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The contents of myelin basic protein in cerebrospinal; fluid , brain tissues and plasma and the MBP-mRNA expression levels in hypothalamus, hippocampus and temporoparietal grey and white matters were determined with ELISA and hybridization respectively.

结果显示:致伤后8h,低、高速组脑组织、血清、CSF中MBP含量均显著高于对照组和致伤前(P<0.05,P<0.01),脑组织中以丘脑下部和海马区MBP含量升高最显著(P<0.01),血清、CSF中MBP含量与脑组织中MBP含量呈显著正相关(P<0.05,P<0.01);低速组丘脑下部MBP-mRNA表达显著高于对照组(P<0.05),高速组海马、丘脑下部MBP-mRNA表达均极显著高于对照组和低速组(P<0.01)。

In order to study the mechanism of the inhibitory effect of salvia miltiorrhiza and tetramethyl pyrazine on scar fibroblast, the DNA content of fibroblast and the all distribution in cellular cycle was measured by FCM. The hypertrophic scar tissue of chest was chosen for primary culture of fibroblast.

摘 要 应用流式细胞光度分析法测定丹参和川芎嗪对体外培养的瘢痕成纤维细胞DNA相对含量和细胞周期时相变化,发现药物在一定浓度作用下,DNA指数无明显变化;丹参使C2-M期细胞分布增多,G2-M期延长;川芎嗪使G2-M期细胞分布增多,G2-M期、S期延长;药物使细胞群体倍增时间延长,与浓度呈明显直线正相关

For the first time a safety and nontoxic solvent were used to gain the crystal of teasaponins,avoiding environmental contaminated of toxical impregnate and receiving the high purity teasaponin products.

茶皂素粗提物对食品腐败细菌、真菌以及植物病原真菌的抗菌实验结果表明,茶皂素对各种细菌和真菌均有不同程度的抑制作用,且抑菌效果与茶皂素浓度呈正相关

Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2~3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.

本文将对氧化还原状态变化敏感的绿色荧光蛋白roGFP1-R12,在酵母细胞中实现了多拷贝强表达;荧光扫描经强氧化剂H_2O_2和还原剂DTT以及环境中重金属NaAsO_2或Pb(NO_3)_2处理后的酵母细胞悬液,测定510 nm处的荧光发射强度结果显示,表达的绿色荧光蛋白对氧化还原水平敏感,且在510 nm处的荧光强度与一定的重金属浓度呈正相关,即roGFP1-R12在510nm处的荧光发射值随重金属浓度的增高而增强,从而说明重金属对细胞的毒性在一定程度上很可能是通过破坏细胞内的氧化还原平衡发生作用;同时通过该绿色荧光蛋白对胞内氧化还原状态变化的响应情况可以来实时检测环境中的重金属;遗传学的点突变频率及致死率实验数据表明,重金属能导致菌体的点突变频率和致死率升高,且活性氧的清除剂巯基脲能明显降低这种点突变和致死率,说明由重金属引发的这种点突变和致死效应在很大程度上是依赖于重金属对细胞诱导产生的氧化胁迫。

Histochemically, the results indicated that POD activity increased with the growth rate of callus within the same tissue; and lignin deposit density within the cell wall increased with the growth of callus over time, displaying a trend similar to POD activity alteration upon Hg(superscript 2+) imposition; Furthermore, more tracheary elements was observed in the callus tissue subjected to Hg(superscript 2+) than that in control.

组织化学定位结果表明:同一处理样品的过氧化物酶活性随着愈伤组织的生长进程而逐渐增强,且与Hg(上标 2+)浓度呈正相关;细胞壁中木质素沉积随着愈伤组织的生长而增加,与过氧化物酶活性变化趋势一致;Hg(上标 2+)处理的愈伤组织中导管诱导率大于对照。

Conclusions1、E_2 may induce the apoptosis and Fas,FasL expressions of Jurkat cell,its effect positively related to the dose and treating time of E_2.2、There is intrinsic co-expression of ERαand ERβon Jurkat cell,which can be upregulated by E_2,positively related to E_2 doses.3、E_2 may act as an apoptosis inducer of Jurkat cell via ERβ.4、E_2 can promote the transcriptive activity of NF-κB in Jurkat cell.The increased NF-κB activity may lead to increased apoptosis of Jurkat cell.

结论1、E_2可增加Jurkat细胞的凋亡,并增加Fas、FasL的蛋白表达,且其作用与E_2的剂量和处理时间呈正相关。2、Jurkat细胞上存在ERα、ERβ的表达,且E_2可上调这两种受体的表达,E_2剂量越大,ER表达量越高。3、E_2可能通过ERβ发挥其促进Jurkat细胞凋亡的作用。4、E_2能增加Jurkat细胞中NF-κB的转录活性,Jurkat细胞中NF-κB活性增加有促进凋亡的作用。

The relationships of VEGF with the indices of renal damage, including renal/body weight, urinary protein excretion, glomerular volume and glomerular area, were analyzed. Results The expression of VEGF mRNA in diabetic kidney was significantly up-regulated after operation from 2 weeks to 24 weeks with the peak level at 20weeks, when compared with control at the same time- points. The positive results of VEGF staining in diabetic glomeruli was increasingly observed after operation from 2 weeks to 24 weeks, with the peak at 20 weeks. The positive results of VEGF staining in diabetic tubuli was increasingly seen from 2 weeks to 24 weeks, with the peak at 8 weeks.

结果 VEGF mRNA在糖尿病模型组大鼠肾脏中持续高表达,各时点与对照组相比差异均有统计学意义(P.05);糖尿病组2~24周肾小球VEGF蛋白表达强于对照组(P.05),24周时有所减弱,但仍强于对照组;糖尿病组各时点肾小管VEGF蛋白表达均强于对照组(p.05), 8周后表达明显增强;VEGF表达变化与肾质量/体质量(r=0.518, P=0.001)、尿蛋白(r=0.409, p=0.001)、肾小球面积(r=0.499, P-0.000)和体积(r=0.499, P-0.000)呈正相关

4The relationship between senior management share and debt financing is unapparent.

高级管理人员持股比例与债务融资比例没有显著的正相关关系。

The vasomotion frequency and amplitude was positivelycorrelated with CPB flow rate.

血管运动频率和幅度与 CPB 流速呈正相关

The success rate in showing the mid-sagittal plan of the cerebellar vermis (the plan with the largest vermian area) in different way was compared.

应用三维超声第三平面成像测量小脑蚓部前后径、上下径和最大切面面积均与孕周呈正相关

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?