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Coli pET32/IL-11/BL(DE21 that expressed recombinant human interleukin-11 in the flask have been studied by orthogonal experiment design, including the components of culture medium (glucose, tryptone, yeast extract and pH), shaking speed, medium volume and inoculum concentration.

为了探讨发酵条件对大肠杆菌表达人白细胞介素-11融合蛋白的影响,利用正交实验设计,对影响工程菌的生长及重组人白细胞介素11融合蛋白的表达因素如培养基中的葡萄糖浓度、蛋白胨浓度、酵母提取物浓度、无机盐浓度、pH及摇床转速、装液量、接种量等在摇瓶中进行了初步的研究。

Based on the investigation on the various results of the race YUNCAN 7 and YUNCAN 8 and analysis on the result items, the found shown the effect of cocoon number per kg on the some items of cocoon production and egg production, that was negative relation between cocoon number per kg and cocoon production per g of new hatched larvae, egg production per g of new hatched larvae, and hatch-ing percentage; positive relation between cocoon number per kg and egg number per g and no effect on other items.

对云南省桑蚕普种场2005年春季不同生产小组饲育的云蚕7、云蚕8蚕品种各项成绩进行了综合调查,通过对其调查的有关项目数据进行分析总结,发现蚕茧成绩中的&kg茧粒数&对蚕茧成绩中和蚕种成绩中的部分项目产生了影响,即:&kg茧粒数&与&g蚁收茧量、g蚁制种量、孵化率&呈负相关性,&kg茧粒数&与&g卵粒数&则呈正相关性。而对其它一些项目则无影响。

Regularities among water color,transparency,plankton and DO were summarized on the base of the measured data of dozens of winter spending fish ponds in Heilongjiang and Jilin zones: 1 Transparency is negatively correlated with phytoplanktonic biomass,but positively with zooplanktonic biomass.

根据黑龙江、吉林地区数十个鱼类越冬池的实测数据,从水色、透明度、浮游生物与水体溶氧间的相互关系,总结如下规律:1 水体透明度与浮游植物量负相关,与浮游动物量正相关。

The life span of the adult and the ovipositing period may be quite long and there are two peaks of ovipositing activity separated by a period of about five days and each lasting about ten days.

渴稻虱为害水稻所造成的损失与每穴实际虫量成正相关,其迴归方程为y=0.39+0.31x(r=0.92),在同虫量相同虫龄的为害下,中梗受害最烈,单杂次之,单晚最轻。

The high xylanase producing strain parasitizing on the shelling fresh ramie was screened in the paper.The single factor experiment is carried out on the various carbon sources and density, the various nitrogen and density,the initial pH, the quantity of vaccination,the fermentation temperature and time.

摘 要:本文以去皮的新鲜苎麻为原料,筛选产木聚糖酶的高产寄生菌株,对其碳源种类及浓度,氮源种类及浓度,初适pH值、接种量、发酵温度及时间进行了单因素的试验,并选取影响显著的pH值、接种量、发酵温度及时间进行四因素三水平的正交实验。

The results showed:Under the same fertilizer consumption,covered with straw and covered with film can increase the chlorophyll content and total leer area duration of the maize,can improve leaf nitrate reeducates activity,Photosynthesis and roots vigor,and the nitrate reeducates activity,the chlorophyll content and the roots vigor present positive correlation with yield. Meanwhile,average dry matter accumulation of straw-covered increased 7.1% than this of no-ploughed,yield increased 15.3%.The average dry matter accumulation of film-covered increased 20.1% than which of no-ploughed and 12.1% than which of plough and covered with straws.And yield increased 26.8% and 10.1%,notable differences.

结果表明:在相同的肥料用量条件下,秸秆覆盖和薄膜覆膜对玉米生长发育具有良好的促进作用,能显著增加玉米叶片叶绿素含量和光合势,增强叶片硝酸还原酶活性和根系活力,同时玉米叶片硝酸还原酶活性、叶绿素含量、根系活力等与最终籽粒产量呈正相关;秸秆覆盖比免耕全生育期平均单株干物质积累量增加7.1%,籽粒产量增加15.3%,薄膜覆膜分别比免耕和翻耕残茬覆盖全生育期平均单株干物质积累量增加20.1%和12.1%,最终籽粒产量增加26.8%和10.1%,差异显著。

Moreover, the urea vaporizes the pH of quantity and soil to present a just related relation in the ammonia in the soil, when the ammonia vaporizes to have great capacity, the pH of the soil is worth to also go up.

另外,尿素在土壤中的氨挥发量与土壤的pH呈正相关的关系,氨挥发量大时,土壤的pH值也升高。

This paper discusses the addition amount of buckwheat powder, baking powder and the effect on buckwheat microwave cake which the time of microwave heating has through orthogonal experiment, and determines the optimal formula and processing parameters of microwave buckwheat cake.

本研究通过正交实验探讨苦荞粉添加量、泡打粉添加量、微波加热时间等对苦荞微波蛋糕品质的影响,确定了苦荞微波蛋糕的最佳配方和加工工艺参数。

After rescreened,a strain F00 (named temporarily F00, the same to the following),with highest flocculating activity was picked up.Using optimized and orthogonal tests, the optimum culture conditions are: Sugar as source of carbon, Cargamide as source of nitrogen,added P, Mg, K, Zn, Mn mineral elements, initial pH value 6.5, inoculation 4-5 x 104spores/ ml, temperature 30癈 and aerations 80 rpm.

通过用它作接种量、培养基优化及最佳发酵条件正交试验,确定F_(00)产生絮凝剂最佳条件:发酵培养基以蔗糖作碳源,尿素作氮源,辅加磷、镁、钾、锌、锰等矿质元素,初始pH值6.5,接种量为每毫升发酵液起始含4~5×10~4个孢子,发酵温度30℃,摇床转速80rpm。

Extract of total flavonoids was purified with macroeticular resins. Different kinds of resins were applied to inspect the static and dynamic adsorption ability,to optimize the concentration,volume and flow rate of eluant.

对葛根总黄酮的提取纯化工艺进行了研究,考察不同乙醇浓度,溶媒倍量,提取时间、提取次数和提取温度对总黄酮转移率和纯度的影响,建立了四因素三水平的正交试验,最终确立了最佳的提取工艺为:10倍量70%乙醇加热回流提取2次,每次2小时。

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