步骤

Most of these peptides are amidated at their C-terminals, which is essential for their full activities. The a-amide comes from posttranslational modification that is catalyzed by α-amidase (α-AE or PAM). The amidation reaction is a rate-limiting step in the biosynthesis of regulatory peptides.

大部分活性肽的C-端是酰胺化的，而且α-酰胺基为多肽的完全生物活性所必需；α-酰胺基来自多肽的翻译后加工，由α-酰胺化酶催化完成，是酰胺化多肽生物合成途径中的限述步骤。

An amidase from a strain Bacillus cereus ZJB-07112 was purified to homogeneity by using sonication, anion-exchange chromatography, phenyl-sepharose chromatography.

用一株蜡状芽孢杆菌新菌株ZJB-07112 ( Bacillus cereus ZJB-07112)发酵生产酰胺酶，经超声波细胞破碎、High Q阴离子色谱、Phenyl-Sepharose疏水色谱等步骤获得了凝胶电泳均一的酰胺酶。

A amidase from a strain Bacillus cereus ZJB-07112 was purified to homogeneity by using of the sonication, anion-exchange chromatography, Pheny1-Sepharose chromatography.

用一株蜡状芽孢杆菌新菌株ZJB-07112 (Bacillus cereus ZJB-07112)发酵生产酰胺酶，经超声波细胞破碎、High Q阴离子层析、Phenyl-Sepharose疏水层析等步骤获得了凝胶电泳均一的酰胺酶。

Moreover, it was successfully linked with lactobionic acid through amine groups on the surface of particles, and used to recognize living liver cells successfully.(3) In order to simplify the proceeding of preparation of silica-coated qdots, a novel one-pot method using 3-mercaptopropyl-trimethoxysilane as a capping molecule was proposed for the first time. Furthermore, amine and phosphonate groups were also modified on the surface of silica-coated qdots simultaneously. This was the first time the synthesis and the surface modification of qdots were performed in the same system.

3在前一部分工作的基础上，进一步简化量子点的合成与包壳步骤，以MPS为稳定剂，制备了硅壳包被的CdTe量子点，并在硅壳外同步修饰氨基及磷酸基团，首次成功实现了量子点的合成与表面的功能化修饰在同一体系中进行，从而制得了水溶性的、表面带有氨基和磷酸基团的硅壳型CdTe量子点。

AMM formally describes the goal, feature, and indicator of software process, and their relationship. AMM also provides some principles, methods, and techniques to determine the measurement of software process.

模型形式化描述了软件过程的目标、特征和度量指标等关键元素以及相互间的关系，给出了确定软件过程度量的原则、方法和步骤。

The method will involve a sequence of ammonification of glass surface, functionalization of carbon nanotube surface, and bonding between the nanotubes.

将奈米碳管与其它材料以一定的比例复合而制备，进而形成一种具有奈米碳管之复合吸附材料所组成之固相萃取柱介质，其合成步骤包括：奈米碳管表面改质官能化，玻璃粒体之载体氨化及最后将两者经缩合剂作用而产生化学键结使奈米碳管批覆在玻璃粒体之载体上。

The polymethyl methacrylate material with super-hydrophobic surface of the invention is prepared in the following steps: 1 the polymethyl methacrylate material is first put into diamine solution, so that the ester group on the surface of the material has ammonolysis reaction with diamine molecules; 2 and then, the product of the step 1 is put into long chain fatty acid solution to be treated and the solvent is volatilized, so as to obtain the polymethyl methacrylate material with super-hydrophobic surface.

本发明具有超疏水表面的聚甲基丙烯酸甲酯材料，是按照如下步骤进行制备的：1将聚丙烯酸甲酯材料先置于二胺溶液中使聚丙烯酸甲酯材料表面的酯基与二胺分子发生氨解反应；2然后，将其置于长链脂肪酸溶液中进行处理，挥发掉溶剂，得到具有超疏水表面的聚甲基丙烯酸甲酯材料。

If have a friend,be to build a station so, had better be this measure: The first, begin to become good fixed position, search it allow a few keywords to do is good to allow a few keywords to do optimize, for instance, my target is amused this word should accomplish Baidu the first, with etc and the word that concern amusedly, also achieve a goal now, do not believe your Baidu to look.

所以如果有朋友是新建站，最好是这个步骤：第一，开始就做好定位，找准几个关键词做好优化，比如，我的目标就是好玩这个词要做到百度第一，以及其他和好玩有关的词，现在也达到目标了，不信你百度一下看看。

Crystallization was the key step in the process of extraction of amygdalin. Considering the purity and crystallization ratio of amygdalin, the amounts of ethanol and ether was chosen as 1:18 and 1:0.4, crystallization time was 36h.

结晶是分离、纯化苦杏仁苷的主要步骤，本文综合考虑结晶纯度和结晶得率，乙醇添加比为1：18，乙醚添加比为1：0.4 ，结晶时间选择36h。

How to design the software package of control system computer-aided analysis using the graphics user interface development tool of MATLAB are discussed in detail.

简介控制系统的系统分析方法，详细讨论使用MATLAB的图形用户界面开发工具设计控制系统计算机辅助分析软件包步骤和方法，该用户界面具有简单性、一致性、熟悉性，用户能很方便掌握软件包的功能和使用方法。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。