残株

Four groups of oligonucleotides were successively cloned in pMD18-T vector to obtain four large fragments. Ligation of these four fragments resulted in a cloned DNA MgE717 coding for the 29～268 amino acid region of gE. Sequencing result showed that there is a mutation at 255 amino acid, Asn instead of Asp.

分四段分别合成，基因拼接得到了改造后的基因片段MgE717，经测序，除了在gE基因+763位核苷酸处存在一碱基置换，导致第255位的氨基酸残基由Asp突变为Asn，其余的序列与PrV鄂A株gE基因的氨基酸序列相符。

Based on the biochemical analysis of the two proteins in vitro, the results of yeast two-hybrid experiment and protein-interaction in both wild type cells and C48 strain, we conclude HetR can form a homodimer through the Cys48 residue. We also show that the dimerization is required for heterocyst differentiation.

基于体外两种蛋白的性质比较和酵母双杂交的结果，并结合相应蓝细菌野生型和突变株C48A中HetR蛋白性质的体内研究表明：HetR蛋白可以通过第48位半胱氨酸残基形成共价结合的同源二聚体，该同源二聚体是异型胞正常分化所必需的。

The survival of tobacco mosaic virus in different flue-cured plant residues and other host plants in Guizhou was studied.

研究了贵州烟草普通花叶病毒在烤烟不同烟株残体和其它寄主植物上的越冬存活情况。

In the rice leaves infected by RDV Yunnan isolate, the distribution of cells full of virus particles formed a belt which was a center of infection and multiplication of virus, around the cells fall of virus particles were some cells with the different extent of lesion as follows: disappearance of membrane system of chloroplasts, there were virus particles in some chloroplasts, and some sine or assembled virions in the mitochodria or cytoplasma but not in nuclei ,and intact subcellular structure in the cell far away from the infected center; In the rice leaves cell infected by Zhejiang isolate, some cells were full of virions, but the distribution of cells didn't form the belt, other cytopathological characters were similar to the cells infected by Yunnan isolate.

RDV云南分离株浸染的水稻叶片中，充满病毒粒体的细胞呈条带状分布，呈现出明显的侵染和增殖中心，在充满病毒粒体的细胞周围，相邻细胞有不同程度的病变，叶绿体膜系统消失，部分叶绿体中含有病毒颖粒，线粒体残体及细胞质中有分散或聚集的病毒粒体，细胞核中未见病毒粒体，离侵染中心较远的细胞中亚细胞结构较完整；RDV浙江分离株侵染的水稻叶片中，部分细胞充满病毒粒体，无明显的条带状分布，其他细胞病理特征与RDV云南分离株侵染的水稻叶片相似。

A set of spectific primers was syn thesized according to HBV DNA sequence of Chinese strain, the whole X region was amplified by PCR method from the serum of 9 patients with chronic HBV infection , and then the PCR products were subcloned into pGEM Teasy vectors. Clones were randomly selected to be sequenced. Comparison of the cloned sequence was made to find the difference. After being compared, each sequence of selected clones is o f difference. The point mutation scattered through X region. Deletion mutations were detected in 19 clones of 37(51.4%), which caused different carboxyl endings of X protein. There is a hot region (after 123 aa code) where deletion mutation frequently happens.

以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，自9例慢性HBV感染患者血清中扩增HBV X基因，克隆入pGEM Teasy质粒，随机挑选克隆进行D NA测序以确定病毒的变异程度。37例测序结果提示来源于不同患者HBV X基因序列高度保守，但每个序列均不一致。X区除了存在广泛的碱基点替换突变外，序列的缺失突变占测序克隆总数的51.4％（19／37）；氨基酸缺失及移框突变多发生于123位氨基酸残基之后，可导致X蛋白多种羧基端形式。

Vermeer Corporation of Pella, Iowa, U.S.A., manufactures a full line of grinders, brush chippers, stump grinders and tree spades, as well as horizontal directional drilling systems, mini skid steers, utility and track trenchers and agricultural products.

Vermeer Corporation of Pella， Iowa， U.S.A生产各种磨切机、削片机、残株磨切机和树木铁锹，以及水平定向钻进系统、迷你滑移式装载机、公用设施和轨道挖沟机和农业产品。

All the seed tubers and ratoons in the pots with soil were sterilized after the cutting plants were transplanted.

当植株移植完后，温室中之原薯球、残株及泥土经灭菌处理后销毁。

Not be able to choose the growth of thin and delicate fruit of the plant or after admission rhizome stubble issued pumping big bud, the seedlings grow weak, the results of late, with its high smoking bud propagation of bunchy top disease.

不能选择生长细弱的母株或收果后残株地下茎抽发出的大叶芽，此苗长势弱，结果迟，用其吸芽繁殖容易发生束顶病。

It showed that the yeast KD had higher fermentation capacity, gentle fermentation speed, and the produced kiwi fruit wine was clear and transparent with light yellow color and typical kiwi fruit aroma and wine-alcohol bouquet. Furthermore, the produced kiwi fruit wine had high alcohol content to 10.5 %vol, high vitamin C content to 0.94 g/L and low content of reducing sugar, total acid and volatility acid. 48 aroma substances in total were identified including esters (19 kinds, 34.65 %), higher alcohols(5 kinds,15.38 %), fatty acids(6 kinds,13.30 %), and micro-content of alkyls, aldehydes and ketones, which accounted for 97.33 % of total peak area. Such yeast was suitable for kiwi fruit wine brewing. Tran. by YUE Yang

结果表明，酵母KD的单位酵母发酵力较高，发酵速度平缓，所酿猕猴桃酒澄清、透明，色泽浅黄，酒体醇和爽口，具有猕猴桃果香和纯正的酒香，典型性强；酒度达10.5 %vol，Vc含量为0.94 g/L，残糖、总酸和挥发酸含量均较低；共鉴定出48种香气物质，占总峰面积的97.33 %，其中，酯类物质达19种，相对含量为34.65 %，高级醇(5种，15.38 %)和脂肪酸（6种，13.30 %）含量少，烷烃类和醛酮类等香气物质种类丰富，酒体协调、饱满，是1株适宜猕猴桃酒酿造的优良酵母。

The results showed that, when treated with ground parts degradation products, the height and leaf area of taro plants were increased; the contents of available nitrogen, available potassium, available phosphorus and soil pH value were improved; the yield of taro increased by 15.4%~23.2%, the content of Vitamin C increased by 3.6%, starch content and crude fat content increased by 8.4% and 20.0%, respetively.

结果表明：与对照相比，添加芋艿残体处理芋艿株高和叶面积都有所增加；土壤碱解氮、速效钾、有效磷含量和土壤pH均有不同程度提高；添加芋艿残体可以提高芋艿产量15.4%~23.2%，也可提高芋艿品质，其中维生素C含量提高3.6%，淀粉含量提高8.4%，粗脂肪含量提高20.0%。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。