for food supplies, sweet sorghum juice with high grade has demonstrated outstanding advantage in fermentation.

利用优质甜高粱汁代替粮食进行发酵生产的优越性已经显现，在此基础上研究了面包酵母在甜高粱汁中的生长情况，测定了发酵液中酵母菌的生物量、残总糖等参数，并在10—100l的发酵罐中进行了培养优化实验。

The results of its intrinsic fluorescence spectroscopy and fluorescence phase diagram showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed a partially folded intermediate of Bacillus amyloliquefaciens a-amylase during its unfolding procedure, which followed a three-state model; the result of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed some stable hydrophobic regions, which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid, in the partially folded intermediate of Bacillus amyloliquefaciens a-amylase; and the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed the distribution of Trp residues in Bacillus amyloliquefaciens a-amylase in different denaturation solution, with the maximum number (8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciens a-amylase molecule could be quenched by potassium iodide; and the results of their protein electrophoresis and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciens a-amylase formed during the whole unfolding procedure of Bacillus amyloliquefaciens a-amylase induced by guanidine hydrochloride.

内源荧光光谱和荧光相图结果表明，当变性液中盐酸胍浓度约为1.0 mol/L时，芽孢杆菌a-淀粉酶的去折叠过程中出现一个部分折叠中间体，其去折叠过程符合&三态模型&；荧光探针结果表明，在溶液中盐酸胍浓度约为1.0 mol/L时，中间态芽孢杆菌a-淀粉酶分子中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；荧光猝灭研究给出了不同程度变性的淀粉液化芽孢杆菌a-淀粉酶中的Trp的分布情况，结果表明中间态芽孢杆菌a-淀粉酶分子中能够被碘化钾猝灭的位于分子表面的色氨酸残基数目达到最大的8个；蛋白电泳和体积排阻色谱结果表明，在盐酸胍诱导的芽孢杆菌a-淀粉酶分子的整个去折叠过程中，不会以共价键或非共价键形式形成芽孢杆菌a-淀粉酶分子之间的集聚体或集聚体沉淀。

A pilot-scale experiment for treatment of waste still liquor by using supercritical water oxidation reactors is discussed.

利用超临界水氧化中试装置对盐酸硫胺生产中排放的高浓度残釜液进行处理。

Sterilization in desinfection chamber and subcultures of collections ;②confection of fermentation media and sterilization technics;③influences to objective produce altering the fermentable conditions ,including the examinations of the parameters about remnant sugar、pH、growth of microorganisms and produce;④separations and distillations of objective produces in fermented broth,the distillation technics of filter press and ion exchange;⑤brief and operation techniques of automatic fermentation tank.

课程内容：本课程主要实验内容：①微生物无菌室灭菌及种子扩大培养技术；②微生物发酵培养基及配制及灭菌技术；③微生物发酵及发酵条件的改变对目的产物代谢的影响，包括菌体生长、pH、残糖和产物等发酵过程中各项参数的检测；④发酵液中目的产物的分离及提纯，板框压滤离子交换提取技术；⑤自动发酵罐的简介及操作技术。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。