步法

Used the method of touch down PCR, the ACS2 gene was cloned from DNA of Petunia hybrida. At the same time, its gene sequence DQ090003 was obtained in GenBank, the antisense repetition of ACS2 linked the plasmid pBI-121 by use of one step. In the result, the expression vector having antisense repetition gene was constucted.

利用降落PCR的方法，从矮牵牛DNA中扩增出ACS2基因片段，在GenBank中获得基因序列号为DQ090003；利用一步法将ACS2基因的反义重复与pBI-121质粒连接，构建含该基因的反义重复表达载体。

Above can be called "three-step method."

以上可称为&三步法&。

Professional development and production "IBM" three-position series of one-step injection molding machines and blowing hollow molds, the company is involved in medicine, food, beverages, cosmetics packaging industry, exported to Southeast Asia, the Middle East, Europe and the United States, Africa and other countries and regions.

专业研制生产&I.B.M&系列一步法三工位注吹中空成型机及模具，产品涉及医药，食品，饮料，化妆品等包装行业，远销东南亚，中东，欧美，非洲等国家和地区。

Women players have already channged the normal way that sticking long in the same place in play. So they can exert gait at will now ,all that are benifited from the changes of the rules of the game as well as the reform of their dress.

女选手们早已不用固守在原地进行比赛，她们在场上可以随心所欲的展示自己的步法，这得益于比赛规则的变化，但同时与网球服装的改革也有密切的关系。

One step method of applying silane crosslinked PE technology to aluminium and plastics laminated pipes manufacturing process combines thin aluminium sheet high speed welding and polymer reaction extrusion together,setting up new pipe processing technology.

一步法硅烷交联聚乙烯管材加工技术应用于铝塑复合管成型加工，是将薄壁金属管高速焊接和聚合物反应挤出成型这两个跨专业的新技术有机地结合为一体，从而建立起一种加工新型管材的新兴工业技术。

Sodium arsenite group and sodium arsenate group were made by 300 mg/L per day of sodium arsenite and sodium arsenate respectively. The mice were sacrificed after 10 months for liver function and pathologic examination. The expression of MMp-8, TIMP-2, Col-Ⅰ、Col-Ⅲ mRNA were detected with real time flourescence quantitative PCR. The result was controlled with 18s.

亚砷酸钠组和砷酸钠组给予普通饲料和砷溶液。10个月后处死小鼠，血清进行肝功能检测；苏木素－伊红染色部分肝组织；Trizol-酚－氯仿一步法提取小鼠肝组织总RNA，紫外分光光度法测定总RNA浓度及纯度，实时荧光定量PCR法测肝组织中MMP-8、MMP-2、Col-Ⅰ、Col-Ⅲ mRNA的表达，以18S基因作为质控。

For the two-step bottle blowing machine supporting the entire embryo out machine, machine standard configuration includes: hopper, hoist bottle, bottle embryo out the whole machine, bottle slide rail embryo.

为两步法吹瓶机配套的瓶胚整列机，整机标准配置包括：料斗、瓶胚提升机、瓶胚整列机、瓶胚下滑导轨。

The intervention can use telephone consultation and face-to-face assistance, the operation method can use six-foot method.

失恋危机的干预可运用电话咨询、面询等方式，操作方法可用六步法。

By combining topological scan and definition of reasonable paths, the numbers of shortest path calculation is significantly reduced and improved versions of classical Dial's single-pass algorithm are presented. The efficiency of Dial's algorithm remains, while the definition of "reasonable paths" is improved.

算法将基于拓扑遍历的最短路算法与合理路径的选择相结合，有效减少了最短路的计算次数，提出了与经典Dial算法的单步法计算工作量相等的算法，改进了合理路径的定义并提高了计算精度。

The process in which iron ore pellets are pre-reduced in a shaft furnace and further reduced in an iron bath reactor is a very competitive smelting reduction process.

以球团矿为原料，采用竖炉预还原和铁浴终还原相结合的二步法流程是一种极具竞争力的熔融还原流程。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失