步法

Methods: One-step trizol had used to get the total RNA of the four animal brain, then PEGS000 to purify the small RNA from the total RNA, after the ligation ofpoly on the 3\' and the adaptor on the 5\', the oligo primer will be used to reverse translation the small RNA, and then used the specific primers to amplification the small RNA sequences. After the gel purition recovery, the small RNA will be ligated to the T-vector, and be transformed to the TOP 10 E. coli, at last, we picked up the masc. clones for sequencing.

通过Trizol一步法提取四种动物脑中的总RNA，接着用PEG纯化其中的小分子RNA，再经过poly连尾反应在小RNA的3'端连接上一段poly，然后在5'端连上adaptor，用Oligoprimer将连接好的小RNA进行反转录，用根据adaptor和Oligoprimer设计的引物来扩增小RNA，扩增后的小RNA进行胶回收后连接到T载体上，转化TOP10大肠杆菌后挑取阳性克隆进行测序，得到的序列再经过生物信息学的分析后确定是否为miRNA。

A novel anionic gemini surfactant,didodecyl-ethoxylate bis-sodium sulfate was prepared in three steps using lauryl alcohol,glycol-epiglyceride and chlorosulfonic acid as raw materials and BF_3-ethyl ether as catalyst.The chemical structure of the product was confirmed by IR spectrum.

以十二醇、乙二醇二环氧甘油醚和氯磺酸为原料，以BF3-乙醚为催化剂，通过三步法制备阴离子型双子表面活性剂双十二烷基乙氧基二硫酸酯钠；红外光谱对其结构进行了表征，测定了其表面活性和发泡力等性能。

We tried to find out whether or not the proliferation in dual rearrangements is different from that of correct rearrangement.

并对其中的 117例 NHL采用免疫组化两步法检测 Ki－67蛋白表达。操作按试剂盒说明书进行。

Singles competition for men and women (who are referred to as "ladies" in ISU rulebooks), wherein skaters perform jumps, spins, step sequences, spirals, and other elements in their programs.

男子单人和女子单人（国际滑联规则手册中称为&女士单人&）项目中，选手在节目中表演跳跃、旋转、步法顺序、连续旋转和其他各种姿态。

In the time domain, generation and propagation of aerodynamic noise are simulated using the two-step method and CAA respectively .

在时域内分别采用&两步法&及计算气动声学方法对气动噪声的产生及传播进行了数值模拟。

At Sudbury Valley School, students from preschool through high school age explore the world freely, at their own pace and in their own unique ways.

在Sudbury Valley School，从学前到中等教育的儿童都能根据他们个人的步法和独特的方式来自由的探索世界。

Liao source building glass products cover a number of types of building glass, the existing production lines, including two of the level of steel line, two lines of bending steel, a Low-E glass fiber reinforced plastic of line, two folders plastic glass production line, a hollow glass production line A GLAZE glass production lines, two bending glass production line, two one-step color folder folder wire glass production lines, three bilateral milling machine, automatic glass cutting machine Paul Quintero, many Units milling machine, positioning drilling machine, different line milling machine, Beveling machine, such as production equipment.

燎源建筑玻璃产品涵盖了多个种类的建筑用玻璃，现有生产线包括两条水平钢化线、两条弯钢化线、一条Low-E玻璃钢化线、两条夹胶玻璃生产线、一条中空玻璃生产线、一条彩釉玻生产线、两条热弯玻璃生产线、两台一步法彩夹夹丝玻璃生产线、三台双边磨边机、全自动保特罗玻璃切割机，多台磨边机、定位钻孔机、异行磨边机、斜边机等生产设备。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失