步法

In order to reduce the non-specific binding between oligonucleotides and bases mutation caused by the complicate secondary structure of DNA and excessive PCR amplification, a frequently phenomenon in one-step gene synthesis, we used a two-step method including assembly PCR and digestion-ligation step to synthesis Aspergillus niger lipase gene lipA.

本研究主要针对一步法长片段基因合成中复杂结构的非特异性配对和过多的PCR引入碱基错配等问题，采用二步法(组装PCR和酶切-酶连)合成了黑曲霉脂肪酶基因lipA。

In this thesis, PESA was synthesized using two different methods: the conventional one-step method and the two-step method we have developed. The corresponding products obtained by the one-step method and the two-step method are named PESA I and PESA II, respectively.

本文采用传统的一步法和非传统的两步法合成了PESA Ⅰ和PESA Ⅱ两种不同的产物，并通过单因素和正交试验确立了最佳合成路线。

The production technology of triethyl ortho - formate as medicine intermediate is reviewed.

综述了医药中间体原甲酸三乙酯的生产工艺，重点介绍了二步法、一步法、氢氰酸法、酯交换法和相转移催化法的合成原理与生产工艺，并对这几种生产工艺进行了评述。

Several methods applied widely for synthesizing uronic acid are introduced, such as methods of one-step process and two-step process.

分别就糖醛酸的几种常用合成方法进行了介绍，即直接将羟甲基氧化成羧基的一步法和先将羟甲基氧化成甲酰基、再氧化成羧基的两步法。

Record gait and procedures taught and client's ability to perform gaits in nurse's notes.

记录所教步法及程序及病人实施步法的能力。

The moderate packaging and environmental protection thoughts were inosculated into protection packaging design method,then the cushioning packaging design method based on moderate packaging evaluation system was developed.

探析&五步法&和&六步法&的异同点和不足之处，将适度包装和环境保护理念融入包装防护设计，提出基于适度包装评价体系的缓冲包装设计方法。

The length of the phase 1 maneuvers increase and the length of the phase 2 maneuvers decrease.

第1阶段的步法长度增加了，但第2阶段的步法减少了。

Three-dimensionally braided preforms can be categorized according to their manufacturing techniques.

三维编织织物按照它们的制造工艺，可分为二步法、四步法和实体编织。

The dynamic surface controller design method of the nonlinear autopilot for high maneuver missile is developed. Based on the smooth second-order dynamic sliding mode, the designed virtual controls are smooth enough and needn't smooth by integral filters when designing the inner loop controller with back-stepping method. So it overcomes the problem of explosion of terms.

提出了高机动非线性导弹自动驾驶仪的动态面控制器设计方法，该方法基于光滑二阶动态滑模，所设计的虚拟控制足够光滑，在利用反步法设计内环控制器时不需要进行积分滤波克服了反步法所带来的项数膨胀问题。

For different processing properties of plastics, the company developed a two-step, respectively, function bottle-blowing machine, two-step automatic Stretch Blow Molding Machine, Automatic Blow Molding Machines, large hollow blow molding machines, injection stretch blow molding machine and other series molding machinery, to meet different customer needs.

针对不同塑料的加工特性，公司分别开发了二步法多功能吹瓶机、二步法全自动吹瓶机、全自动中空成型机、大型中空吹塑机、注拉吹成型机等各系列成型机械，以满足不同客户的需求。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失