正常体

Tillet and Francis (1930) discovered an unidentified substance in the sera of patients with pneumonia which was precipitated with the C-fraction polysaccharide of pneumococci.

本研究采取596个正常成熟新生儿（怀孕周数为38至40周，出生体重大於2，500公克，Apgar分数皆为8分以上）之血清为对象以定量其血中CRP之值。

Methods. Lateral full-length radiographs of 18 normal volunteers were examined.

方法。我们对18个正常志愿者进行了椎体的外侧全长影像学检查。

Normally the lysosomes may be protecting the cell from the deleterious effects of such ions, by sequestering them.

在正常情况下，容酶体将这些金属离子隔离，保护细胞免受其有害的作用。

Normally the lysosomes may be protecting the cell from the deleterious effects of such metal ions, by sequestering them

在正常情况下，溶酶体将这些金属离子隔离，保护细胞免受其有害的作用。

In this example , 2001 and Mountain Bikes are in bold text since they represent subtotals whereas the individual mountain bike models are in regular text since they are at the lowest level of detail .

在这个例子中，因为2001和山地自行车后面显示小计的结果，所以这里以粗体字体显示，而山地自行车的款式则以正常的字体显示，因为它们在明细数据中等级最低。

Methods Using modified Ferriers ischemia-reperfusion induced arrhythmic model in isolated guinea pig right ventricular wall.In this model,the preparation was superfused with modified ischemic Tyrodes solution for 10 min then reperfusioned with normal Tyrodes solution.

修改的Ferrier豚鼠离体右心室壁缺血-再灌注心律失常模型，即标本经模拟缺血台氏液灌流10min，而后用正常台氏液行再灌注。

Three days after local cerebral infarction , the amounts of catecholamine-containing varicosities and the concentrations of CA in the varicosities in cortices of both sides were markedly reduced, and an aggregation of a large amount of CA granules which were of different fluorescent feature from tha...

局壮性脑梗塞三天后，双侧大脑皮质内CA膨体的数量与膨体内CA的浓度均显著减少，梗塞灶边缘区可见大量CA颗粒聚集，其荧光特征与正常皮质内的CA颗粒不同；双侧大脑皮质内的去甲肾上腺素含量显著减少，多巴胺含量无明显变化。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Varieties: There is a colour variety, brick red instead of the normal brown-red.

变异：有一个颜色变体，红棕色取代了正常的红褐色。

L-arginine, an NO precursor, reduced the contractile amplitude and end-diastolic cell length in the cardiomyocyte; but reversibly increased LVDP,±dp/dtmax, and coronary flow in isolated perfused rat heart.

2NO的前体L-精氨酸(L-arginine， L-Arg)引起心肌细胞舒张期细胞长度缩短、收缩幅度降低。离体灌流心脏LVDP、冠脉流量、和±dp/dtmax增高，用K-H液复灌后可恢复正常。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。